Experiment: R2A with Lomefloxacin 0.00075 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS17250 and RR42_RS17255 are separated by 13 nucleotides RR42_RS17255 and RR42_RS17260 are separated by 53 nucleotides RR42_RS17260 and RR42_RS17265 are separated by 9 nucleotides
RR42_RS17250: RR42_RS17250 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 3,734,113 to 3,734,934
_RS17250
RR42_RS17255: RR42_RS17255 - ABC transporter permease, at 3,734,948 to 3,735,646
_RS17255
RR42_RS17260: RR42_RS17260 - ABC transporter ATP-binding protein, at 3,735,700 to 3,736,374
_RS17260
RR42_RS17265: RR42_RS17265 - aminodeoxychorismate synthase, at 3,736,384 to 3,738,147
_RS17265
Position (kb)
3734
3735
3736 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3733.993 kb on + strand at 3735.089 kb on + strand, within RR42_RS17255 at 3735.090 kb on - strand, within RR42_RS17255 at 3735.090 kb on - strand, within RR42_RS17255 at 3735.188 kb on + strand, within RR42_RS17255 at 3735.238 kb on + strand, within RR42_RS17255 at 3735.485 kb on - strand, within RR42_RS17255 at 3735.556 kb on + strand, within RR42_RS17255 at 3735.557 kb on - strand, within RR42_RS17255 at 3735.741 kb on - strand at 3735.763 kb on - strand at 3735.961 kb on + strand, within RR42_RS17260 at 3735.961 kb on + strand, within RR42_RS17260 at 3736.015 kb on + strand, within RR42_RS17260 at 3736.015 kb on + strand, within RR42_RS17260 at 3736.411 kb on + strand at 3736.411 kb on + strand at 3736.411 kb on + strand at 3736.412 kb on - strand at 3736.441 kb on + strand at 3736.442 kb on - strand at 3736.442 kb on - strand at 3736.505 kb on + strand at 3736.506 kb on - strand at 3736.522 kb on + strand at 3736.522 kb on + strand at 3736.522 kb on + strand at 3736.523 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM remove 3,733,993 + +0.6 3,735,089 + RR42_RS17255 0.20 +0.6 3,735,090 - RR42_RS17255 0.20 +0.2 3,735,090 - RR42_RS17255 0.20 +0.4 3,735,188 + RR42_RS17255 0.34 -3.2 3,735,238 + RR42_RS17255 0.41 +0.3 3,735,485 - RR42_RS17255 0.77 +1.3 3,735,556 + RR42_RS17255 0.87 +0.4 3,735,557 - RR42_RS17255 0.87 +1.5 3,735,741 - -0.6 3,735,763 - -0.0 3,735,961 + RR42_RS17260 0.39 +1.6 3,735,961 + RR42_RS17260 0.39 +0.3 3,736,015 + RR42_RS17260 0.47 -1.0 3,736,015 + RR42_RS17260 0.47 +1.5 3,736,411 + +0.2 3,736,411 + -0.2 3,736,411 + +1.1 3,736,412 - -1.2 3,736,441 + +1.4 3,736,442 - +0.1 3,736,442 - -0.4 3,736,505 + -0.2 3,736,506 - +0.5 3,736,522 + -0.2 3,736,522 + -0.7 3,736,522 + -0.2 3,736,523 - +0.8
Or see this region's nucleotide sequence