Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS29260

Experiment: R2A with Lomefloxacin 0.00075 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS29250 and RR42_RS29255 are separated by 174 nucleotidesRR42_RS29255 and RR42_RS29260 are separated by 129 nucleotidesRR42_RS29260 and RR42_RS29265 are separated by 7 nucleotides RR42_RS29250: RR42_RS29250 - nitrile hydratase, at 1,916,336 to 1,916,722 _RS29250 RR42_RS29255: RR42_RS29255 - GTP cyclohydrolase, at 1,916,897 to 1,917,493 _RS29255 RR42_RS29260: RR42_RS29260 - hypothetical protein, at 1,917,623 to 1,918,972 _RS29260 RR42_RS29265: RR42_RS29265 - hypothetical protein, at 1,918,980 to 1,920,128 _RS29265 Position (kb) 1917 1918 1919Strain fitness (log2 ratio) -2 -1 0 1 2at 1916.744 kb on + strandat 1916.825 kb on - strandat 1916.837 kb on + strandat 1916.845 kb on - strandat 1916.960 kb on - strand, within RR42_RS29255at 1916.960 kb on - strand, within RR42_RS29255at 1917.164 kb on + strand, within RR42_RS29255at 1917.164 kb on + strand, within RR42_RS29255at 1917.218 kb on + strand, within RR42_RS29255at 1917.219 kb on - strand, within RR42_RS29255at 1917.219 kb on - strand, within RR42_RS29255at 1917.245 kb on - strand, within RR42_RS29255at 1917.293 kb on + strand, within RR42_RS29255at 1917.294 kb on - strand, within RR42_RS29255at 1917.294 kb on - strand, within RR42_RS29255at 1917.418 kb on + strand, within RR42_RS29255at 1917.418 kb on + strand, within RR42_RS29255at 1917.419 kb on - strand, within RR42_RS29255at 1917.757 kb on + strandat 1917.777 kb on - strand, within RR42_RS29260at 1917.810 kb on - strand, within RR42_RS29260at 1917.924 kb on - strand, within RR42_RS29260at 1917.947 kb on + strand, within RR42_RS29260at 1917.947 kb on + strand, within RR42_RS29260at 1917.947 kb on + strand, within RR42_RS29260at 1917.947 kb on + strand, within RR42_RS29260at 1917.948 kb on - strand, within RR42_RS29260at 1917.948 kb on - strand, within RR42_RS29260at 1917.948 kb on - strand, within RR42_RS29260at 1917.953 kb on + strand, within RR42_RS29260at 1917.953 kb on + strand, within RR42_RS29260at 1917.953 kb on + strand, within RR42_RS29260at 1917.954 kb on - strand, within RR42_RS29260at 1917.954 kb on - strand, within RR42_RS29260at 1917.954 kb on - strand, within RR42_RS29260at 1917.954 kb on - strand, within RR42_RS29260at 1917.954 kb on - strand, within RR42_RS29260at 1917.954 kb on - strand, within RR42_RS29260at 1917.954 kb on - strand, within RR42_RS29260at 1917.954 kb on - strand, within RR42_RS29260at 1917.954 kb on - strand, within RR42_RS29260at 1918.085 kb on + strand, within RR42_RS29260at 1918.086 kb on - strand, within RR42_RS29260at 1918.091 kb on + strand, within RR42_RS29260at 1918.091 kb on + strand, within RR42_RS29260at 1918.091 kb on + strand, within RR42_RS29260at 1918.091 kb on + strand, within RR42_RS29260at 1918.092 kb on - strand, within RR42_RS29260at 1918.092 kb on - strand, within RR42_RS29260at 1918.335 kb on - strand, within RR42_RS29260at 1918.805 kb on + strand, within RR42_RS29260at 1918.805 kb on + strand, within RR42_RS29260at 1918.904 kb on + strandat 1918.904 kb on + strandat 1918.904 kb on + strandat 1919.364 kb on + strand, within RR42_RS29265at 1919.491 kb on + strand, within RR42_RS29265at 1919.492 kb on - strand, within RR42_RS29265at 1919.492 kb on - strand, within RR42_RS29265at 1919.653 kb on - strand, within RR42_RS29265at 1919.653 kb on - strand, within RR42_RS29265at 1919.663 kb on + strand, within RR42_RS29265at 1919.670 kb on - strand, within RR42_RS29265at 1919.725 kb on + strand, within RR42_RS29265at 1919.725 kb on + strand, within RR42_RS29265at 1919.726 kb on - strand, within RR42_RS29265at 1919.844 kb on + strand, within RR42_RS29265at 1919.852 kb on + strand, within RR42_RS29265at 1919.852 kb on + strand, within RR42_RS29265at 1919.852 kb on + strand, within RR42_RS29265at 1919.872 kb on + strand, within RR42_RS29265

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM
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1,916,744 + -0.5
1,916,825 - -0.8
1,916,837 + +0.2
1,916,845 - +0.9
1,916,960 - RR42_RS29255 0.11 -0.6
1,916,960 - RR42_RS29255 0.11 -0.0
1,917,164 + RR42_RS29255 0.45 -1.1
1,917,164 + RR42_RS29255 0.45 +0.2
1,917,218 + RR42_RS29255 0.54 +0.5
1,917,219 - RR42_RS29255 0.54 +0.3
1,917,219 - RR42_RS29255 0.54 -0.5
1,917,245 - RR42_RS29255 0.58 -1.1
1,917,293 + RR42_RS29255 0.66 -0.8
1,917,294 - RR42_RS29255 0.66 -0.7
1,917,294 - RR42_RS29255 0.66 +0.2
1,917,418 + RR42_RS29255 0.87 -2.1
1,917,418 + RR42_RS29255 0.87 +0.9
1,917,419 - RR42_RS29255 0.87 -1.5
1,917,757 + +0.3
1,917,777 - RR42_RS29260 0.11 -2.3
1,917,810 - RR42_RS29260 0.14 +0.3
1,917,924 - RR42_RS29260 0.22 +0.4
1,917,947 + RR42_RS29260 0.24 -1.7
1,917,947 + RR42_RS29260 0.24 +1.5
1,917,947 + RR42_RS29260 0.24 +0.2
1,917,947 + RR42_RS29260 0.24 +0.4
1,917,948 - RR42_RS29260 0.24 -0.5
1,917,948 - RR42_RS29260 0.24 +1.5
1,917,948 - RR42_RS29260 0.24 +0.3
1,917,953 + RR42_RS29260 0.24 -0.1
1,917,953 + RR42_RS29260 0.24 +0.1
1,917,953 + RR42_RS29260 0.24 +0.9
1,917,954 - RR42_RS29260 0.25 -0.1
1,917,954 - RR42_RS29260 0.25 +0.6
1,917,954 - RR42_RS29260 0.25 +1.6
1,917,954 - RR42_RS29260 0.25 -0.7
1,917,954 - RR42_RS29260 0.25 -0.2
1,917,954 - RR42_RS29260 0.25 -0.0
1,917,954 - RR42_RS29260 0.25 +1.9
1,917,954 - RR42_RS29260 0.25 +0.4
1,917,954 - RR42_RS29260 0.25 +0.8
1,918,085 + RR42_RS29260 0.34 +2.0
1,918,086 - RR42_RS29260 0.34 +0.9
1,918,091 + RR42_RS29260 0.35 +0.9
1,918,091 + RR42_RS29260 0.35 -1.1
1,918,091 + RR42_RS29260 0.35 +0.6
1,918,091 + RR42_RS29260 0.35 +0.3
1,918,092 - RR42_RS29260 0.35 +0.9
1,918,092 - RR42_RS29260 0.35 +1.7
1,918,335 - RR42_RS29260 0.53 +0.9
1,918,805 + RR42_RS29260 0.88 +0.5
1,918,805 + RR42_RS29260 0.88 -0.0
1,918,904 + +0.4
1,918,904 + +0.4
1,918,904 + +0.9
1,919,364 + RR42_RS29265 0.33 -2.5
1,919,491 + RR42_RS29265 0.44 -0.1
1,919,492 - RR42_RS29265 0.45 -1.6
1,919,492 - RR42_RS29265 0.45 -0.9
1,919,653 - RR42_RS29265 0.59 -0.7
1,919,653 - RR42_RS29265 0.59 +0.2
1,919,663 + RR42_RS29265 0.59 -2.6
1,919,670 - RR42_RS29265 0.60 -0.3
1,919,725 + RR42_RS29265 0.65 -0.3
1,919,725 + RR42_RS29265 0.65 -0.5
1,919,726 - RR42_RS29265 0.65 +0.9
1,919,844 + RR42_RS29265 0.75 +0.3
1,919,852 + RR42_RS29265 0.76 -2.1
1,919,852 + RR42_RS29265 0.76 -0.5
1,919,852 + RR42_RS29265 0.76 -0.1
1,919,872 + RR42_RS29265 0.78 +1.3

Or see this region's nucleotide sequence