Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS20255

Experiment: R2A with Lomefloxacin 0.00075 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS20250 and RR42_RS20255 are separated by 407 nucleotidesRR42_RS20255 and RR42_RS20260 are separated by 81 nucleotidesRR42_RS20260 and RR42_RS20265 are separated by 277 nucleotides RR42_RS20250: RR42_RS20250 - branched-chain amino acid ABC transporter substrate-binding protein, at 4,380,568 to 4,381,719 _RS20250 RR42_RS20255: RR42_RS20255 - membrane protein, at 4,382,127 to 4,382,885 _RS20255 RR42_RS20260: RR42_RS20260 - hypothetical protein, at 4,382,967 to 4,383,518 _RS20260 RR42_RS20265: RR42_RS20265 - ABC transporter permease, at 4,383,796 to 4,384,824 _RS20265 Position (kb) 4382 4383Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4381.217 kb on + strand, within RR42_RS20250at 4381.217 kb on + strand, within RR42_RS20250at 4381.217 kb on + strand, within RR42_RS20250at 4381.218 kb on - strand, within RR42_RS20250at 4381.218 kb on - strand, within RR42_RS20250at 4381.236 kb on + strand, within RR42_RS20250at 4381.450 kb on + strand, within RR42_RS20250at 4381.450 kb on + strand, within RR42_RS20250at 4381.450 kb on + strand, within RR42_RS20250at 4381.451 kb on - strand, within RR42_RS20250at 4381.451 kb on - strand, within RR42_RS20250at 4381.451 kb on - strand, within RR42_RS20250at 4381.451 kb on - strand, within RR42_RS20250at 4381.535 kb on + strand, within RR42_RS20250at 4381.536 kb on - strand, within RR42_RS20250at 4381.633 kb on + strandat 4381.634 kb on - strandat 4381.824 kb on + strandat 4381.824 kb on + strandat 4381.824 kb on + strandat 4381.825 kb on - strandat 4381.825 kb on - strandat 4381.916 kb on + strandat 4382.051 kb on - strandat 4382.051 kb on - strandat 4382.062 kb on + strandat 4382.256 kb on - strand, within RR42_RS20255at 4382.277 kb on + strand, within RR42_RS20255at 4382.277 kb on + strand, within RR42_RS20255at 4382.278 kb on - strand, within RR42_RS20255at 4382.446 kb on + strand, within RR42_RS20255at 4382.446 kb on + strand, within RR42_RS20255at 4382.446 kb on + strand, within RR42_RS20255at 4382.546 kb on + strand, within RR42_RS20255at 4382.546 kb on + strand, within RR42_RS20255at 4382.546 kb on + strand, within RR42_RS20255at 4382.547 kb on - strand, within RR42_RS20255at 4382.547 kb on - strand, within RR42_RS20255at 4382.547 kb on - strand, within RR42_RS20255at 4382.549 kb on - strand, within RR42_RS20255at 4382.549 kb on - strand, within RR42_RS20255at 4382.571 kb on - strand, within RR42_RS20255at 4382.571 kb on - strand, within RR42_RS20255at 4382.621 kb on - strand, within RR42_RS20255at 4382.680 kb on + strand, within RR42_RS20255at 4382.680 kb on + strand, within RR42_RS20255at 4382.681 kb on - strand, within RR42_RS20255at 4382.681 kb on - strand, within RR42_RS20255at 4382.681 kb on - strand, within RR42_RS20255at 4382.681 kb on - strand, within RR42_RS20255at 4382.701 kb on + strand, within RR42_RS20255at 4382.724 kb on - strand, within RR42_RS20255at 4382.724 kb on - strand, within RR42_RS20255at 4382.724 kb on - strand, within RR42_RS20255at 4382.724 kb on - strand, within RR42_RS20255at 4382.724 kb on - strand, within RR42_RS20255at 4382.976 kb on + strandat 4382.976 kb on + strandat 4382.977 kb on - strandat 4383.226 kb on - strand, within RR42_RS20260at 4383.420 kb on + strand, within RR42_RS20260at 4383.420 kb on + strand, within RR42_RS20260at 4383.421 kb on - strand, within RR42_RS20260at 4383.582 kb on + strandat 4383.583 kb on - strandat 4383.583 kb on - strandat 4383.634 kb on + strandat 4383.635 kb on - strandat 4383.635 kb on - strandat 4383.635 kb on - strandat 4383.635 kb on - strandat 4383.639 kb on + strandat 4383.639 kb on + strandat 4383.640 kb on - strandat 4383.641 kb on + strandat 4383.642 kb on - strandat 4383.642 kb on - strandat 4383.657 kb on + strandat 4383.657 kb on + strandat 4383.699 kb on + strandat 4383.700 kb on - strandat 4383.715 kb on + strandat 4383.715 kb on + strandat 4383.716 kb on - strandat 4383.718 kb on - strandat 4383.726 kb on + strandat 4383.726 kb on + strandat 4383.727 kb on - strandat 4383.727 kb on - strandat 4383.753 kb on + strandat 4383.859 kb on + strandat 4383.860 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM
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4,381,217 + RR42_RS20250 0.56 -0.6
4,381,217 + RR42_RS20250 0.56 +0.2
4,381,217 + RR42_RS20250 0.56 -0.2
4,381,218 - RR42_RS20250 0.56 -0.3
4,381,218 - RR42_RS20250 0.56 +3.3
4,381,236 + RR42_RS20250 0.58 -1.2
4,381,450 + RR42_RS20250 0.77 +0.2
4,381,450 + RR42_RS20250 0.77 -0.2
4,381,450 + RR42_RS20250 0.77 +0.4
4,381,451 - RR42_RS20250 0.77 +1.1
4,381,451 - RR42_RS20250 0.77 +0.5
4,381,451 - RR42_RS20250 0.77 +0.7
4,381,451 - RR42_RS20250 0.77 +0.6
4,381,535 + RR42_RS20250 0.84 +1.3
4,381,536 - RR42_RS20250 0.84 -1.3
4,381,633 + -0.3
4,381,634 - +3.1
4,381,824 + +0.0
4,381,824 + +0.1
4,381,824 + +0.5
4,381,825 - +0.7
4,381,825 - -0.1
4,381,916 + +2.1
4,382,051 - +0.9
4,382,051 - +1.1
4,382,062 + -0.3
4,382,256 - RR42_RS20255 0.17 -0.7
4,382,277 + RR42_RS20255 0.20 +0.2
4,382,277 + RR42_RS20255 0.20 -0.3
4,382,278 - RR42_RS20255 0.20 -0.4
4,382,446 + RR42_RS20255 0.42 +2.5
4,382,446 + RR42_RS20255 0.42 -0.0
4,382,446 + RR42_RS20255 0.42 +0.9
4,382,546 + RR42_RS20255 0.55 +0.4
4,382,546 + RR42_RS20255 0.55 +1.7
4,382,546 + RR42_RS20255 0.55 +0.3
4,382,547 - RR42_RS20255 0.55 +0.8
4,382,547 - RR42_RS20255 0.55 +1.1
4,382,547 - RR42_RS20255 0.55 -0.2
4,382,549 - RR42_RS20255 0.56 +1.4
4,382,549 - RR42_RS20255 0.56 -0.1
4,382,571 - RR42_RS20255 0.58 +0.3
4,382,571 - RR42_RS20255 0.58 -1.0
4,382,621 - RR42_RS20255 0.65 -0.1
4,382,680 + RR42_RS20255 0.73 +0.2
4,382,680 + RR42_RS20255 0.73 +1.6
4,382,681 - RR42_RS20255 0.73 +0.1
4,382,681 - RR42_RS20255 0.73 -2.0
4,382,681 - RR42_RS20255 0.73 -1.3
4,382,681 - RR42_RS20255 0.73 +0.4
4,382,701 + RR42_RS20255 0.76 +0.0
4,382,724 - RR42_RS20255 0.79 +0.4
4,382,724 - RR42_RS20255 0.79 +0.2
4,382,724 - RR42_RS20255 0.79 +2.1
4,382,724 - RR42_RS20255 0.79 -0.9
4,382,724 - RR42_RS20255 0.79 +0.2
4,382,976 + -0.9
4,382,976 + -0.5
4,382,977 - +1.5
4,383,226 - RR42_RS20260 0.47 -2.2
4,383,420 + RR42_RS20260 0.82 -0.5
4,383,420 + RR42_RS20260 0.82 +0.0
4,383,421 - RR42_RS20260 0.82 -1.1
4,383,582 + +0.7
4,383,583 - +0.2
4,383,583 - +0.8
4,383,634 + +0.8
4,383,635 - +2.1
4,383,635 - +0.6
4,383,635 - +1.8
4,383,635 - +0.3
4,383,639 + +1.8
4,383,639 + +2.0
4,383,640 - +1.3
4,383,641 + +0.3
4,383,642 - +1.6
4,383,642 - +0.8
4,383,657 + +2.2
4,383,657 + +1.7
4,383,699 + +2.3
4,383,700 - +0.9
4,383,715 + +1.4
4,383,715 + +2.3
4,383,716 - +1.2
4,383,718 - +1.7
4,383,726 + +2.2
4,383,726 + +1.3
4,383,727 - +1.2
4,383,727 - +0.9
4,383,753 + +2.6
4,383,859 + +0.4
4,383,860 - +0.3

Or see this region's nucleotide sequence