Experiment: R2A with Lomefloxacin 0.00075 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS17455 and RR42_RS17460 are separated by 450 nucleotides RR42_RS17460 and RR42_RS17465 are separated by 86 nucleotides
RR42_RS17455: RR42_RS17455 - adenylosuccinate lyase, at 3,778,631 to 3,780,007
_RS17455
RR42_RS17460: RR42_RS17460 - glutathione S-transferase, at 3,780,458 to 3,781,072
_RS17460
RR42_RS17465: RR42_RS17465 - tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase, at 3,781,159 to 3,782,259
_RS17465
Position (kb)
3780
3781
3782 Strain fitness (log2 ratio)
-2
-1
0
1 at 3780.356 kb on + strand at 3780.357 kb on - strand at 3780.377 kb on + strand at 3780.437 kb on + strand at 3780.437 kb on + strand at 3780.438 kb on - strand at 3780.438 kb on - strand at 3780.438 kb on - strand at 3780.467 kb on + strand at 3780.467 kb on + strand at 3780.467 kb on + strand at 3780.467 kb on + strand at 3780.467 kb on + strand at 3780.467 kb on + strand at 3780.467 kb on + strand at 3780.468 kb on - strand at 3780.468 kb on - strand at 3780.468 kb on - strand at 3780.468 kb on - strand at 3780.603 kb on + strand, within RR42_RS17460 at 3780.603 kb on + strand, within RR42_RS17460 at 3780.604 kb on - strand, within RR42_RS17460 at 3780.727 kb on - strand, within RR42_RS17460 at 3780.914 kb on + strand, within RR42_RS17460 at 3780.915 kb on - strand, within RR42_RS17460 at 3780.953 kb on + strand, within RR42_RS17460 at 3781.013 kb on + strand at 3781.013 kb on + strand at 3781.014 kb on - strand at 3781.014 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM remove 3,780,356 + -0.0 3,780,357 - -0.6 3,780,377 + +0.3 3,780,437 + -0.1 3,780,437 + +0.7 3,780,438 - -0.9 3,780,438 - -0.4 3,780,438 - +0.2 3,780,467 + +1.4 3,780,467 + -0.7 3,780,467 + -0.8 3,780,467 + -0.2 3,780,467 + -0.5 3,780,467 + +1.1 3,780,467 + +0.5 3,780,468 - +0.1 3,780,468 - -2.0 3,780,468 - -0.9 3,780,468 - +0.3 3,780,603 + RR42_RS17460 0.24 -0.3 3,780,603 + RR42_RS17460 0.24 -0.5 3,780,604 - RR42_RS17460 0.24 +0.4 3,780,727 - RR42_RS17460 0.44 -2.7 3,780,914 + RR42_RS17460 0.74 +0.5 3,780,915 - RR42_RS17460 0.74 -1.1 3,780,953 + RR42_RS17460 0.80 +0.3 3,781,013 + -1.6 3,781,013 + +1.0 3,781,014 - +0.4 3,781,014 - +0.8
Or see this region's nucleotide sequence