Experiment: R2A with Lomefloxacin 0.00075 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27575 and RR42_RS27580 are separated by 85 nucleotides RR42_RS27580 and RR42_RS27585 are separated by 139 nucleotides
RR42_RS27575: RR42_RS27575 - arabinose transporter permease, at 1,552,097 to 1,553,254
_RS27575
RR42_RS27580: RR42_RS27580 - glutaminase, at 1,553,340 to 1,554,254
_RS27580
RR42_RS27585: RR42_RS27585 - biotin--protein ligase, at 1,554,394 to 1,555,197
_RS27585
Position (kb)
1553
1554
1555 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1552.367 kb on + strand, within RR42_RS27575 at 1552.367 kb on + strand, within RR42_RS27575 at 1552.367 kb on + strand, within RR42_RS27575 at 1552.367 kb on + strand, within RR42_RS27575 at 1552.368 kb on - strand, within RR42_RS27575 at 1552.554 kb on + strand, within RR42_RS27575 at 1552.554 kb on + strand, within RR42_RS27575 at 1552.554 kb on + strand, within RR42_RS27575 at 1552.554 kb on + strand, within RR42_RS27575 at 1552.554 kb on + strand, within RR42_RS27575 at 1552.554 kb on + strand, within RR42_RS27575 at 1552.554 kb on + strand, within RR42_RS27575 at 1552.554 kb on + strand, within RR42_RS27575 at 1552.555 kb on - strand, within RR42_RS27575 at 1552.753 kb on + strand, within RR42_RS27575 at 1552.754 kb on - strand, within RR42_RS27575 at 1552.757 kb on + strand, within RR42_RS27575 at 1552.757 kb on + strand, within RR42_RS27575 at 1552.758 kb on - strand, within RR42_RS27575 at 1552.766 kb on + strand, within RR42_RS27575 at 1552.767 kb on - strand, within RR42_RS27575 at 1552.767 kb on - strand, within RR42_RS27575 at 1552.767 kb on - strand, within RR42_RS27575 at 1552.924 kb on - strand, within RR42_RS27575 at 1553.252 kb on + strand at 1553.252 kb on + strand at 1553.253 kb on - strand at 1553.255 kb on + strand at 1553.256 kb on - strand at 1553.304 kb on + strand at 1553.304 kb on + strand at 1553.304 kb on + strand at 1553.304 kb on + strand at 1553.305 kb on - strand at 1553.347 kb on - strand at 1553.415 kb on + strand at 1553.415 kb on + strand at 1553.415 kb on + strand at 1553.415 kb on + strand at 1553.416 kb on - strand at 1553.643 kb on + strand, within RR42_RS27580 at 1553.785 kb on - strand, within RR42_RS27580 at 1553.845 kb on - strand, within RR42_RS27580 at 1553.901 kb on + strand, within RR42_RS27580 at 1553.901 kb on + strand, within RR42_RS27580 at 1553.901 kb on + strand, within RR42_RS27580 at 1554.057 kb on + strand, within RR42_RS27580 at 1554.057 kb on + strand, within RR42_RS27580 at 1554.058 kb on - strand, within RR42_RS27580 at 1554.058 kb on - strand, within RR42_RS27580 at 1554.277 kb on + strand at 1554.278 kb on - strand at 1554.695 kb on + strand, within RR42_RS27585 at 1554.695 kb on + strand, within RR42_RS27585 at 1554.704 kb on + strand, within RR42_RS27585 at 1554.705 kb on - strand, within RR42_RS27585 at 1554.705 kb on - strand, within RR42_RS27585 at 1554.705 kb on - strand, within RR42_RS27585 at 1554.745 kb on + strand, within RR42_RS27585 at 1554.745 kb on + strand, within RR42_RS27585 at 1554.745 kb on + strand, within RR42_RS27585 at 1554.782 kb on + strand, within RR42_RS27585 at 1554.783 kb on - strand, within RR42_RS27585 at 1554.905 kb on + strand, within RR42_RS27585 at 1554.906 kb on - strand, within RR42_RS27585 at 1554.906 kb on - strand, within RR42_RS27585 at 1554.906 kb on - strand, within RR42_RS27585 at 1554.908 kb on + strand, within RR42_RS27585 at 1554.909 kb on - strand, within RR42_RS27585 at 1555.097 kb on + strand, within RR42_RS27585 at 1555.172 kb on + strand at 1555.172 kb on + strand at 1555.172 kb on + strand at 1555.172 kb on + strand at 1555.172 kb on + strand at 1555.172 kb on + strand at 1555.173 kb on - strand at 1555.173 kb on - strand at 1555.173 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM remove 1,552,367 + RR42_RS27575 0.23 -0.6 1,552,367 + RR42_RS27575 0.23 +0.4 1,552,367 + RR42_RS27575 0.23 +0.2 1,552,367 + RR42_RS27575 0.23 +1.2 1,552,368 - RR42_RS27575 0.23 +0.0 1,552,554 + RR42_RS27575 0.39 +1.5 1,552,554 + RR42_RS27575 0.39 +0.8 1,552,554 + RR42_RS27575 0.39 -0.1 1,552,554 + RR42_RS27575 0.39 +0.2 1,552,554 + RR42_RS27575 0.39 -0.2 1,552,554 + RR42_RS27575 0.39 -0.1 1,552,554 + RR42_RS27575 0.39 +0.1 1,552,554 + RR42_RS27575 0.39 -0.2 1,552,555 - RR42_RS27575 0.40 +1.2 1,552,753 + RR42_RS27575 0.57 +0.1 1,552,754 - RR42_RS27575 0.57 -0.2 1,552,757 + RR42_RS27575 0.57 +0.1 1,552,757 + RR42_RS27575 0.57 +0.7 1,552,758 - RR42_RS27575 0.57 +0.6 1,552,766 + RR42_RS27575 0.58 -0.8 1,552,767 - RR42_RS27575 0.58 +0.4 1,552,767 - RR42_RS27575 0.58 +1.2 1,552,767 - RR42_RS27575 0.58 +0.1 1,552,924 - RR42_RS27575 0.71 -0.1 1,553,252 + -0.4 1,553,252 + -0.7 1,553,253 - -0.8 1,553,255 + +0.5 1,553,256 - -1.1 1,553,304 + -1.1 1,553,304 + +2.5 1,553,304 + -1.1 1,553,304 + -0.8 1,553,305 - +1.1 1,553,347 - -1.1 1,553,415 + -0.9 1,553,415 + -1.8 1,553,415 + +0.9 1,553,415 + +1.0 1,553,416 - -0.5 1,553,643 + RR42_RS27580 0.33 -0.2 1,553,785 - RR42_RS27580 0.49 +0.1 1,553,845 - RR42_RS27580 0.55 -1.5 1,553,901 + RR42_RS27580 0.61 -0.0 1,553,901 + RR42_RS27580 0.61 -1.6 1,553,901 + RR42_RS27580 0.61 -0.4 1,554,057 + RR42_RS27580 0.78 +0.9 1,554,057 + RR42_RS27580 0.78 -0.7 1,554,058 - RR42_RS27580 0.78 -0.7 1,554,058 - RR42_RS27580 0.78 -0.0 1,554,277 + -0.5 1,554,278 - -0.6 1,554,695 + RR42_RS27585 0.37 -0.4 1,554,695 + RR42_RS27585 0.37 +0.7 1,554,704 + RR42_RS27585 0.39 +0.1 1,554,705 - RR42_RS27585 0.39 +0.7 1,554,705 - RR42_RS27585 0.39 +0.6 1,554,705 - RR42_RS27585 0.39 +0.4 1,554,745 + RR42_RS27585 0.44 +1.8 1,554,745 + RR42_RS27585 0.44 +0.6 1,554,745 + RR42_RS27585 0.44 +0.5 1,554,782 + RR42_RS27585 0.48 +0.4 1,554,783 - RR42_RS27585 0.48 +0.9 1,554,905 + RR42_RS27585 0.64 +1.7 1,554,906 - RR42_RS27585 0.64 +1.1 1,554,906 - RR42_RS27585 0.64 +1.3 1,554,906 - RR42_RS27585 0.64 +0.3 1,554,908 + RR42_RS27585 0.64 +0.3 1,554,909 - RR42_RS27585 0.64 -1.3 1,555,097 + RR42_RS27585 0.87 +0.2 1,555,172 + +0.9 1,555,172 + -1.4 1,555,172 + -0.2 1,555,172 + +0.8 1,555,172 + -0.8 1,555,172 + +0.4 1,555,173 - -0.2 1,555,173 - -0.9 1,555,173 - +0.3
Or see this region's nucleotide sequence