Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS22960

Experiment: R2A with Lomefloxacin 0.00075 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS22955 and RR42_RS22960 are separated by 168 nucleotidesRR42_RS22960 and RR42_RS22965 are separated by 45 nucleotidesRR42_RS22965 and RR42_RS22970 overlap by 4 nucleotides RR42_RS22955: RR42_RS22955 - isocitrate dehydrogenase, at 483,676 to 484,707 _RS22955 RR42_RS22960: RR42_RS22960 - alpha/beta hydrolase, at 484,876 to 485,757 _RS22960 RR42_RS22965: RR42_RS22965 - 3-oxoadipate CoA-transferase, at 485,803 to 486,438 _RS22965 RR42_RS22970: RR42_RS22970 - 3-oxoadipate CoA-transferase, at 486,435 to 487,127 _RS22970 Position (kb) 484 485 486Strain fitness (log2 ratio) -2 -1 0 1 2 3at 483.977 kb on + strand, within RR42_RS22955at 483.977 kb on + strand, within RR42_RS22955at 483.977 kb on + strand, within RR42_RS22955at 484.017 kb on + strand, within RR42_RS22955at 484.057 kb on + strand, within RR42_RS22955at 484.058 kb on - strand, within RR42_RS22955at 484.073 kb on - strand, within RR42_RS22955at 484.073 kb on - strand, within RR42_RS22955at 484.075 kb on + strand, within RR42_RS22955at 484.076 kb on - strand, within RR42_RS22955at 484.128 kb on + strand, within RR42_RS22955at 484.171 kb on + strand, within RR42_RS22955at 484.171 kb on + strand, within RR42_RS22955at 484.280 kb on - strand, within RR42_RS22955at 484.371 kb on + strand, within RR42_RS22955at 484.895 kb on - strandat 484.895 kb on - strandat 485.054 kb on - strand, within RR42_RS22960at 485.062 kb on + strand, within RR42_RS22960at 485.062 kb on + strand, within RR42_RS22960at 485.251 kb on + strand, within RR42_RS22960at 485.252 kb on - strand, within RR42_RS22960at 485.252 kb on - strand, within RR42_RS22960at 485.285 kb on + strand, within RR42_RS22960at 485.286 kb on - strand, within RR42_RS22960at 485.428 kb on + strand, within RR42_RS22960at 485.428 kb on + strand, within RR42_RS22960at 485.428 kb on + strand, within RR42_RS22960at 485.428 kb on + strand, within RR42_RS22960at 485.428 kb on + strand, within RR42_RS22960at 485.429 kb on - strand, within RR42_RS22960at 485.429 kb on - strand, within RR42_RS22960at 485.429 kb on - strand, within RR42_RS22960at 485.429 kb on - strand, within RR42_RS22960at 485.429 kb on - strand, within RR42_RS22960at 485.517 kb on + strand, within RR42_RS22960at 485.518 kb on - strand, within RR42_RS22960at 485.551 kb on + strand, within RR42_RS22960at 485.551 kb on + strand, within RR42_RS22960at 485.551 kb on + strand, within RR42_RS22960at 485.551 kb on + strand, within RR42_RS22960at 485.552 kb on - strand, within RR42_RS22960at 485.552 kb on - strand, within RR42_RS22960at 485.552 kb on - strand, within RR42_RS22960at 485.810 kb on - strandat 485.916 kb on + strand, within RR42_RS22965at 485.919 kb on - strand, within RR42_RS22965at 485.919 kb on - strand, within RR42_RS22965at 486.122 kb on + strand, within RR42_RS22965at 486.300 kb on + strand, within RR42_RS22965at 486.374 kb on + strand, within RR42_RS22965at 486.619 kb on + strand, within RR42_RS22970

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM
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483,977 + RR42_RS22955 0.29 +0.2
483,977 + RR42_RS22955 0.29 +0.6
483,977 + RR42_RS22955 0.29 -1.0
484,017 + RR42_RS22955 0.33 +0.3
484,057 + RR42_RS22955 0.37 -0.3
484,058 - RR42_RS22955 0.37 -0.7
484,073 - RR42_RS22955 0.38 +0.0
484,073 - RR42_RS22955 0.38 -0.5
484,075 + RR42_RS22955 0.39 -1.9
484,076 - RR42_RS22955 0.39 -2.7
484,128 + RR42_RS22955 0.44 -1.2
484,171 + RR42_RS22955 0.48 -0.4
484,171 + RR42_RS22955 0.48 +0.1
484,280 - RR42_RS22955 0.59 -1.0
484,371 + RR42_RS22955 0.67 +0.6
484,895 - +0.8
484,895 - -1.1
485,054 - RR42_RS22960 0.20 -1.4
485,062 + RR42_RS22960 0.21 +2.9
485,062 + RR42_RS22960 0.21 -0.6
485,251 + RR42_RS22960 0.43 -1.2
485,252 - RR42_RS22960 0.43 -1.6
485,252 - RR42_RS22960 0.43 -0.8
485,285 + RR42_RS22960 0.46 -0.0
485,286 - RR42_RS22960 0.46 -0.5
485,428 + RR42_RS22960 0.63 +0.1
485,428 + RR42_RS22960 0.63 -2.7
485,428 + RR42_RS22960 0.63 -0.4
485,428 + RR42_RS22960 0.63 +0.3
485,428 + RR42_RS22960 0.63 -0.2
485,429 - RR42_RS22960 0.63 +3.1
485,429 - RR42_RS22960 0.63 -0.0
485,429 - RR42_RS22960 0.63 +0.4
485,429 - RR42_RS22960 0.63 -0.1
485,429 - RR42_RS22960 0.63 -0.3
485,517 + RR42_RS22960 0.73 +0.4
485,518 - RR42_RS22960 0.73 -1.7
485,551 + RR42_RS22960 0.77 -1.0
485,551 + RR42_RS22960 0.77 -0.0
485,551 + RR42_RS22960 0.77 -1.1
485,551 + RR42_RS22960 0.77 -0.3
485,552 - RR42_RS22960 0.77 -0.7
485,552 - RR42_RS22960 0.77 +0.3
485,552 - RR42_RS22960 0.77 -0.4
485,810 - -0.1
485,916 + RR42_RS22965 0.18 -0.6
485,919 - RR42_RS22965 0.18 -0.7
485,919 - RR42_RS22965 0.18 -0.9
486,122 + RR42_RS22965 0.50 +0.8
486,300 + RR42_RS22965 0.78 -0.7
486,374 + RR42_RS22965 0.90 +0.3
486,619 + RR42_RS22970 0.27 -0.9

Or see this region's nucleotide sequence