Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS20645

Experiment: R2A with Lomefloxacin 0.00075 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS20640 and RR42_RS20645 are separated by 447 nucleotidesRR42_RS20645 and RR42_RS20650 are separated by 30 nucleotides RR42_RS20640: RR42_RS20640 - TetR family transcriptional regulator, at 4,480,010 to 4,480,765 _RS20640 RR42_RS20645: RR42_RS20645 - MexE family multidrug efflux RND transporter periplasmic adaptor subunit, at 4,481,213 to 4,482,445 _RS20645 RR42_RS20650: RR42_RS20650 - multidrug efflux RND transporter permease subunit, at 4,482,476 to 4,485,628 _RS20650 Position (kb) 4481 4482 4483Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4 5 6 7 8at 4480.879 kb on - strandat 4480.879 kb on - strandat 4480.879 kb on - strandat 4480.879 kb on - strandat 4480.879 kb on - strandat 4480.879 kb on - strandat 4480.881 kb on - strandat 4480.883 kb on - strandat 4480.883 kb on - strandat 4480.937 kb on + strandat 4480.938 kb on - strandat 4480.940 kb on - strandat 4481.045 kb on + strandat 4481.046 kb on - strandat 4481.096 kb on + strandat 4481.097 kb on - strandat 4481.103 kb on - strandat 4481.103 kb on - strandat 4481.209 kb on - strandat 4481.230 kb on + strandat 4482.444 kb on - strandat 4482.475 kb on + strandat 4482.475 kb on + strandat 4482.476 kb on - strandat 4482.476 kb on - strandat 4482.491 kb on - strandat 4482.689 kb on - strandat 4482.704 kb on + strandat 4482.704 kb on + strandat 4482.705 kb on - strandat 4482.705 kb on - strandat 4482.705 kb on - strandat 4482.707 kb on + strandat 4482.707 kb on + strandat 4482.707 kb on + strandat 4482.881 kb on + strand, within RR42_RS20650at 4482.881 kb on + strand, within RR42_RS20650at 4482.881 kb on + strand, within RR42_RS20650at 4482.882 kb on - strand, within RR42_RS20650at 4482.882 kb on - strand, within RR42_RS20650at 4482.882 kb on - strand, within RR42_RS20650at 4482.882 kb on - strand, within RR42_RS20650at 4482.882 kb on - strand, within RR42_RS20650at 4482.882 kb on - strand, within RR42_RS20650at 4482.944 kb on + strand, within RR42_RS20650at 4482.944 kb on + strand, within RR42_RS20650at 4482.944 kb on + strand, within RR42_RS20650at 4482.944 kb on + strand, within RR42_RS20650at 4482.944 kb on + strand, within RR42_RS20650at 4482.945 kb on - strand, within RR42_RS20650at 4483.019 kb on + strand, within RR42_RS20650at 4483.020 kb on - strand, within RR42_RS20650at 4483.020 kb on - strand, within RR42_RS20650at 4483.178 kb on - strand, within RR42_RS20650at 4483.299 kb on - strand, within RR42_RS20650at 4483.442 kb on + strand, within RR42_RS20650at 4483.442 kb on + strand, within RR42_RS20650at 4483.443 kb on - strand, within RR42_RS20650at 4483.443 kb on - strand, within RR42_RS20650at 4483.443 kb on - strand, within RR42_RS20650

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM
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4,480,879 - -1.8
4,480,879 - -2.2
4,480,879 - -0.6
4,480,879 - -1.9
4,480,879 - -1.3
4,480,879 - -1.4
4,480,881 - -0.8
4,480,883 - -1.7
4,480,883 - -0.8
4,480,937 + +8.0
4,480,938 - +4.0
4,480,940 - -1.9
4,481,045 + +1.0
4,481,046 - -2.9
4,481,096 + +6.4
4,481,097 - -1.8
4,481,103 - -1.8
4,481,103 - -1.2
4,481,209 - -4.0
4,481,230 + -1.8
4,482,444 - -1.8
4,482,475 + -0.7
4,482,475 + -0.5
4,482,476 - -3.1
4,482,476 - +0.5
4,482,491 - -3.0
4,482,689 - -3.3
4,482,704 + -2.1
4,482,704 + -2.4
4,482,705 - -1.6
4,482,705 - -2.3
4,482,705 - -2.6
4,482,707 + -0.1
4,482,707 + -2.4
4,482,707 + -1.1
4,482,881 + RR42_RS20650 0.13 -2.1
4,482,881 + RR42_RS20650 0.13 -1.7
4,482,881 + RR42_RS20650 0.13 -0.2
4,482,882 - RR42_RS20650 0.13 -3.0
4,482,882 - RR42_RS20650 0.13 -0.5
4,482,882 - RR42_RS20650 0.13 -2.5
4,482,882 - RR42_RS20650 0.13 +0.7
4,482,882 - RR42_RS20650 0.13 -1.9
4,482,882 - RR42_RS20650 0.13 -2.1
4,482,944 + RR42_RS20650 0.15 -1.2
4,482,944 + RR42_RS20650 0.15 -2.1
4,482,944 + RR42_RS20650 0.15 +0.5
4,482,944 + RR42_RS20650 0.15 -1.6
4,482,944 + RR42_RS20650 0.15 -0.1
4,482,945 - RR42_RS20650 0.15 +0.5
4,483,019 + RR42_RS20650 0.17 -2.0
4,483,020 - RR42_RS20650 0.17 -1.1
4,483,020 - RR42_RS20650 0.17 -1.9
4,483,178 - RR42_RS20650 0.22 -2.0
4,483,299 - RR42_RS20650 0.26 -1.9
4,483,442 + RR42_RS20650 0.31 -1.6
4,483,442 + RR42_RS20650 0.31 -1.2
4,483,443 - RR42_RS20650 0.31 -1.1
4,483,443 - RR42_RS20650 0.31 -2.4
4,483,443 - RR42_RS20650 0.31 -3.3

Or see this region's nucleotide sequence