Experiment: R2A with Lomefloxacin 0.00075 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS26525 and RR42_RS26530 are separated by 106 nucleotides RR42_RS26530 and RR42_RS26535 are separated by 110 nucleotides RR42_RS26535 and RR42_RS26540 overlap by 4 nucleotides
RR42_RS26525: RR42_RS26525 - IclR family transcriptional regulator, at 1,307,499 to 1,308,275
_RS26525
RR42_RS26530: RR42_RS26530 - LacI family transcriptional regulator, at 1,308,382 to 1,309,356
_RS26530
RR42_RS26535: RR42_RS26535 - diguanylate cyclase, at 1,309,467 to 1,310,150
_RS26535
RR42_RS26540: RR42_RS26540 - 3-phosphoglycerate dehydrogenase, at 1,310,147 to 1,311,082
_RS26540
Position (kb)
1308
1309
1310 Strain fitness (log2 ratio)
-2
-1
0
1 at 1307.569 kb on + strand at 1307.569 kb on + strand at 1307.569 kb on + strand at 1307.569 kb on + strand at 1307.570 kb on - strand at 1307.570 kb on - strand at 1307.703 kb on + strand, within RR42_RS26525 at 1307.747 kb on - strand, within RR42_RS26525 at 1307.770 kb on + strand, within RR42_RS26525 at 1307.923 kb on + strand, within RR42_RS26525 at 1307.924 kb on - strand, within RR42_RS26525 at 1308.007 kb on + strand, within RR42_RS26525 at 1308.008 kb on - strand, within RR42_RS26525 at 1308.073 kb on + strand, within RR42_RS26525 at 1308.119 kb on + strand, within RR42_RS26525 at 1308.119 kb on + strand, within RR42_RS26525 at 1308.119 kb on + strand, within RR42_RS26525 at 1308.119 kb on + strand, within RR42_RS26525 at 1308.120 kb on - strand, within RR42_RS26525 at 1308.643 kb on + strand, within RR42_RS26530 at 1308.643 kb on + strand, within RR42_RS26530 at 1308.644 kb on - strand, within RR42_RS26530 at 1308.695 kb on - strand, within RR42_RS26530 at 1308.709 kb on + strand, within RR42_RS26530 at 1308.728 kb on + strand, within RR42_RS26530 at 1308.730 kb on - strand, within RR42_RS26530 at 1308.790 kb on + strand, within RR42_RS26530 at 1308.934 kb on + strand, within RR42_RS26530 at 1308.934 kb on + strand, within RR42_RS26530 at 1308.934 kb on + strand, within RR42_RS26530 at 1308.935 kb on - strand, within RR42_RS26530 at 1308.935 kb on - strand, within RR42_RS26530 at 1308.988 kb on + strand, within RR42_RS26530 at 1308.988 kb on + strand, within RR42_RS26530 at 1308.988 kb on + strand, within RR42_RS26530 at 1308.989 kb on - strand, within RR42_RS26530 at 1308.989 kb on - strand, within RR42_RS26530 at 1308.989 kb on - strand, within RR42_RS26530 at 1309.020 kb on + strand, within RR42_RS26530 at 1309.260 kb on - strand at 1309.260 kb on - strand at 1309.383 kb on + strand at 1309.391 kb on + strand at 1309.392 kb on - strand at 1309.392 kb on - strand at 1309.392 kb on - strand at 1309.399 kb on + strand at 1309.399 kb on + strand at 1309.399 kb on + strand at 1309.399 kb on + strand at 1309.400 kb on - strand at 1309.400 kb on - strand at 1309.400 kb on - strand at 1309.423 kb on + strand at 1309.424 kb on - strand at 1309.424 kb on - strand at 1309.613 kb on + strand, within RR42_RS26535 at 1309.614 kb on - strand, within RR42_RS26535 at 1309.614 kb on - strand, within RR42_RS26535 at 1309.848 kb on + strand, within RR42_RS26535 at 1310.309 kb on + strand, within RR42_RS26540
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM remove 1,307,569 + -0.4 1,307,569 + -0.0 1,307,569 + +0.6 1,307,569 + +0.1 1,307,570 - +0.2 1,307,570 - +0.7 1,307,703 + RR42_RS26525 0.26 +0.5 1,307,747 - RR42_RS26525 0.32 -0.5 1,307,770 + RR42_RS26525 0.35 -1.8 1,307,923 + RR42_RS26525 0.55 +0.3 1,307,924 - RR42_RS26525 0.55 +0.1 1,308,007 + RR42_RS26525 0.65 +0.9 1,308,008 - RR42_RS26525 0.66 -0.0 1,308,073 + RR42_RS26525 0.74 +1.4 1,308,119 + RR42_RS26525 0.80 -1.4 1,308,119 + RR42_RS26525 0.80 +0.5 1,308,119 + RR42_RS26525 0.80 -0.4 1,308,119 + RR42_RS26525 0.80 +0.9 1,308,120 - RR42_RS26525 0.80 +0.5 1,308,643 + RR42_RS26530 0.27 -0.2 1,308,643 + RR42_RS26530 0.27 +0.1 1,308,644 - RR42_RS26530 0.27 -0.5 1,308,695 - RR42_RS26530 0.32 -1.7 1,308,709 + RR42_RS26530 0.34 -0.6 1,308,728 + RR42_RS26530 0.35 -0.1 1,308,730 - RR42_RS26530 0.36 +0.3 1,308,790 + RR42_RS26530 0.42 -0.1 1,308,934 + RR42_RS26530 0.57 -0.9 1,308,934 + RR42_RS26530 0.57 +0.2 1,308,934 + RR42_RS26530 0.57 +0.1 1,308,935 - RR42_RS26530 0.57 +1.7 1,308,935 - RR42_RS26530 0.57 -0.1 1,308,988 + RR42_RS26530 0.62 -0.2 1,308,988 + RR42_RS26530 0.62 -0.3 1,308,988 + RR42_RS26530 0.62 -0.6 1,308,989 - RR42_RS26530 0.62 +1.3 1,308,989 - RR42_RS26530 0.62 -0.5 1,308,989 - RR42_RS26530 0.62 +0.7 1,309,020 + RR42_RS26530 0.65 +0.2 1,309,260 - -1.7 1,309,260 - +0.4 1,309,383 + -2.0 1,309,391 + +0.3 1,309,392 - -1.2 1,309,392 - -0.6 1,309,392 - +0.0 1,309,399 + +0.6 1,309,399 + +0.6 1,309,399 + -0.3 1,309,399 + +1.6 1,309,400 - -0.8 1,309,400 - -0.6 1,309,400 - -0.8 1,309,423 + +0.1 1,309,424 - -0.7 1,309,424 - +1.1 1,309,613 + RR42_RS26535 0.21 -1.4 1,309,614 - RR42_RS26535 0.21 -0.4 1,309,614 - RR42_RS26535 0.21 -1.2 1,309,848 + RR42_RS26535 0.56 +1.0 1,310,309 + RR42_RS26540 0.17 +1.0
Or see this region's nucleotide sequence