Experiment: R2A with Lomefloxacin 0.00075 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS24775 and RR42_RS24780 are separated by 160 nucleotides RR42_RS24780 and RR42_RS24785 are separated by 74 nucleotides
RR42_RS24775: RR42_RS24775 - transcriptional regulator, at 896,705 to 897,655
_RS24775
RR42_RS24780: RR42_RS24780 - alpha/beta hydrolase, at 897,816 to 898,736
_RS24780
RR42_RS24785: RR42_RS24785 - NAD-dependent dehydratase, at 898,811 to 899,458
_RS24785
Position (kb) 897
898
899 Strain fitness (log2 ratio) -2
-1
0
1
2 at 896.827 kb on - strand, within RR42_RS24775 at 896.827 kb on - strand, within RR42_RS24775 at 896.830 kb on + strand, within RR42_RS24775 at 896.830 kb on + strand, within RR42_RS24775 at 896.830 kb on + strand, within RR42_RS24775 at 896.830 kb on + strand, within RR42_RS24775 at 896.831 kb on - strand, within RR42_RS24775 at 896.831 kb on - strand, within RR42_RS24775 at 896.832 kb on + strand, within RR42_RS24775 at 896.832 kb on + strand, within RR42_RS24775 at 896.832 kb on + strand, within RR42_RS24775 at 896.833 kb on - strand, within RR42_RS24775 at 896.833 kb on - strand, within RR42_RS24775 at 896.917 kb on + strand, within RR42_RS24775 at 896.943 kb on - strand, within RR42_RS24775 at 896.943 kb on - strand, within RR42_RS24775 at 896.977 kb on + strand, within RR42_RS24775 at 897.136 kb on - strand, within RR42_RS24775 at 897.358 kb on + strand, within RR42_RS24775 at 897.358 kb on + strand, within RR42_RS24775 at 897.359 kb on - strand, within RR42_RS24775 at 897.359 kb on - strand, within RR42_RS24775 at 897.501 kb on + strand, within RR42_RS24775 at 897.502 kb on - strand, within RR42_RS24775 at 897.675 kb on + strand at 897.702 kb on + strand at 897.706 kb on + strand at 897.706 kb on + strand at 897.720 kb on + strand at 897.729 kb on + strand at 897.729 kb on + strand at 897.729 kb on + strand at 897.730 kb on - strand at 897.730 kb on - strand at 897.730 kb on - strand at 897.730 kb on - strand at 897.843 kb on + strand at 897.843 kb on + strand at 897.844 kb on - strand at 897.844 kb on - strand at 897.868 kb on - strand at 898.098 kb on + strand, within RR42_RS24780 at 898.099 kb on - strand, within RR42_RS24780 at 898.116 kb on + strand, within RR42_RS24780 at 898.218 kb on - strand, within RR42_RS24780 at 898.218 kb on - strand, within RR42_RS24780 at 898.233 kb on + strand, within RR42_RS24780 at 898.233 kb on + strand, within RR42_RS24780 at 898.234 kb on - strand, within RR42_RS24780 at 898.402 kb on + strand, within RR42_RS24780 at 898.403 kb on - strand, within RR42_RS24780 at 898.660 kb on - strand at 898.660 kb on - strand at 898.719 kb on + strand at 898.719 kb on + strand at 898.720 kb on - strand at 898.720 kb on - strand at 898.720 kb on - strand at 898.720 kb on - strand at 898.735 kb on - strand at 898.833 kb on + strand at 898.833 kb on + strand at 898.834 kb on - strand at 898.834 kb on - strand at 898.875 kb on + strand at 898.875 kb on + strand at 899.011 kb on + strand, within RR42_RS24785 at 899.012 kb on - strand, within RR42_RS24785 at 899.033 kb on + strand, within RR42_RS24785 at 899.193 kb on - strand, within RR42_RS24785 at 899.193 kb on - strand, within RR42_RS24785 at 899.246 kb on + strand, within RR42_RS24785 at 899.246 kb on + strand, within RR42_RS24785 at 899.295 kb on - strand, within RR42_RS24785 at 899.429 kb on - strand at 899.472 kb on + strand at 899.472 kb on + strand at 899.472 kb on + strand at 899.473 kb on - strand at 899.536 kb on + strand at 899.536 kb on + strand at 899.536 kb on + strand at 899.536 kb on + strand at 899.537 kb on - strand at 899.559 kb on + strand at 899.559 kb on + strand at 899.559 kb on + strand at 899.560 kb on - strand at 899.560 kb on - strand at 899.560 kb on - strand at 899.560 kb on - strand at 899.560 kb on - strand at 899.560 kb on - strand at 899.655 kb on + strand at 899.655 kb on + strand at 899.656 kb on - strand at 899.656 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM remove 896,827 - RR42_RS24775 0.13 -0.1 896,827 - RR42_RS24775 0.13 +0.0 896,830 + RR42_RS24775 0.13 -0.0 896,830 + RR42_RS24775 0.13 +0.1 896,830 + RR42_RS24775 0.13 +1.2 896,830 + RR42_RS24775 0.13 -1.2 896,831 - RR42_RS24775 0.13 +0.1 896,831 - RR42_RS24775 0.13 +0.9 896,832 + RR42_RS24775 0.13 -0.0 896,832 + RR42_RS24775 0.13 +0.1 896,832 + RR42_RS24775 0.13 -2.2 896,833 - RR42_RS24775 0.13 +0.2 896,833 - RR42_RS24775 0.13 +0.1 896,917 + RR42_RS24775 0.22 +0.4 896,943 - RR42_RS24775 0.25 -0.4 896,943 - RR42_RS24775 0.25 -0.0 896,977 + RR42_RS24775 0.29 +0.3 897,136 - RR42_RS24775 0.45 +1.2 897,358 + RR42_RS24775 0.69 +0.7 897,358 + RR42_RS24775 0.69 -1.1 897,359 - RR42_RS24775 0.69 -1.0 897,359 - RR42_RS24775 0.69 +0.6 897,501 + RR42_RS24775 0.84 +0.3 897,502 - RR42_RS24775 0.84 +0.3 897,675 + -0.2 897,702 + +0.0 897,706 + -0.7 897,706 + -0.2 897,720 + +0.3 897,729 + +0.2 897,729 + -0.3 897,729 + -0.5 897,730 - -0.2 897,730 - -0.2 897,730 - +0.1 897,730 - +0.5 897,843 + +0.5 897,843 + +0.6 897,844 - -0.2 897,844 - +0.2 897,868 - -0.4 898,098 + RR42_RS24780 0.31 +0.9 898,099 - RR42_RS24780 0.31 +0.8 898,116 + RR42_RS24780 0.33 -0.0 898,218 - RR42_RS24780 0.44 -0.3 898,218 - RR42_RS24780 0.44 -0.4 898,233 + RR42_RS24780 0.45 +1.8 898,233 + RR42_RS24780 0.45 +1.9 898,234 - RR42_RS24780 0.45 +0.4 898,402 + RR42_RS24780 0.64 -0.4 898,403 - RR42_RS24780 0.64 -0.3 898,660 - -0.1 898,660 - -0.2 898,719 + -1.1 898,719 + +0.4 898,720 - +0.7 898,720 - +0.2 898,720 - -0.0 898,720 - -0.6 898,735 - -0.5 898,833 + -0.1 898,833 + +0.8 898,834 - +0.1 898,834 - -0.6 898,875 + -0.5 898,875 + +2.3 899,011 + RR42_RS24785 0.31 -0.3 899,012 - RR42_RS24785 0.31 +0.2 899,033 + RR42_RS24785 0.34 -0.2 899,193 - RR42_RS24785 0.59 +0.0 899,193 - RR42_RS24785 0.59 -0.4 899,246 + RR42_RS24785 0.67 -1.5 899,246 + RR42_RS24785 0.67 -0.8 899,295 - RR42_RS24785 0.75 -1.3 899,429 - -0.9 899,472 + -0.3 899,472 + -0.4 899,472 + +0.4 899,473 - +0.5 899,536 + -0.1 899,536 + +0.7 899,536 + +0.2 899,536 + +0.3 899,537 - -0.3 899,559 + +0.4 899,559 + +1.3 899,559 + +0.0 899,560 - +1.7 899,560 - +0.4 899,560 - +0.5 899,560 - +1.0 899,560 - -1.9 899,560 - -0.2 899,655 + -1.7 899,655 + +0.4 899,656 - -0.8 899,656 - +0.1
Or see this region's nucleotide sequence