Experiment: R2A with Lomefloxacin 0.00075 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS17350 and RR42_RS17355 are separated by 62 nucleotides RR42_RS17355 and RR42_RS17360 are separated by 55 nucleotides RR42_RS17360 and RR42_RS17365 are separated by 65 nucleotides
RR42_RS17350: RR42_RS17350 - twitching motility protein PilT, at 3,755,168 to 3,756,211
_RS17350
RR42_RS17355: RR42_RS17355 - hypothetical protein, at 3,756,274 to 3,757,002
_RS17355
RR42_RS17360: RR42_RS17360 - pyrroline-5-carboxylate reductase, at 3,757,058 to 3,757,882
_RS17360
RR42_RS17365: RR42_RS17365 - 4-hydroxybenzoate polyprenyltransferase, at 3,757,948 to 3,758,841
_RS17365
Position (kb)
3757
3758 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3756.058 kb on - strand, within RR42_RS17350 at 3756.058 kb on - strand, within RR42_RS17350 at 3756.058 kb on - strand, within RR42_RS17350 at 3756.058 kb on - strand, within RR42_RS17350 at 3756.058 kb on - strand, within RR42_RS17350 at 3756.113 kb on - strand at 3756.191 kb on + strand at 3756.191 kb on + strand at 3756.195 kb on - strand at 3756.213 kb on + strand at 3756.213 kb on + strand at 3756.213 kb on + strand at 3756.213 kb on + strand at 3756.214 kb on - strand at 3756.214 kb on - strand at 3756.214 kb on - strand at 3756.214 kb on - strand at 3756.215 kb on + strand at 3756.215 kb on + strand at 3756.238 kb on + strand at 3756.238 kb on + strand at 3756.249 kb on + strand at 3756.259 kb on + strand at 3756.273 kb on + strand at 3756.445 kb on + strand, within RR42_RS17355 at 3757.961 kb on + strand at 3757.961 kb on + strand at 3757.961 kb on + strand at 3757.961 kb on + strand at 3757.962 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM remove 3,756,058 - RR42_RS17350 0.85 -0.1 3,756,058 - RR42_RS17350 0.85 -0.7 3,756,058 - RR42_RS17350 0.85 +1.1 3,756,058 - RR42_RS17350 0.85 +1.4 3,756,058 - RR42_RS17350 0.85 +0.4 3,756,113 - +1.1 3,756,191 + -1.3 3,756,191 + -0.2 3,756,195 - -0.6 3,756,213 + -1.8 3,756,213 + +0.4 3,756,213 + +0.1 3,756,213 + -0.2 3,756,214 - -0.5 3,756,214 - +1.0 3,756,214 - +0.7 3,756,214 - -0.6 3,756,215 + -0.2 3,756,215 + -0.4 3,756,238 + +0.2 3,756,238 + -0.1 3,756,249 + -2.1 3,756,259 + +0.1 3,756,273 + +1.1 3,756,445 + RR42_RS17355 0.23 +3.4 3,757,961 + -0.0 3,757,961 + +0.1 3,757,961 + +0.8 3,757,961 + +0.0 3,757,962 - -0.2
Or see this region's nucleotide sequence