Experiment: R2A with Lomefloxacin 0.00075 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28725 and RR42_RS28730 overlap by 4 nucleotides RR42_RS28730 and RR42_RS28735 overlap by 4 nucleotides RR42_RS28735 and RR42_RS28740 overlap by 8 nucleotides
RR42_RS28725: RR42_RS28725 - ABC transporter permease, at 1,795,839 to 1,796,705
_RS28725
RR42_RS28730: RR42_RS28730 - ABC transporter permease, at 1,796,702 to 1,797,718
_RS28730
RR42_RS28735: RR42_RS28735 - hemolysin III, at 1,797,715 to 1,798,467
_RS28735
RR42_RS28740: RR42_RS28740 - ABC transporter ATP-binding protein, at 1,798,460 to 1,799,161
_RS28740
Position (kb)
1796
1797
1798 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1795.881 kb on + strand at 1795.882 kb on - strand at 1795.882 kb on - strand at 1795.882 kb on - strand at 1795.909 kb on + strand at 1796.041 kb on + strand, within RR42_RS28725 at 1796.175 kb on + strand, within RR42_RS28725 at 1796.175 kb on + strand, within RR42_RS28725 at 1796.175 kb on + strand, within RR42_RS28725 at 1796.176 kb on - strand, within RR42_RS28725 at 1796.176 kb on - strand, within RR42_RS28725 at 1796.176 kb on - strand, within RR42_RS28725 at 1796.408 kb on - strand, within RR42_RS28725 at 1796.505 kb on + strand, within RR42_RS28725 at 1796.505 kb on + strand, within RR42_RS28725 at 1796.505 kb on + strand, within RR42_RS28725 at 1796.505 kb on + strand, within RR42_RS28725 at 1796.506 kb on - strand, within RR42_RS28725 at 1796.506 kb on - strand, within RR42_RS28725 at 1796.506 kb on - strand, within RR42_RS28725 at 1796.506 kb on - strand, within RR42_RS28725 at 1796.506 kb on - strand, within RR42_RS28725 at 1796.514 kb on + strand, within RR42_RS28725 at 1796.514 kb on + strand, within RR42_RS28725 at 1796.514 kb on + strand, within RR42_RS28725 at 1796.515 kb on - strand, within RR42_RS28725 at 1796.515 kb on - strand, within RR42_RS28725 at 1796.515 kb on - strand, within RR42_RS28725 at 1796.610 kb on + strand, within RR42_RS28725 at 1796.610 kb on + strand, within RR42_RS28725 at 1796.610 kb on + strand, within RR42_RS28725 at 1796.610 kb on + strand, within RR42_RS28725 at 1796.611 kb on - strand, within RR42_RS28725 at 1796.611 kb on - strand, within RR42_RS28725 at 1796.611 kb on - strand, within RR42_RS28725 at 1796.613 kb on + strand, within RR42_RS28725 at 1796.613 kb on + strand, within RR42_RS28725 at 1796.613 kb on + strand, within RR42_RS28725 at 1796.614 kb on - strand, within RR42_RS28725 at 1796.643 kb on + strand at 1796.643 kb on + strand at 1796.643 kb on + strand at 1796.643 kb on + strand at 1796.644 kb on - strand at 1796.644 kb on - strand at 1796.644 kb on - strand at 1796.644 kb on - strand at 1796.644 kb on - strand at 1796.951 kb on + strand, within RR42_RS28730 at 1796.951 kb on + strand, within RR42_RS28730 at 1796.951 kb on + strand, within RR42_RS28730 at 1796.952 kb on - strand, within RR42_RS28730 at 1796.952 kb on - strand, within RR42_RS28730 at 1796.952 kb on - strand, within RR42_RS28730 at 1797.230 kb on + strand, within RR42_RS28730 at 1797.524 kb on + strand, within RR42_RS28730 at 1797.524 kb on + strand, within RR42_RS28730 at 1797.524 kb on + strand, within RR42_RS28730 at 1797.524 kb on + strand, within RR42_RS28730 at 1797.525 kb on - strand, within RR42_RS28730 at 1797.593 kb on + strand, within RR42_RS28730 at 1797.594 kb on - strand, within RR42_RS28730 at 1797.732 kb on + strand at 1797.733 kb on - strand at 1797.934 kb on + strand, within RR42_RS28735 at 1797.935 kb on - strand, within RR42_RS28735 at 1797.967 kb on + strand, within RR42_RS28735 at 1797.968 kb on - strand, within RR42_RS28735 at 1797.968 kb on - strand, within RR42_RS28735 at 1798.341 kb on - strand, within RR42_RS28735 at 1798.341 kb on - strand, within RR42_RS28735 at 1798.439 kb on + strand at 1798.439 kb on + strand at 1798.441 kb on + strand at 1798.442 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM remove 1,795,881 + +0.8 1,795,882 - +0.0 1,795,882 - +1.9 1,795,882 - -0.8 1,795,909 + +1.0 1,796,041 + RR42_RS28725 0.23 -1.9 1,796,175 + RR42_RS28725 0.39 -0.6 1,796,175 + RR42_RS28725 0.39 -0.4 1,796,175 + RR42_RS28725 0.39 -0.1 1,796,176 - RR42_RS28725 0.39 -1.4 1,796,176 - RR42_RS28725 0.39 +0.4 1,796,176 - RR42_RS28725 0.39 +0.3 1,796,408 - RR42_RS28725 0.66 +0.3 1,796,505 + RR42_RS28725 0.77 -0.7 1,796,505 + RR42_RS28725 0.77 +0.8 1,796,505 + RR42_RS28725 0.77 -0.8 1,796,505 + RR42_RS28725 0.77 -1.1 1,796,506 - RR42_RS28725 0.77 +0.9 1,796,506 - RR42_RS28725 0.77 -0.6 1,796,506 - RR42_RS28725 0.77 -1.1 1,796,506 - RR42_RS28725 0.77 +0.3 1,796,506 - RR42_RS28725 0.77 -0.1 1,796,514 + RR42_RS28725 0.78 -1.3 1,796,514 + RR42_RS28725 0.78 -0.0 1,796,514 + RR42_RS28725 0.78 -0.7 1,796,515 - RR42_RS28725 0.78 -0.2 1,796,515 - RR42_RS28725 0.78 -0.7 1,796,515 - RR42_RS28725 0.78 +0.5 1,796,610 + RR42_RS28725 0.89 +0.3 1,796,610 + RR42_RS28725 0.89 -0.3 1,796,610 + RR42_RS28725 0.89 -0.9 1,796,610 + RR42_RS28725 0.89 +0.8 1,796,611 - RR42_RS28725 0.89 +0.4 1,796,611 - RR42_RS28725 0.89 -0.3 1,796,611 - RR42_RS28725 0.89 -0.6 1,796,613 + RR42_RS28725 0.89 -0.3 1,796,613 + RR42_RS28725 0.89 +0.9 1,796,613 + RR42_RS28725 0.89 -0.4 1,796,614 - RR42_RS28725 0.89 +0.4 1,796,643 + -1.1 1,796,643 + +0.5 1,796,643 + -0.6 1,796,643 + -0.9 1,796,644 - -0.0 1,796,644 - -1.9 1,796,644 - -1.2 1,796,644 - +0.4 1,796,644 - -0.4 1,796,951 + RR42_RS28730 0.24 -0.6 1,796,951 + RR42_RS28730 0.24 +0.7 1,796,951 + RR42_RS28730 0.24 -1.1 1,796,952 - RR42_RS28730 0.25 -0.7 1,796,952 - RR42_RS28730 0.25 +0.3 1,796,952 - RR42_RS28730 0.25 -0.8 1,797,230 + RR42_RS28730 0.52 +0.7 1,797,524 + RR42_RS28730 0.81 -1.1 1,797,524 + RR42_RS28730 0.81 -0.0 1,797,524 + RR42_RS28730 0.81 -1.1 1,797,524 + RR42_RS28730 0.81 -0.7 1,797,525 - RR42_RS28730 0.81 -0.7 1,797,593 + RR42_RS28730 0.88 -0.6 1,797,594 - RR42_RS28730 0.88 -0.6 1,797,732 + +1.4 1,797,733 - -0.2 1,797,934 + RR42_RS28735 0.29 -1.9 1,797,935 - RR42_RS28735 0.29 -2.6 1,797,967 + RR42_RS28735 0.33 -0.8 1,797,968 - RR42_RS28735 0.34 -0.2 1,797,968 - RR42_RS28735 0.34 -1.1 1,798,341 - RR42_RS28735 0.83 -1.2 1,798,341 - RR42_RS28735 0.83 -0.5 1,798,439 + +0.9 1,798,439 + -0.9 1,798,441 + +0.1 1,798,442 - +0.0
Or see this region's nucleotide sequence