Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS17460

Experiment: R2A with Lomefloxacin 0.00075 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS17455 and RR42_RS17460 are separated by 450 nucleotidesRR42_RS17460 and RR42_RS17465 are separated by 86 nucleotides RR42_RS17455: RR42_RS17455 - adenylosuccinate lyase, at 3,778,631 to 3,780,007 _RS17455 RR42_RS17460: RR42_RS17460 - glutathione S-transferase, at 3,780,458 to 3,781,072 _RS17460 RR42_RS17465: RR42_RS17465 - tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase, at 3,781,159 to 3,782,259 _RS17465 Position (kb) 3780 3781 3782Strain fitness (log2 ratio) -2 -1 0 1at 3780.356 kb on + strandat 3780.357 kb on - strandat 3780.377 kb on + strandat 3780.437 kb on + strandat 3780.437 kb on + strandat 3780.438 kb on - strandat 3780.438 kb on - strandat 3780.438 kb on - strandat 3780.467 kb on + strandat 3780.467 kb on + strandat 3780.467 kb on + strandat 3780.467 kb on + strandat 3780.467 kb on + strandat 3780.467 kb on + strandat 3780.467 kb on + strandat 3780.468 kb on - strandat 3780.468 kb on - strandat 3780.468 kb on - strandat 3780.468 kb on - strandat 3780.603 kb on + strand, within RR42_RS17460at 3780.603 kb on + strand, within RR42_RS17460at 3780.604 kb on - strand, within RR42_RS17460at 3780.727 kb on - strand, within RR42_RS17460at 3780.914 kb on + strand, within RR42_RS17460at 3780.915 kb on - strand, within RR42_RS17460at 3780.953 kb on + strand, within RR42_RS17460at 3781.013 kb on + strandat 3781.013 kb on + strandat 3781.014 kb on - strandat 3781.014 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM
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3,780,356 + -0.0
3,780,357 - -0.6
3,780,377 + +0.3
3,780,437 + -0.1
3,780,437 + +0.7
3,780,438 - -0.9
3,780,438 - -0.4
3,780,438 - +0.2
3,780,467 + +1.4
3,780,467 + -0.7
3,780,467 + -0.8
3,780,467 + -0.2
3,780,467 + -0.5
3,780,467 + +1.1
3,780,467 + +0.5
3,780,468 - +0.1
3,780,468 - -2.0
3,780,468 - -0.9
3,780,468 - +0.3
3,780,603 + RR42_RS17460 0.24 -0.3
3,780,603 + RR42_RS17460 0.24 -0.5
3,780,604 - RR42_RS17460 0.24 +0.4
3,780,727 - RR42_RS17460 0.44 -2.7
3,780,914 + RR42_RS17460 0.74 +0.5
3,780,915 - RR42_RS17460 0.74 -1.1
3,780,953 + RR42_RS17460 0.80 +0.3
3,781,013 + -1.6
3,781,013 + +1.0
3,781,014 - +0.4
3,781,014 - +0.8

Or see this region's nucleotide sequence