Experiment: R2A with Lomefloxacin 0.00075 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30165 and RR42_RS30170 are separated by 123 nucleotides RR42_RS30170 and RR42_RS30175 are separated by 154 nucleotides
RR42_RS30165: RR42_RS30165 - membrane protein, at 2,141,524 to 2,142,720
_RS30165
RR42_RS30170: RR42_RS30170 - aldolase, at 2,142,844 to 2,143,608
_RS30170
RR42_RS30175: RR42_RS30175 - beta-glucosidase, at 2,143,763 to 2,145,889
_RS30175
Position (kb)
2142
2143
2144 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2141.924 kb on + strand, within RR42_RS30165 at 2141.924 kb on + strand, within RR42_RS30165 at 2141.925 kb on - strand, within RR42_RS30165 at 2141.925 kb on - strand, within RR42_RS30165 at 2142.029 kb on + strand, within RR42_RS30165 at 2142.029 kb on + strand, within RR42_RS30165 at 2142.030 kb on - strand, within RR42_RS30165 at 2142.054 kb on + strand, within RR42_RS30165 at 2142.055 kb on - strand, within RR42_RS30165 at 2142.143 kb on + strand, within RR42_RS30165 at 2142.144 kb on - strand, within RR42_RS30165 at 2142.182 kb on + strand, within RR42_RS30165 at 2142.294 kb on - strand, within RR42_RS30165 at 2142.294 kb on - strand, within RR42_RS30165 at 2142.294 kb on - strand, within RR42_RS30165 at 2142.294 kb on - strand, within RR42_RS30165 at 2142.512 kb on - strand, within RR42_RS30165 at 2142.626 kb on + strand at 2142.626 kb on + strand at 2142.626 kb on + strand at 2142.627 kb on - strand at 2142.627 kb on - strand at 2142.627 kb on - strand at 2142.627 kb on - strand at 2142.705 kb on - strand at 2142.705 kb on - strand at 2142.705 kb on - strand at 2142.709 kb on - strand at 2142.709 kb on - strand at 2142.709 kb on - strand at 2142.730 kb on + strand at 2142.730 kb on + strand at 2142.733 kb on + strand at 2142.734 kb on - strand at 2142.734 kb on - strand at 2142.845 kb on + strand at 2142.846 kb on - strand at 2142.846 kb on - strand at 2143.034 kb on + strand, within RR42_RS30170 at 2143.034 kb on + strand, within RR42_RS30170 at 2143.035 kb on - strand, within RR42_RS30170 at 2143.035 kb on - strand, within RR42_RS30170 at 2143.035 kb on - strand, within RR42_RS30170 at 2143.266 kb on + strand, within RR42_RS30170 at 2143.272 kb on + strand, within RR42_RS30170 at 2143.272 kb on + strand, within RR42_RS30170 at 2143.600 kb on - strand at 2143.630 kb on + strand at 2143.731 kb on + strand at 2143.793 kb on + strand at 2143.793 kb on + strand at 2143.793 kb on + strand at 2143.794 kb on - strand at 2143.915 kb on + strand at 2144.045 kb on - strand, within RR42_RS30175 at 2144.118 kb on + strand, within RR42_RS30175 at 2144.118 kb on + strand, within RR42_RS30175 at 2144.118 kb on + strand, within RR42_RS30175 at 2144.118 kb on + strand, within RR42_RS30175 at 2144.119 kb on - strand, within RR42_RS30175 at 2144.202 kb on + strand, within RR42_RS30175 at 2144.323 kb on + strand, within RR42_RS30175 at 2144.539 kb on - strand, within RR42_RS30175
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM remove 2,141,924 + RR42_RS30165 0.33 +0.1 2,141,924 + RR42_RS30165 0.33 +0.0 2,141,925 - RR42_RS30165 0.34 -0.4 2,141,925 - RR42_RS30165 0.34 -0.1 2,142,029 + RR42_RS30165 0.42 +1.1 2,142,029 + RR42_RS30165 0.42 +0.6 2,142,030 - RR42_RS30165 0.42 +0.3 2,142,054 + RR42_RS30165 0.44 +0.9 2,142,055 - RR42_RS30165 0.44 -0.0 2,142,143 + RR42_RS30165 0.52 -0.3 2,142,144 - RR42_RS30165 0.52 -1.1 2,142,182 + RR42_RS30165 0.55 -0.0 2,142,294 - RR42_RS30165 0.64 +0.2 2,142,294 - RR42_RS30165 0.64 +0.9 2,142,294 - RR42_RS30165 0.64 -0.5 2,142,294 - RR42_RS30165 0.64 +0.6 2,142,512 - RR42_RS30165 0.83 +0.2 2,142,626 + -0.5 2,142,626 + -2.2 2,142,626 + -1.1 2,142,627 - -0.9 2,142,627 - +0.5 2,142,627 - -0.1 2,142,627 - +0.0 2,142,705 - +0.0 2,142,705 - -0.8 2,142,705 - +0.5 2,142,709 - -0.4 2,142,709 - -0.2 2,142,709 - -0.7 2,142,730 + +0.2 2,142,730 + +2.5 2,142,733 + +1.7 2,142,734 - +0.6 2,142,734 - -0.4 2,142,845 + -1.1 2,142,846 - +0.3 2,142,846 - -1.3 2,143,034 + RR42_RS30170 0.25 -0.2 2,143,034 + RR42_RS30170 0.25 +0.6 2,143,035 - RR42_RS30170 0.25 +0.3 2,143,035 - RR42_RS30170 0.25 +0.9 2,143,035 - RR42_RS30170 0.25 -0.6 2,143,266 + RR42_RS30170 0.55 -0.1 2,143,272 + RR42_RS30170 0.56 +1.9 2,143,272 + RR42_RS30170 0.56 +1.3 2,143,600 - -0.7 2,143,630 + -0.1 2,143,731 + -0.5 2,143,793 + +0.1 2,143,793 + -0.4 2,143,793 + +1.2 2,143,794 - +0.6 2,143,915 + -1.4 2,144,045 - RR42_RS30175 0.13 -0.4 2,144,118 + RR42_RS30175 0.17 -0.9 2,144,118 + RR42_RS30175 0.17 -0.1 2,144,118 + RR42_RS30175 0.17 +0.5 2,144,118 + RR42_RS30175 0.17 +0.6 2,144,119 - RR42_RS30175 0.17 +1.2 2,144,202 + RR42_RS30175 0.21 +0.9 2,144,323 + RR42_RS30175 0.26 -0.4 2,144,539 - RR42_RS30175 0.36 -0.3
Or see this region's nucleotide sequence