Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS27580

Experiment: R2A with Lomefloxacin 0.00075 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS27575 and RR42_RS27580 are separated by 85 nucleotidesRR42_RS27580 and RR42_RS27585 are separated by 139 nucleotides RR42_RS27575: RR42_RS27575 - arabinose transporter permease, at 1,552,097 to 1,553,254 _RS27575 RR42_RS27580: RR42_RS27580 - glutaminase, at 1,553,340 to 1,554,254 _RS27580 RR42_RS27585: RR42_RS27585 - biotin--protein ligase, at 1,554,394 to 1,555,197 _RS27585 Position (kb) 1553 1554 1555Strain fitness (log2 ratio) -2 -1 0 1 2at 1552.367 kb on + strand, within RR42_RS27575at 1552.367 kb on + strand, within RR42_RS27575at 1552.367 kb on + strand, within RR42_RS27575at 1552.367 kb on + strand, within RR42_RS27575at 1552.368 kb on - strand, within RR42_RS27575at 1552.554 kb on + strand, within RR42_RS27575at 1552.554 kb on + strand, within RR42_RS27575at 1552.554 kb on + strand, within RR42_RS27575at 1552.554 kb on + strand, within RR42_RS27575at 1552.554 kb on + strand, within RR42_RS27575at 1552.554 kb on + strand, within RR42_RS27575at 1552.554 kb on + strand, within RR42_RS27575at 1552.554 kb on + strand, within RR42_RS27575at 1552.555 kb on - strand, within RR42_RS27575at 1552.753 kb on + strand, within RR42_RS27575at 1552.754 kb on - strand, within RR42_RS27575at 1552.757 kb on + strand, within RR42_RS27575at 1552.757 kb on + strand, within RR42_RS27575at 1552.758 kb on - strand, within RR42_RS27575at 1552.766 kb on + strand, within RR42_RS27575at 1552.767 kb on - strand, within RR42_RS27575at 1552.767 kb on - strand, within RR42_RS27575at 1552.767 kb on - strand, within RR42_RS27575at 1552.924 kb on - strand, within RR42_RS27575at 1553.252 kb on + strandat 1553.252 kb on + strandat 1553.253 kb on - strandat 1553.255 kb on + strandat 1553.256 kb on - strandat 1553.304 kb on + strandat 1553.304 kb on + strandat 1553.304 kb on + strandat 1553.304 kb on + strandat 1553.305 kb on - strandat 1553.347 kb on - strandat 1553.415 kb on + strandat 1553.415 kb on + strandat 1553.415 kb on + strandat 1553.415 kb on + strandat 1553.416 kb on - strandat 1553.643 kb on + strand, within RR42_RS27580at 1553.785 kb on - strand, within RR42_RS27580at 1553.845 kb on - strand, within RR42_RS27580at 1553.901 kb on + strand, within RR42_RS27580at 1553.901 kb on + strand, within RR42_RS27580at 1553.901 kb on + strand, within RR42_RS27580at 1554.057 kb on + strand, within RR42_RS27580at 1554.057 kb on + strand, within RR42_RS27580at 1554.058 kb on - strand, within RR42_RS27580at 1554.058 kb on - strand, within RR42_RS27580at 1554.277 kb on + strandat 1554.278 kb on - strandat 1554.695 kb on + strand, within RR42_RS27585at 1554.695 kb on + strand, within RR42_RS27585at 1554.704 kb on + strand, within RR42_RS27585at 1554.705 kb on - strand, within RR42_RS27585at 1554.705 kb on - strand, within RR42_RS27585at 1554.705 kb on - strand, within RR42_RS27585at 1554.745 kb on + strand, within RR42_RS27585at 1554.745 kb on + strand, within RR42_RS27585at 1554.745 kb on + strand, within RR42_RS27585at 1554.782 kb on + strand, within RR42_RS27585at 1554.783 kb on - strand, within RR42_RS27585at 1554.905 kb on + strand, within RR42_RS27585at 1554.906 kb on - strand, within RR42_RS27585at 1554.906 kb on - strand, within RR42_RS27585at 1554.906 kb on - strand, within RR42_RS27585at 1554.908 kb on + strand, within RR42_RS27585at 1554.909 kb on - strand, within RR42_RS27585at 1555.097 kb on + strand, within RR42_RS27585at 1555.172 kb on + strandat 1555.172 kb on + strandat 1555.172 kb on + strandat 1555.172 kb on + strandat 1555.172 kb on + strandat 1555.172 kb on + strandat 1555.173 kb on - strandat 1555.173 kb on - strandat 1555.173 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM
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1,552,367 + RR42_RS27575 0.23 -0.6
1,552,367 + RR42_RS27575 0.23 +0.4
1,552,367 + RR42_RS27575 0.23 +0.2
1,552,367 + RR42_RS27575 0.23 +1.2
1,552,368 - RR42_RS27575 0.23 +0.0
1,552,554 + RR42_RS27575 0.39 +1.5
1,552,554 + RR42_RS27575 0.39 +0.8
1,552,554 + RR42_RS27575 0.39 -0.1
1,552,554 + RR42_RS27575 0.39 +0.2
1,552,554 + RR42_RS27575 0.39 -0.2
1,552,554 + RR42_RS27575 0.39 -0.1
1,552,554 + RR42_RS27575 0.39 +0.1
1,552,554 + RR42_RS27575 0.39 -0.2
1,552,555 - RR42_RS27575 0.40 +1.2
1,552,753 + RR42_RS27575 0.57 +0.1
1,552,754 - RR42_RS27575 0.57 -0.2
1,552,757 + RR42_RS27575 0.57 +0.1
1,552,757 + RR42_RS27575 0.57 +0.7
1,552,758 - RR42_RS27575 0.57 +0.6
1,552,766 + RR42_RS27575 0.58 -0.8
1,552,767 - RR42_RS27575 0.58 +0.4
1,552,767 - RR42_RS27575 0.58 +1.2
1,552,767 - RR42_RS27575 0.58 +0.1
1,552,924 - RR42_RS27575 0.71 -0.1
1,553,252 + -0.4
1,553,252 + -0.7
1,553,253 - -0.8
1,553,255 + +0.5
1,553,256 - -1.1
1,553,304 + -1.1
1,553,304 + +2.5
1,553,304 + -1.1
1,553,304 + -0.8
1,553,305 - +1.1
1,553,347 - -1.1
1,553,415 + -0.9
1,553,415 + -1.8
1,553,415 + +0.9
1,553,415 + +1.0
1,553,416 - -0.5
1,553,643 + RR42_RS27580 0.33 -0.2
1,553,785 - RR42_RS27580 0.49 +0.1
1,553,845 - RR42_RS27580 0.55 -1.5
1,553,901 + RR42_RS27580 0.61 -0.0
1,553,901 + RR42_RS27580 0.61 -1.6
1,553,901 + RR42_RS27580 0.61 -0.4
1,554,057 + RR42_RS27580 0.78 +0.9
1,554,057 + RR42_RS27580 0.78 -0.7
1,554,058 - RR42_RS27580 0.78 -0.7
1,554,058 - RR42_RS27580 0.78 -0.0
1,554,277 + -0.5
1,554,278 - -0.6
1,554,695 + RR42_RS27585 0.37 -0.4
1,554,695 + RR42_RS27585 0.37 +0.7
1,554,704 + RR42_RS27585 0.39 +0.1
1,554,705 - RR42_RS27585 0.39 +0.7
1,554,705 - RR42_RS27585 0.39 +0.6
1,554,705 - RR42_RS27585 0.39 +0.4
1,554,745 + RR42_RS27585 0.44 +1.8
1,554,745 + RR42_RS27585 0.44 +0.6
1,554,745 + RR42_RS27585 0.44 +0.5
1,554,782 + RR42_RS27585 0.48 +0.4
1,554,783 - RR42_RS27585 0.48 +0.9
1,554,905 + RR42_RS27585 0.64 +1.7
1,554,906 - RR42_RS27585 0.64 +1.1
1,554,906 - RR42_RS27585 0.64 +1.3
1,554,906 - RR42_RS27585 0.64 +0.3
1,554,908 + RR42_RS27585 0.64 +0.3
1,554,909 - RR42_RS27585 0.64 -1.3
1,555,097 + RR42_RS27585 0.87 +0.2
1,555,172 + +0.9
1,555,172 + -1.4
1,555,172 + -0.2
1,555,172 + +0.8
1,555,172 + -0.8
1,555,172 + +0.4
1,555,173 - -0.2
1,555,173 - -0.9
1,555,173 - +0.3

Or see this region's nucleotide sequence