Experiment: R2A with Lomefloxacin 0.00075 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20715 and RR42_RS20720 are separated by 55 nucleotides RR42_RS20720 and RR42_RS20725 overlap by 4 nucleotides RR42_RS20725 and RR42_RS20730 are separated by 61 nucleotides RR42_RS20730 and RR42_RS20735 overlap by 4 nucleotides
RR42_RS20715: RR42_RS20715 - 4-hydroxyacetophenone monooxygenase, at 4,498,637 to 4,500,211
_RS20715
RR42_RS20720: RR42_RS20720 - short-chain dehydrogenase, at 4,500,267 to 4,501,181
_RS20720
RR42_RS20725: RR42_RS20725 - alpha/beta hydrolase, at 4,501,178 to 4,502,140
_RS20725
RR42_RS20730: RR42_RS20730 - metal-dependent hydrolase, at 4,502,202 to 4,503,074
_RS20730
RR42_RS20735: RR42_RS20735 - rubredoxin, at 4,503,071 to 4,503,235
_RS20735
Position (kb)
4501
4502
4503 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4500.463 kb on + strand, within RR42_RS20720 at 4500.464 kb on - strand, within RR42_RS20720 at 4500.483 kb on + strand, within RR42_RS20720 at 4500.483 kb on + strand, within RR42_RS20720 at 4500.483 kb on + strand, within RR42_RS20720 at 4500.484 kb on - strand, within RR42_RS20720 at 4500.484 kb on - strand, within RR42_RS20720 at 4500.484 kb on - strand, within RR42_RS20720 at 4500.582 kb on + strand, within RR42_RS20720 at 4500.582 kb on + strand, within RR42_RS20720 at 4500.582 kb on + strand, within RR42_RS20720 at 4500.583 kb on - strand, within RR42_RS20720 at 4500.583 kb on - strand, within RR42_RS20720 at 4500.583 kb on - strand, within RR42_RS20720 at 4500.583 kb on - strand, within RR42_RS20720 at 4500.690 kb on - strand, within RR42_RS20720 at 4500.690 kb on - strand, within RR42_RS20720 at 4500.750 kb on + strand, within RR42_RS20720 at 4500.936 kb on + strand, within RR42_RS20720 at 4500.936 kb on + strand, within RR42_RS20720 at 4500.937 kb on - strand, within RR42_RS20720 at 4501.302 kb on - strand, within RR42_RS20725 at 4501.302 kb on - strand, within RR42_RS20725 at 4501.352 kb on + strand, within RR42_RS20725 at 4501.353 kb on - strand, within RR42_RS20725 at 4501.353 kb on - strand, within RR42_RS20725 at 4501.353 kb on - strand, within RR42_RS20725 at 4501.546 kb on + strand, within RR42_RS20725 at 4501.547 kb on - strand, within RR42_RS20725 at 4501.550 kb on + strand, within RR42_RS20725 at 4501.550 kb on + strand, within RR42_RS20725 at 4501.550 kb on + strand, within RR42_RS20725 at 4501.587 kb on - strand, within RR42_RS20725 at 4501.721 kb on + strand, within RR42_RS20725 at 4501.721 kb on + strand, within RR42_RS20725 at 4501.721 kb on + strand, within RR42_RS20725 at 4501.724 kb on - strand, within RR42_RS20725 at 4501.811 kb on + strand, within RR42_RS20725 at 4502.058 kb on - strand at 4502.194 kb on + strand at 4502.194 kb on + strand at 4502.194 kb on + strand at 4502.195 kb on - strand at 4502.405 kb on + strand, within RR42_RS20730 at 4502.438 kb on + strand, within RR42_RS20730 at 4502.438 kb on + strand, within RR42_RS20730 at 4502.439 kb on - strand, within RR42_RS20730 at 4502.446 kb on - strand, within RR42_RS20730 at 4502.446 kb on - strand, within RR42_RS20730 at 4502.446 kb on - strand, within RR42_RS20730 at 4502.531 kb on - strand, within RR42_RS20730 at 4502.700 kb on + strand, within RR42_RS20730 at 4502.701 kb on - strand, within RR42_RS20730 at 4502.736 kb on + strand, within RR42_RS20730 at 4502.737 kb on - strand, within RR42_RS20730 at 4502.743 kb on - strand, within RR42_RS20730 at 4502.931 kb on + strand, within RR42_RS20730 at 4503.107 kb on + strand, within RR42_RS20735
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM remove 4,500,463 + RR42_RS20720 0.21 -1.1 4,500,464 - RR42_RS20720 0.22 +0.2 4,500,483 + RR42_RS20720 0.24 +0.6 4,500,483 + RR42_RS20720 0.24 +1.0 4,500,483 + RR42_RS20720 0.24 +1.7 4,500,484 - RR42_RS20720 0.24 +1.1 4,500,484 - RR42_RS20720 0.24 -0.1 4,500,484 - RR42_RS20720 0.24 -0.1 4,500,582 + RR42_RS20720 0.34 -0.2 4,500,582 + RR42_RS20720 0.34 -0.9 4,500,582 + RR42_RS20720 0.34 -1.1 4,500,583 - RR42_RS20720 0.35 -1.2 4,500,583 - RR42_RS20720 0.35 +1.1 4,500,583 - RR42_RS20720 0.35 -0.3 4,500,583 - RR42_RS20720 0.35 +2.0 4,500,690 - RR42_RS20720 0.46 +0.6 4,500,690 - RR42_RS20720 0.46 +0.3 4,500,750 + RR42_RS20720 0.53 +0.7 4,500,936 + RR42_RS20720 0.73 +1.4 4,500,936 + RR42_RS20720 0.73 -0.7 4,500,937 - RR42_RS20720 0.73 +0.3 4,501,302 - RR42_RS20725 0.13 +0.4 4,501,302 - RR42_RS20725 0.13 -0.9 4,501,352 + RR42_RS20725 0.18 -0.2 4,501,353 - RR42_RS20725 0.18 -0.1 4,501,353 - RR42_RS20725 0.18 -1.1 4,501,353 - RR42_RS20725 0.18 +1.5 4,501,546 + RR42_RS20725 0.38 +0.2 4,501,547 - RR42_RS20725 0.38 +0.9 4,501,550 + RR42_RS20725 0.39 +1.3 4,501,550 + RR42_RS20725 0.39 +2.8 4,501,550 + RR42_RS20725 0.39 +0.4 4,501,587 - RR42_RS20725 0.42 -0.2 4,501,721 + RR42_RS20725 0.56 +0.7 4,501,721 + RR42_RS20725 0.56 +0.1 4,501,721 + RR42_RS20725 0.56 -0.1 4,501,724 - RR42_RS20725 0.57 -0.7 4,501,811 + RR42_RS20725 0.66 +2.8 4,502,058 - +0.2 4,502,194 + +0.6 4,502,194 + -0.3 4,502,194 + -0.8 4,502,195 - +0.0 4,502,405 + RR42_RS20730 0.23 -2.8 4,502,438 + RR42_RS20730 0.27 -0.8 4,502,438 + RR42_RS20730 0.27 +0.2 4,502,439 - RR42_RS20730 0.27 +0.8 4,502,446 - RR42_RS20730 0.28 +1.3 4,502,446 - RR42_RS20730 0.28 -0.4 4,502,446 - RR42_RS20730 0.28 -0.3 4,502,531 - RR42_RS20730 0.38 -0.2 4,502,700 + RR42_RS20730 0.57 -0.2 4,502,701 - RR42_RS20730 0.57 -0.4 4,502,736 + RR42_RS20730 0.61 +0.0 4,502,737 - RR42_RS20730 0.61 +0.2 4,502,743 - RR42_RS20730 0.62 +0.3 4,502,931 + RR42_RS20730 0.84 +0.5 4,503,107 + RR42_RS20735 0.22 -0.1
Or see this region's nucleotide sequence