Experiment: R2A with Lomefloxacin 0.00075 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22725 and RR42_RS22730 are separated by 480 nucleotides RR42_RS22730 and RR42_RS22735 are separated by 20 nucleotides RR42_RS22735 and RR42_RS22740 are separated by 121 nucleotides
RR42_RS22725: RR42_RS22725 - phosphatidylethanolamine-binding protein, at 432,778 to 433,332
_RS22725
RR42_RS22730: RR42_RS22730 - pyridine nucleotide-disulfide oxidoreductase, at 433,813 to 435,018
_RS22730
RR42_RS22735: RR42_RS22735 - transcriptional regulator, at 435,039 to 435,578
_RS22735
RR42_RS22740: RR42_RS22740 - oxidoreductase, at 435,700 to 436,494
_RS22740
Position (kb) 433
434
435
436 Strain fitness (log2 ratio) -2
-1
0
1
2 at 432.847 kb on + strand, within RR42_RS22725 at 432.847 kb on + strand, within RR42_RS22725 at 432.847 kb on + strand, within RR42_RS22725 at 432.847 kb on + strand, within RR42_RS22725 at 432.910 kb on + strand, within RR42_RS22725 at 432.910 kb on + strand, within RR42_RS22725 at 432.921 kb on + strand, within RR42_RS22725 at 432.921 kb on + strand, within RR42_RS22725 at 432.921 kb on + strand, within RR42_RS22725 at 432.921 kb on + strand, within RR42_RS22725 at 432.922 kb on - strand, within RR42_RS22725 at 432.922 kb on - strand, within RR42_RS22725 at 432.922 kb on - strand, within RR42_RS22725 at 433.034 kb on + strand, within RR42_RS22725 at 433.039 kb on + strand, within RR42_RS22725 at 433.057 kb on + strand, within RR42_RS22725 at 433.059 kb on + strand, within RR42_RS22725 at 433.060 kb on - strand, within RR42_RS22725 at 433.139 kb on + strand, within RR42_RS22725 at 433.198 kb on + strand, within RR42_RS22725 at 433.198 kb on + strand, within RR42_RS22725 at 433.199 kb on - strand, within RR42_RS22725 at 433.199 kb on - strand, within RR42_RS22725 at 433.322 kb on - strand at 433.342 kb on + strand at 433.343 kb on - strand at 433.433 kb on + strand at 433.434 kb on - strand at 433.793 kb on - strand at 433.819 kb on - strand at 433.825 kb on - strand at 433.963 kb on + strand, within RR42_RS22730 at 434.116 kb on + strand, within RR42_RS22730 at 434.117 kb on - strand, within RR42_RS22730 at 434.117 kb on - strand, within RR42_RS22730 at 434.117 kb on - strand, within RR42_RS22730 at 434.117 kb on - strand, within RR42_RS22730 at 434.136 kb on + strand, within RR42_RS22730 at 434.233 kb on - strand, within RR42_RS22730 at 434.233 kb on - strand, within RR42_RS22730 at 434.274 kb on + strand, within RR42_RS22730 at 434.275 kb on - strand, within RR42_RS22730 at 434.693 kb on - strand, within RR42_RS22730 at 434.693 kb on - strand, within RR42_RS22730 at 434.719 kb on + strand, within RR42_RS22730 at 434.719 kb on + strand, within RR42_RS22730 at 434.719 kb on + strand, within RR42_RS22730 at 434.720 kb on - strand, within RR42_RS22730 at 434.720 kb on - strand, within RR42_RS22730 at 435.011 kb on + strand at 435.016 kb on + strand at 435.105 kb on + strand, within RR42_RS22735 at 435.105 kb on + strand, within RR42_RS22735 at 435.106 kb on - strand, within RR42_RS22735 at 435.106 kb on - strand, within RR42_RS22735 at 435.452 kb on - strand, within RR42_RS22735 at 435.495 kb on - strand, within RR42_RS22735 at 435.605 kb on - strand at 435.615 kb on - strand at 435.617 kb on - strand at 435.712 kb on - strand at 435.780 kb on - strand, within RR42_RS22740 at 435.780 kb on - strand, within RR42_RS22740
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM remove 432,847 + RR42_RS22725 0.12 +1.0 432,847 + RR42_RS22725 0.12 -0.2 432,847 + RR42_RS22725 0.12 -0.0 432,847 + RR42_RS22725 0.12 -0.8 432,910 + RR42_RS22725 0.24 +1.9 432,910 + RR42_RS22725 0.24 +0.3 432,921 + RR42_RS22725 0.26 +0.2 432,921 + RR42_RS22725 0.26 +0.6 432,921 + RR42_RS22725 0.26 -2.0 432,921 + RR42_RS22725 0.26 -0.9 432,922 - RR42_RS22725 0.26 -1.0 432,922 - RR42_RS22725 0.26 +0.0 432,922 - RR42_RS22725 0.26 -0.6 433,034 + RR42_RS22725 0.46 +0.4 433,039 + RR42_RS22725 0.47 +0.1 433,057 + RR42_RS22725 0.50 -0.2 433,059 + RR42_RS22725 0.51 +0.6 433,060 - RR42_RS22725 0.51 +0.1 433,139 + RR42_RS22725 0.65 +0.4 433,198 + RR42_RS22725 0.76 +0.8 433,198 + RR42_RS22725 0.76 -0.0 433,199 - RR42_RS22725 0.76 +0.2 433,199 - RR42_RS22725 0.76 -0.2 433,322 - -0.8 433,342 + -0.6 433,343 - -0.5 433,433 + -0.2 433,434 - +0.7 433,793 - +1.6 433,819 - +1.1 433,825 - -1.1 433,963 + RR42_RS22730 0.12 +1.0 434,116 + RR42_RS22730 0.25 -0.4 434,117 - RR42_RS22730 0.25 -1.9 434,117 - RR42_RS22730 0.25 +0.9 434,117 - RR42_RS22730 0.25 +0.4 434,117 - RR42_RS22730 0.25 +0.6 434,136 + RR42_RS22730 0.27 -0.1 434,233 - RR42_RS22730 0.35 +0.2 434,233 - RR42_RS22730 0.35 +2.3 434,274 + RR42_RS22730 0.38 -0.3 434,275 - RR42_RS22730 0.38 +0.3 434,693 - RR42_RS22730 0.73 -0.1 434,693 - RR42_RS22730 0.73 -0.1 434,719 + RR42_RS22730 0.75 -0.1 434,719 + RR42_RS22730 0.75 -0.2 434,719 + RR42_RS22730 0.75 -0.0 434,720 - RR42_RS22730 0.75 +1.2 434,720 - RR42_RS22730 0.75 +0.6 435,011 + +0.7 435,016 + -0.5 435,105 + RR42_RS22735 0.12 -1.7 435,105 + RR42_RS22735 0.12 +0.6 435,106 - RR42_RS22735 0.12 +0.0 435,106 - RR42_RS22735 0.12 -0.4 435,452 - RR42_RS22735 0.76 +0.1 435,495 - RR42_RS22735 0.84 +1.1 435,605 - -0.9 435,615 - -1.2 435,617 - -0.3 435,712 - +0.5 435,780 - RR42_RS22740 0.10 -0.4 435,780 - RR42_RS22740 0.10 +1.3
Or see this region's nucleotide sequence