Experiment: R2A with Lomefloxacin 0.00075 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20250 and RR42_RS20255 are separated by 407 nucleotides RR42_RS20255 and RR42_RS20260 are separated by 81 nucleotides RR42_RS20260 and RR42_RS20265 are separated by 277 nucleotides
RR42_RS20250: RR42_RS20250 - branched-chain amino acid ABC transporter substrate-binding protein, at 4,380,568 to 4,381,719
_RS20250
RR42_RS20255: RR42_RS20255 - membrane protein, at 4,382,127 to 4,382,885
_RS20255
RR42_RS20260: RR42_RS20260 - hypothetical protein, at 4,382,967 to 4,383,518
_RS20260
RR42_RS20265: RR42_RS20265 - ABC transporter permease, at 4,383,796 to 4,384,824
_RS20265
Position (kb)
4382
4383 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4381.217 kb on + strand, within RR42_RS20250 at 4381.217 kb on + strand, within RR42_RS20250 at 4381.217 kb on + strand, within RR42_RS20250 at 4381.218 kb on - strand, within RR42_RS20250 at 4381.218 kb on - strand, within RR42_RS20250 at 4381.236 kb on + strand, within RR42_RS20250 at 4381.450 kb on + strand, within RR42_RS20250 at 4381.450 kb on + strand, within RR42_RS20250 at 4381.450 kb on + strand, within RR42_RS20250 at 4381.451 kb on - strand, within RR42_RS20250 at 4381.451 kb on - strand, within RR42_RS20250 at 4381.451 kb on - strand, within RR42_RS20250 at 4381.451 kb on - strand, within RR42_RS20250 at 4381.535 kb on + strand, within RR42_RS20250 at 4381.536 kb on - strand, within RR42_RS20250 at 4381.633 kb on + strand at 4381.634 kb on - strand at 4381.824 kb on + strand at 4381.824 kb on + strand at 4381.824 kb on + strand at 4381.825 kb on - strand at 4381.825 kb on - strand at 4381.916 kb on + strand at 4382.051 kb on - strand at 4382.051 kb on - strand at 4382.062 kb on + strand at 4382.256 kb on - strand, within RR42_RS20255 at 4382.277 kb on + strand, within RR42_RS20255 at 4382.277 kb on + strand, within RR42_RS20255 at 4382.278 kb on - strand, within RR42_RS20255 at 4382.446 kb on + strand, within RR42_RS20255 at 4382.446 kb on + strand, within RR42_RS20255 at 4382.446 kb on + strand, within RR42_RS20255 at 4382.546 kb on + strand, within RR42_RS20255 at 4382.546 kb on + strand, within RR42_RS20255 at 4382.546 kb on + strand, within RR42_RS20255 at 4382.547 kb on - strand, within RR42_RS20255 at 4382.547 kb on - strand, within RR42_RS20255 at 4382.547 kb on - strand, within RR42_RS20255 at 4382.549 kb on - strand, within RR42_RS20255 at 4382.549 kb on - strand, within RR42_RS20255 at 4382.571 kb on - strand, within RR42_RS20255 at 4382.571 kb on - strand, within RR42_RS20255 at 4382.621 kb on - strand, within RR42_RS20255 at 4382.680 kb on + strand, within RR42_RS20255 at 4382.680 kb on + strand, within RR42_RS20255 at 4382.681 kb on - strand, within RR42_RS20255 at 4382.681 kb on - strand, within RR42_RS20255 at 4382.681 kb on - strand, within RR42_RS20255 at 4382.681 kb on - strand, within RR42_RS20255 at 4382.701 kb on + strand, within RR42_RS20255 at 4382.724 kb on - strand, within RR42_RS20255 at 4382.724 kb on - strand, within RR42_RS20255 at 4382.724 kb on - strand, within RR42_RS20255 at 4382.724 kb on - strand, within RR42_RS20255 at 4382.724 kb on - strand, within RR42_RS20255 at 4382.976 kb on + strand at 4382.976 kb on + strand at 4382.977 kb on - strand at 4383.226 kb on - strand, within RR42_RS20260 at 4383.420 kb on + strand, within RR42_RS20260 at 4383.420 kb on + strand, within RR42_RS20260 at 4383.421 kb on - strand, within RR42_RS20260 at 4383.582 kb on + strand at 4383.583 kb on - strand at 4383.583 kb on - strand at 4383.634 kb on + strand at 4383.635 kb on - strand at 4383.635 kb on - strand at 4383.635 kb on - strand at 4383.635 kb on - strand at 4383.639 kb on + strand at 4383.639 kb on + strand at 4383.640 kb on - strand at 4383.641 kb on + strand at 4383.642 kb on - strand at 4383.642 kb on - strand at 4383.657 kb on + strand at 4383.657 kb on + strand at 4383.699 kb on + strand at 4383.700 kb on - strand at 4383.715 kb on + strand at 4383.715 kb on + strand at 4383.716 kb on - strand at 4383.718 kb on - strand at 4383.726 kb on + strand at 4383.726 kb on + strand at 4383.727 kb on - strand at 4383.727 kb on - strand at 4383.753 kb on + strand at 4383.859 kb on + strand at 4383.860 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM remove 4,381,217 + RR42_RS20250 0.56 -0.6 4,381,217 + RR42_RS20250 0.56 +0.2 4,381,217 + RR42_RS20250 0.56 -0.2 4,381,218 - RR42_RS20250 0.56 -0.3 4,381,218 - RR42_RS20250 0.56 +3.3 4,381,236 + RR42_RS20250 0.58 -1.2 4,381,450 + RR42_RS20250 0.77 +0.2 4,381,450 + RR42_RS20250 0.77 -0.2 4,381,450 + RR42_RS20250 0.77 +0.4 4,381,451 - RR42_RS20250 0.77 +1.1 4,381,451 - RR42_RS20250 0.77 +0.5 4,381,451 - RR42_RS20250 0.77 +0.7 4,381,451 - RR42_RS20250 0.77 +0.6 4,381,535 + RR42_RS20250 0.84 +1.3 4,381,536 - RR42_RS20250 0.84 -1.3 4,381,633 + -0.3 4,381,634 - +3.1 4,381,824 + +0.0 4,381,824 + +0.1 4,381,824 + +0.5 4,381,825 - +0.7 4,381,825 - -0.1 4,381,916 + +2.1 4,382,051 - +0.9 4,382,051 - +1.1 4,382,062 + -0.3 4,382,256 - RR42_RS20255 0.17 -0.7 4,382,277 + RR42_RS20255 0.20 +0.2 4,382,277 + RR42_RS20255 0.20 -0.3 4,382,278 - RR42_RS20255 0.20 -0.4 4,382,446 + RR42_RS20255 0.42 +2.5 4,382,446 + RR42_RS20255 0.42 -0.0 4,382,446 + RR42_RS20255 0.42 +0.9 4,382,546 + RR42_RS20255 0.55 +0.4 4,382,546 + RR42_RS20255 0.55 +1.7 4,382,546 + RR42_RS20255 0.55 +0.3 4,382,547 - RR42_RS20255 0.55 +0.8 4,382,547 - RR42_RS20255 0.55 +1.1 4,382,547 - RR42_RS20255 0.55 -0.2 4,382,549 - RR42_RS20255 0.56 +1.4 4,382,549 - RR42_RS20255 0.56 -0.1 4,382,571 - RR42_RS20255 0.58 +0.3 4,382,571 - RR42_RS20255 0.58 -1.0 4,382,621 - RR42_RS20255 0.65 -0.1 4,382,680 + RR42_RS20255 0.73 +0.2 4,382,680 + RR42_RS20255 0.73 +1.6 4,382,681 - RR42_RS20255 0.73 +0.1 4,382,681 - RR42_RS20255 0.73 -2.0 4,382,681 - RR42_RS20255 0.73 -1.3 4,382,681 - RR42_RS20255 0.73 +0.4 4,382,701 + RR42_RS20255 0.76 +0.0 4,382,724 - RR42_RS20255 0.79 +0.4 4,382,724 - RR42_RS20255 0.79 +0.2 4,382,724 - RR42_RS20255 0.79 +2.1 4,382,724 - RR42_RS20255 0.79 -0.9 4,382,724 - RR42_RS20255 0.79 +0.2 4,382,976 + -0.9 4,382,976 + -0.5 4,382,977 - +1.5 4,383,226 - RR42_RS20260 0.47 -2.2 4,383,420 + RR42_RS20260 0.82 -0.5 4,383,420 + RR42_RS20260 0.82 +0.0 4,383,421 - RR42_RS20260 0.82 -1.1 4,383,582 + +0.7 4,383,583 - +0.2 4,383,583 - +0.8 4,383,634 + +0.8 4,383,635 - +2.1 4,383,635 - +0.6 4,383,635 - +1.8 4,383,635 - +0.3 4,383,639 + +1.8 4,383,639 + +2.0 4,383,640 - +1.3 4,383,641 + +0.3 4,383,642 - +1.6 4,383,642 - +0.8 4,383,657 + +2.2 4,383,657 + +1.7 4,383,699 + +2.3 4,383,700 - +0.9 4,383,715 + +1.4 4,383,715 + +2.3 4,383,716 - +1.2 4,383,718 - +1.7 4,383,726 + +2.2 4,383,726 + +1.3 4,383,727 - +1.2 4,383,727 - +0.9 4,383,753 + +2.6 4,383,859 + +0.4 4,383,860 - +0.3
Or see this region's nucleotide sequence