Experiment: R2A with Lomefloxacin 0.00075 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25740 and RR42_RS25745 are separated by 299 nucleotides RR42_RS25745 and RR42_RS25750 are separated by 155 nucleotides RR42_RS25750 and RR42_RS25755 are separated by 25 nucleotides RR42_RS25755 and RR42_RS25760 are separated by 48 nucleotides
RR42_RS25740: RR42_RS25740 - hypothetical protein, at 1,116,463 to 1,117,206
_RS25740
RR42_RS25745: RR42_RS25745 - tRNA-Thr, at 1,117,506 to 1,117,580
_RS25745
RR42_RS25750: RR42_RS25750 - aromatic ring-cleaving dioxygenase, at 1,117,736 to 1,118,557
_RS25750
RR42_RS25755: RR42_RS25755 - IclR family transcriptional regulator, at 1,118,583 to 1,119,440
_RS25755
RR42_RS25760: RR42_RS25760 - salicylate hydroxylase, at 1,119,489 to 1,120,619
_RS25760
Position (kb)
1117
1118
1119 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1116.837 kb on - strand, within RR42_RS25740 at 1116.848 kb on + strand, within RR42_RS25740 at 1116.849 kb on - strand, within RR42_RS25740 at 1116.849 kb on - strand, within RR42_RS25740 at 1117.235 kb on - strand at 1117.283 kb on + strand at 1117.390 kb on + strand at 1117.479 kb on - strand at 1117.483 kb on + strand at 1117.483 kb on + strand at 1117.634 kb on + strand at 1117.634 kb on + strand at 1117.635 kb on - strand at 1117.635 kb on - strand at 1117.635 kb on - strand at 1117.753 kb on + strand at 1117.753 kb on + strand at 1117.753 kb on + strand at 1117.753 kb on + strand at 1117.754 kb on - strand at 1117.754 kb on - strand at 1117.754 kb on - strand at 1118.022 kb on + strand, within RR42_RS25750 at 1118.022 kb on + strand, within RR42_RS25750 at 1118.023 kb on - strand, within RR42_RS25750 at 1118.157 kb on + strand, within RR42_RS25750 at 1118.157 kb on + strand, within RR42_RS25750 at 1118.157 kb on + strand, within RR42_RS25750 at 1118.158 kb on - strand, within RR42_RS25750 at 1118.286 kb on + strand, within RR42_RS25750 at 1118.287 kb on - strand, within RR42_RS25750 at 1118.298 kb on + strand, within RR42_RS25750 at 1118.304 kb on + strand, within RR42_RS25750 at 1118.304 kb on + strand, within RR42_RS25750 at 1118.305 kb on - strand, within RR42_RS25750 at 1118.319 kb on + strand, within RR42_RS25750 at 1118.322 kb on + strand, within RR42_RS25750 at 1118.323 kb on - strand, within RR42_RS25750 at 1118.323 kb on - strand, within RR42_RS25750 at 1118.323 kb on - strand, within RR42_RS25750 at 1118.328 kb on + strand, within RR42_RS25750 at 1118.328 kb on + strand, within RR42_RS25750 at 1118.328 kb on + strand, within RR42_RS25750 at 1118.329 kb on - strand, within RR42_RS25750 at 1118.329 kb on - strand, within RR42_RS25750 at 1118.473 kb on - strand, within RR42_RS25750 at 1118.852 kb on - strand, within RR42_RS25755 at 1118.991 kb on - strand, within RR42_RS25755 at 1119.031 kb on + strand, within RR42_RS25755 at 1119.064 kb on + strand, within RR42_RS25755 at 1119.501 kb on + strand at 1119.502 kb on - strand at 1119.502 kb on - strand at 1119.517 kb on + strand at 1119.518 kb on - strand at 1119.518 kb on - strand at 1119.518 kb on - strand at 1119.518 kb on - strand at 1119.523 kb on + strand at 1119.523 kb on + strand at 1119.524 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM remove 1,116,837 - RR42_RS25740 0.50 -0.3 1,116,848 + RR42_RS25740 0.52 -1.0 1,116,849 - RR42_RS25740 0.52 -0.4 1,116,849 - RR42_RS25740 0.52 -0.9 1,117,235 - -1.5 1,117,283 + -0.2 1,117,390 + +0.3 1,117,479 - -0.2 1,117,483 + -0.5 1,117,483 + -0.1 1,117,634 + -2.7 1,117,634 + -0.0 1,117,635 - +0.1 1,117,635 - -0.8 1,117,635 - +0.3 1,117,753 + +2.1 1,117,753 + +0.4 1,117,753 + -0.3 1,117,753 + -0.1 1,117,754 - +0.1 1,117,754 - -0.6 1,117,754 - +0.2 1,118,022 + RR42_RS25750 0.35 -0.3 1,118,022 + RR42_RS25750 0.35 +0.1 1,118,023 - RR42_RS25750 0.35 +0.1 1,118,157 + RR42_RS25750 0.51 +0.0 1,118,157 + RR42_RS25750 0.51 -1.3 1,118,157 + RR42_RS25750 0.51 -0.5 1,118,158 - RR42_RS25750 0.51 +2.1 1,118,286 + RR42_RS25750 0.67 +0.7 1,118,287 - RR42_RS25750 0.67 +1.3 1,118,298 + RR42_RS25750 0.68 -0.1 1,118,304 + RR42_RS25750 0.69 -0.3 1,118,304 + RR42_RS25750 0.69 -1.2 1,118,305 - RR42_RS25750 0.69 -0.4 1,118,319 + RR42_RS25750 0.71 +0.5 1,118,322 + RR42_RS25750 0.71 +0.1 1,118,323 - RR42_RS25750 0.71 -0.2 1,118,323 - RR42_RS25750 0.71 +0.4 1,118,323 - RR42_RS25750 0.71 -1.1 1,118,328 + RR42_RS25750 0.72 +0.4 1,118,328 + RR42_RS25750 0.72 -0.9 1,118,328 + RR42_RS25750 0.72 -0.0 1,118,329 - RR42_RS25750 0.72 +0.7 1,118,329 - RR42_RS25750 0.72 +0.4 1,118,473 - RR42_RS25750 0.90 +1.4 1,118,852 - RR42_RS25755 0.31 -2.1 1,118,991 - RR42_RS25755 0.48 +1.1 1,119,031 + RR42_RS25755 0.52 -0.9 1,119,064 + RR42_RS25755 0.56 -1.3 1,119,501 + +0.9 1,119,502 - -0.6 1,119,502 - -2.3 1,119,517 + +1.7 1,119,518 - +0.7 1,119,518 - -0.0 1,119,518 - +0.8 1,119,518 - +0.7 1,119,523 + +0.3 1,119,523 + -0.5 1,119,524 - -0.1
Or see this region's nucleotide sequence