Experiment: R2A with Lomefloxacin 0.00075 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25455 and RR42_RS25460 are separated by 128 nucleotides RR42_RS25460 and RR42_RS25465 are separated by 5 nucleotides
RR42_RS25455: RR42_RS25455 - acetyl-CoA acetyltransferase, at 1,057,470 to 1,058,654
_RS25455
RR42_RS25460: RR42_RS25460 - IclR family transcriptional regulator, at 1,058,783 to 1,059,631
_RS25460
RR42_RS25465: RR42_RS25465 - long-chain fatty acid--CoA ligase, at 1,059,637 to 1,061,289
_RS25465
Position (kb)
1058
1059
1060 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1057.974 kb on + strand, within RR42_RS25455 at 1058.589 kb on + strand at 1058.784 kb on + strand at 1058.785 kb on - strand at 1058.880 kb on - strand, within RR42_RS25460 at 1059.119 kb on + strand, within RR42_RS25460 at 1059.184 kb on + strand, within RR42_RS25460 at 1059.217 kb on + strand, within RR42_RS25460 at 1059.217 kb on + strand, within RR42_RS25460 at 1059.219 kb on + strand, within RR42_RS25460 at 1059.220 kb on - strand, within RR42_RS25460 at 1059.220 kb on - strand, within RR42_RS25460 at 1059.220 kb on - strand, within RR42_RS25460 at 1059.418 kb on + strand, within RR42_RS25460 at 1059.418 kb on + strand, within RR42_RS25460 at 1059.419 kb on - strand, within RR42_RS25460 at 1059.419 kb on - strand, within RR42_RS25460 at 1059.420 kb on + strand, within RR42_RS25460 at 1059.421 kb on - strand, within RR42_RS25460 at 1059.663 kb on - strand at 1059.676 kb on - strand at 1059.689 kb on + strand at 1059.689 kb on + strand at 1059.730 kb on + strand at 1059.730 kb on + strand at 1059.749 kb on + strand at 1059.750 kb on - strand at 1059.750 kb on - strand at 1059.750 kb on - strand at 1059.777 kb on - strand at 1059.908 kb on + strand, within RR42_RS25465 at 1059.908 kb on + strand, within RR42_RS25465 at 1059.908 kb on + strand, within RR42_RS25465 at 1059.909 kb on - strand, within RR42_RS25465 at 1060.023 kb on + strand, within RR42_RS25465 at 1060.024 kb on - strand, within RR42_RS25465 at 1060.030 kb on - strand, within RR42_RS25465 at 1060.038 kb on + strand, within RR42_RS25465 at 1060.038 kb on + strand, within RR42_RS25465 at 1060.039 kb on - strand, within RR42_RS25465 at 1060.137 kb on + strand, within RR42_RS25465 at 1060.137 kb on + strand, within RR42_RS25465 at 1060.183 kb on + strand, within RR42_RS25465 at 1060.183 kb on + strand, within RR42_RS25465 at 1060.184 kb on - strand, within RR42_RS25465 at 1060.184 kb on - strand, within RR42_RS25465 at 1060.184 kb on - strand, within RR42_RS25465 at 1060.184 kb on - strand, within RR42_RS25465 at 1060.308 kb on + strand, within RR42_RS25465 at 1060.308 kb on + strand, within RR42_RS25465 at 1060.309 kb on - strand, within RR42_RS25465 at 1060.518 kb on + strand, within RR42_RS25465 at 1060.518 kb on + strand, within RR42_RS25465 at 1060.519 kb on - strand, within RR42_RS25465 at 1060.519 kb on - strand, within RR42_RS25465 at 1060.519 kb on - strand, within RR42_RS25465 at 1060.533 kb on - strand, within RR42_RS25465 at 1060.566 kb on - strand, within RR42_RS25465 at 1060.613 kb on + strand, within RR42_RS25465
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM remove 1,057,974 + RR42_RS25455 0.43 -0.6 1,058,589 + +1.4 1,058,784 + +0.5 1,058,785 - -0.2 1,058,880 - RR42_RS25460 0.11 +0.4 1,059,119 + RR42_RS25460 0.40 -0.0 1,059,184 + RR42_RS25460 0.47 +1.8 1,059,217 + RR42_RS25460 0.51 -0.1 1,059,217 + RR42_RS25460 0.51 +1.0 1,059,219 + RR42_RS25460 0.51 -0.3 1,059,220 - RR42_RS25460 0.51 +0.4 1,059,220 - RR42_RS25460 0.51 +0.1 1,059,220 - RR42_RS25460 0.51 -0.2 1,059,418 + RR42_RS25460 0.75 +0.7 1,059,418 + RR42_RS25460 0.75 +0.2 1,059,419 - RR42_RS25460 0.75 +0.9 1,059,419 - RR42_RS25460 0.75 -0.6 1,059,420 + RR42_RS25460 0.75 +0.1 1,059,421 - RR42_RS25460 0.75 -2.0 1,059,663 - +0.5 1,059,676 - -0.0 1,059,689 + +0.5 1,059,689 + +0.5 1,059,730 + -0.3 1,059,730 + -0.1 1,059,749 + +0.3 1,059,750 - +0.5 1,059,750 - -0.8 1,059,750 - -0.2 1,059,777 - +0.7 1,059,908 + RR42_RS25465 0.16 +0.3 1,059,908 + RR42_RS25465 0.16 -0.4 1,059,908 + RR42_RS25465 0.16 -0.2 1,059,909 - RR42_RS25465 0.16 +0.2 1,060,023 + RR42_RS25465 0.23 +0.5 1,060,024 - RR42_RS25465 0.23 -0.4 1,060,030 - RR42_RS25465 0.24 -0.6 1,060,038 + RR42_RS25465 0.24 +0.1 1,060,038 + RR42_RS25465 0.24 -0.0 1,060,039 - RR42_RS25465 0.24 +0.3 1,060,137 + RR42_RS25465 0.30 -0.4 1,060,137 + RR42_RS25465 0.30 -0.3 1,060,183 + RR42_RS25465 0.33 +0.4 1,060,183 + RR42_RS25465 0.33 +0.8 1,060,184 - RR42_RS25465 0.33 +0.8 1,060,184 - RR42_RS25465 0.33 +0.7 1,060,184 - RR42_RS25465 0.33 +0.6 1,060,184 - RR42_RS25465 0.33 -0.3 1,060,308 + RR42_RS25465 0.41 +1.0 1,060,308 + RR42_RS25465 0.41 +0.6 1,060,309 - RR42_RS25465 0.41 +2.0 1,060,518 + RR42_RS25465 0.53 -0.1 1,060,518 + RR42_RS25465 0.53 -0.5 1,060,519 - RR42_RS25465 0.53 -1.0 1,060,519 - RR42_RS25465 0.53 -2.5 1,060,519 - RR42_RS25465 0.53 -1.1 1,060,533 - RR42_RS25465 0.54 +1.1 1,060,566 - RR42_RS25465 0.56 +0.4 1,060,613 + RR42_RS25465 0.59 +0.2
Or see this region's nucleotide sequence