Experiment: R2A with Lomefloxacin 0.00075 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28210 and RR42_RS28215 are separated by 148 nucleotides RR42_RS28215 and RR42_RS28220 are separated by 75 nucleotides RR42_RS28220 and RR42_RS28225 are separated by 146 nucleotides
RR42_RS28210: RR42_RS28210 - LysR family transcriptional regulator, at 1,685,773 to 1,686,699
_RS28210
RR42_RS28215: RR42_RS28215 - MBL fold metallo-hydrolase, at 1,686,848 to 1,687,753
_RS28215
RR42_RS28220: RR42_RS28220 - protein-disulfide isomerase, at 1,687,829 to 1,688,569
_RS28220
RR42_RS28225: RR42_RS28225 - diguanylate cyclase, at 1,688,716 to 1,690,893
_RS28225
Position (kb) 1686
1687
1688 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1685.891 kb on + strand, within RR42_RS28210 at 1685.891 kb on + strand, within RR42_RS28210 at 1685.892 kb on - strand, within RR42_RS28210 at 1685.892 kb on - strand, within RR42_RS28210 at 1685.892 kb on - strand, within RR42_RS28210 at 1685.892 kb on - strand, within RR42_RS28210 at 1686.119 kb on + strand, within RR42_RS28210 at 1686.126 kb on - strand, within RR42_RS28210 at 1686.126 kb on - strand, within RR42_RS28210 at 1686.129 kb on + strand, within RR42_RS28210 at 1686.130 kb on - strand, within RR42_RS28210 at 1686.130 kb on - strand, within RR42_RS28210 at 1686.130 kb on - strand, within RR42_RS28210 at 1686.188 kb on + strand, within RR42_RS28210 at 1686.188 kb on + strand, within RR42_RS28210 at 1686.188 kb on + strand, within RR42_RS28210 at 1686.189 kb on - strand, within RR42_RS28210 at 1686.189 kb on - strand, within RR42_RS28210 at 1686.189 kb on - strand, within RR42_RS28210 at 1686.728 kb on - strand at 1686.806 kb on - strand at 1686.962 kb on + strand, within RR42_RS28215 at 1686.963 kb on - strand, within RR42_RS28215 at 1686.963 kb on - strand, within RR42_RS28215 at 1687.115 kb on + strand, within RR42_RS28215 at 1687.116 kb on - strand, within RR42_RS28215 at 1687.247 kb on + strand, within RR42_RS28215 at 1687.248 kb on - strand, within RR42_RS28215 at 1687.547 kb on + strand, within RR42_RS28215 at 1687.547 kb on + strand, within RR42_RS28215 at 1687.547 kb on + strand, within RR42_RS28215 at 1687.599 kb on - strand, within RR42_RS28215 at 1687.674 kb on - strand at 1687.850 kb on + strand at 1687.850 kb on + strand at 1687.851 kb on - strand at 1687.851 kb on - strand at 1687.851 kb on - strand at 1687.857 kb on - strand at 1687.857 kb on - strand at 1687.881 kb on - strand at 1688.061 kb on - strand, within RR42_RS28220 at 1688.061 kb on - strand, within RR42_RS28220 at 1688.061 kb on - strand, within RR42_RS28220 at 1688.195 kb on + strand, within RR42_RS28220 at 1688.320 kb on + strand, within RR42_RS28220 at 1688.418 kb on + strand, within RR42_RS28220 at 1688.418 kb on + strand, within RR42_RS28220 at 1688.419 kb on - strand, within RR42_RS28220 at 1688.700 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM remove 1,685,891 + RR42_RS28210 0.13 -1.0 1,685,891 + RR42_RS28210 0.13 +0.4 1,685,892 - RR42_RS28210 0.13 -0.1 1,685,892 - RR42_RS28210 0.13 -0.6 1,685,892 - RR42_RS28210 0.13 +0.3 1,685,892 - RR42_RS28210 0.13 +0.5 1,686,119 + RR42_RS28210 0.37 +0.1 1,686,126 - RR42_RS28210 0.38 +0.4 1,686,126 - RR42_RS28210 0.38 +0.5 1,686,129 + RR42_RS28210 0.38 -0.2 1,686,130 - RR42_RS28210 0.39 -0.8 1,686,130 - RR42_RS28210 0.39 -1.0 1,686,130 - RR42_RS28210 0.39 -0.6 1,686,188 + RR42_RS28210 0.45 -1.6 1,686,188 + RR42_RS28210 0.45 -1.6 1,686,188 + RR42_RS28210 0.45 -0.2 1,686,189 - RR42_RS28210 0.45 -0.3 1,686,189 - RR42_RS28210 0.45 +0.4 1,686,189 - RR42_RS28210 0.45 +0.7 1,686,728 - -0.8 1,686,806 - -0.4 1,686,962 + RR42_RS28215 0.13 -0.0 1,686,963 - RR42_RS28215 0.13 -0.0 1,686,963 - RR42_RS28215 0.13 +1.0 1,687,115 + RR42_RS28215 0.29 +0.8 1,687,116 - RR42_RS28215 0.30 +0.7 1,687,247 + RR42_RS28215 0.44 +1.3 1,687,248 - RR42_RS28215 0.44 +0.2 1,687,547 + RR42_RS28215 0.77 +1.0 1,687,547 + RR42_RS28215 0.77 -0.9 1,687,547 + RR42_RS28215 0.77 +0.7 1,687,599 - RR42_RS28215 0.83 -1.1 1,687,674 - +2.9 1,687,850 + -1.1 1,687,850 + +0.1 1,687,851 - -1.3 1,687,851 - +0.7 1,687,851 - -2.8 1,687,857 - -0.9 1,687,857 - +0.8 1,687,881 - +0.4 1,688,061 - RR42_RS28220 0.31 -0.1 1,688,061 - RR42_RS28220 0.31 +0.1 1,688,061 - RR42_RS28220 0.31 -1.4 1,688,195 + RR42_RS28220 0.49 -2.0 1,688,320 + RR42_RS28220 0.66 -0.2 1,688,418 + RR42_RS28220 0.79 -0.0 1,688,418 + RR42_RS28220 0.79 +0.1 1,688,419 - RR42_RS28220 0.80 +0.6 1,688,700 + +0.7
Or see this region's nucleotide sequence