Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS19865

Experiment: R2A with Lomefloxacin 0.00075 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS19860 and RR42_RS19865 are separated by 88 nucleotidesRR42_RS19865 and RR42_RS19870 are separated by 64 nucleotides RR42_RS19860: RR42_RS19860 - tartronate semialdehyde reductase, at 4,287,609 to 4,288,535 _RS19860 RR42_RS19865: RR42_RS19865 - hydroxypyruvate reductase, at 4,288,624 to 4,289,937 _RS19865 RR42_RS19870: RR42_RS19870 - pyruvate kinase, at 4,290,002 to 4,291,480 _RS19870 Position (kb) 4288 4289 4290Strain fitness (log2 ratio) -1 0 1 2 3 4at 4287.636 kb on - strandat 4287.981 kb on + strand, within RR42_RS19860at 4288.313 kb on + strand, within RR42_RS19860at 4288.533 kb on + strandat 4288.574 kb on - strandat 4288.793 kb on + strand, within RR42_RS19865at 4288.793 kb on + strand, within RR42_RS19865at 4289.108 kb on + strand, within RR42_RS19865at 4289.109 kb on - strand, within RR42_RS19865at 4289.109 kb on - strand, within RR42_RS19865at 4289.320 kb on + strand, within RR42_RS19865at 4289.320 kb on + strand, within RR42_RS19865at 4289.321 kb on - strand, within RR42_RS19865at 4289.935 kb on + strandat 4290.899 kb on + strand, within RR42_RS19870at 4290.900 kb on - strand, within RR42_RS19870at 4290.900 kb on - strand, within RR42_RS19870at 4290.900 kb on - strand, within RR42_RS19870

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM
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4,287,636 - -1.0
4,287,981 + RR42_RS19860 0.40 -0.7
4,288,313 + RR42_RS19860 0.76 -1.3
4,288,533 + -0.6
4,288,574 - +0.9
4,288,793 + RR42_RS19865 0.13 -0.4
4,288,793 + RR42_RS19865 0.13 -0.1
4,289,108 + RR42_RS19865 0.37 +0.8
4,289,109 - RR42_RS19865 0.37 +2.0
4,289,109 - RR42_RS19865 0.37 +1.6
4,289,320 + RR42_RS19865 0.53 -0.2
4,289,320 + RR42_RS19865 0.53 +1.0
4,289,321 - RR42_RS19865 0.53 -0.4
4,289,935 + +0.0
4,290,899 + RR42_RS19870 0.61 +0.8
4,290,900 - RR42_RS19870 0.61 +4.3
4,290,900 - RR42_RS19870 0.61 -1.7
4,290,900 - RR42_RS19870 0.61 +1.1

Or see this region's nucleotide sequence