Experiment: R2A with Lomefloxacin 0.00075 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23745 and RR42_RS23750 are separated by 66 nucleotides RR42_RS23750 and RR42_RS23755 are separated by 170 nucleotides
RR42_RS23745: RR42_RS23745 - phenylacetic acid degradation protein, at 651,275 to 652,363
_RS23745
RR42_RS23750: RR42_RS23750 - aldehyde dehydrogenase, at 652,430 to 654,112
_RS23750
RR42_RS23755: RR42_RS23755 - phenylacetate--CoA ligase, at 654,283 to 655,608
_RS23755
Position (kb) 652
653
654
655 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 651.636 kb on - strand, within RR42_RS23745 at 652.136 kb on + strand, within RR42_RS23745 at 652.137 kb on - strand, within RR42_RS23745 at 652.190 kb on + strand, within RR42_RS23745 at 652.190 kb on + strand, within RR42_RS23745 at 652.191 kb on - strand, within RR42_RS23745 at 652.191 kb on - strand, within RR42_RS23745 at 652.349 kb on + strand at 652.502 kb on + strand at 652.899 kb on + strand, within RR42_RS23750 at 652.899 kb on + strand, within RR42_RS23750 at 652.913 kb on + strand, within RR42_RS23750 at 652.914 kb on - strand, within RR42_RS23750 at 652.952 kb on + strand, within RR42_RS23750 at 653.070 kb on - strand, within RR42_RS23750 at 653.348 kb on + strand, within RR42_RS23750 at 653.349 kb on - strand, within RR42_RS23750 at 653.349 kb on - strand, within RR42_RS23750 at 653.349 kb on - strand, within RR42_RS23750 at 653.477 kb on + strand, within RR42_RS23750 at 653.477 kb on + strand, within RR42_RS23750 at 653.477 kb on + strand, within RR42_RS23750 at 653.477 kb on + strand, within RR42_RS23750 at 653.477 kb on + strand, within RR42_RS23750 at 653.477 kb on + strand, within RR42_RS23750 at 653.478 kb on - strand, within RR42_RS23750 at 653.478 kb on - strand, within RR42_RS23750 at 653.880 kb on - strand, within RR42_RS23750 at 653.881 kb on + strand, within RR42_RS23750 at 653.881 kb on + strand, within RR42_RS23750 at 653.881 kb on + strand, within RR42_RS23750 at 654.110 kb on + strand at 654.110 kb on + strand at 654.111 kb on - strand at 654.142 kb on + strand at 654.143 kb on - strand at 654.143 kb on - strand at 654.330 kb on - strand at 654.394 kb on + strand at 654.394 kb on + strand at 654.394 kb on + strand at 654.394 kb on + strand at 654.394 kb on + strand at 654.395 kb on - strand at 654.395 kb on - strand at 654.395 kb on - strand at 654.395 kb on - strand at 654.395 kb on - strand at 654.395 kb on - strand at 654.395 kb on - strand at 654.490 kb on - strand, within RR42_RS23755 at 654.490 kb on - strand, within RR42_RS23755 at 654.517 kb on + strand, within RR42_RS23755 at 654.518 kb on - strand, within RR42_RS23755 at 654.803 kb on + strand, within RR42_RS23755 at 654.804 kb on - strand, within RR42_RS23755 at 654.804 kb on - strand, within RR42_RS23755 at 654.804 kb on - strand, within RR42_RS23755 at 654.853 kb on + strand, within RR42_RS23755 at 654.853 kb on + strand, within RR42_RS23755 at 654.853 kb on + strand, within RR42_RS23755 at 654.853 kb on + strand, within RR42_RS23755 at 654.854 kb on - strand, within RR42_RS23755 at 654.854 kb on - strand, within RR42_RS23755 at 654.861 kb on + strand, within RR42_RS23755 at 654.862 kb on - strand, within RR42_RS23755 at 655.003 kb on + strand, within RR42_RS23755 at 655.004 kb on - strand, within RR42_RS23755 at 655.004 kb on - strand, within RR42_RS23755 at 655.004 kb on - strand, within RR42_RS23755
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM remove 651,636 - RR42_RS23745 0.33 +0.7 652,136 + RR42_RS23745 0.79 -0.2 652,137 - RR42_RS23745 0.79 -1.0 652,190 + RR42_RS23745 0.84 -0.5 652,190 + RR42_RS23745 0.84 -0.5 652,191 - RR42_RS23745 0.84 -0.1 652,191 - RR42_RS23745 0.84 -0.7 652,349 + +1.0 652,502 + +0.2 652,899 + RR42_RS23750 0.28 -0.3 652,899 + RR42_RS23750 0.28 -0.7 652,913 + RR42_RS23750 0.29 -1.2 652,914 - RR42_RS23750 0.29 -0.1 652,952 + RR42_RS23750 0.31 -1.1 653,070 - RR42_RS23750 0.38 -0.4 653,348 + RR42_RS23750 0.55 -0.2 653,349 - RR42_RS23750 0.55 +0.5 653,349 - RR42_RS23750 0.55 +0.6 653,349 - RR42_RS23750 0.55 +0.1 653,477 + RR42_RS23750 0.62 -1.6 653,477 + RR42_RS23750 0.62 -1.5 653,477 + RR42_RS23750 0.62 +0.5 653,477 + RR42_RS23750 0.62 +0.7 653,477 + RR42_RS23750 0.62 -0.6 653,477 + RR42_RS23750 0.62 +0.2 653,478 - RR42_RS23750 0.62 +0.6 653,478 - RR42_RS23750 0.62 -0.4 653,880 - RR42_RS23750 0.86 -1.5 653,881 + RR42_RS23750 0.86 -2.0 653,881 + RR42_RS23750 0.86 +0.3 653,881 + RR42_RS23750 0.86 +0.2 654,110 + +0.5 654,110 + -1.1 654,111 - -0.5 654,142 + -2.9 654,143 - -0.8 654,143 - -0.9 654,330 - -1.0 654,394 + +0.2 654,394 + +0.3 654,394 + -0.9 654,394 + +0.5 654,394 + +0.0 654,395 - -1.1 654,395 - +0.4 654,395 - -1.0 654,395 - -0.9 654,395 - +0.2 654,395 - -0.1 654,395 - +1.1 654,490 - RR42_RS23755 0.16 +2.2 654,490 - RR42_RS23755 0.16 -0.1 654,517 + RR42_RS23755 0.18 +1.2 654,518 - RR42_RS23755 0.18 +0.2 654,803 + RR42_RS23755 0.39 -0.4 654,804 - RR42_RS23755 0.39 -0.1 654,804 - RR42_RS23755 0.39 +0.4 654,804 - RR42_RS23755 0.39 +0.4 654,853 + RR42_RS23755 0.43 -1.1 654,853 + RR42_RS23755 0.43 -0.8 654,853 + RR42_RS23755 0.43 +0.5 654,853 + RR42_RS23755 0.43 +0.4 654,854 - RR42_RS23755 0.43 -0.8 654,854 - RR42_RS23755 0.43 -0.3 654,861 + RR42_RS23755 0.44 -0.8 654,862 - RR42_RS23755 0.44 -0.0 655,003 + RR42_RS23755 0.54 -0.3 655,004 - RR42_RS23755 0.54 -0.8 655,004 - RR42_RS23755 0.54 -0.3 655,004 - RR42_RS23755 0.54 -0.4
Or see this region's nucleotide sequence