Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS15670

Experiment: R2A with Lomefloxacin 0.00075 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS15660 and RR42_RS15665 are separated by 14 nucleotidesRR42_RS15665 and RR42_RS15670 are separated by 134 nucleotidesRR42_RS15670 and RR42_RS15675 are separated by 97 nucleotides RR42_RS15660: RR42_RS15660 - protein-L-isoaspartate O-methyltransferase, at 3,383,508 to 3,384,161 _RS15660 RR42_RS15665: RR42_RS15665 - sulfurtransferase, at 3,384,176 to 3,384,499 _RS15665 RR42_RS15670: RR42_RS15670 - channel protein TolC, at 3,384,634 to 3,386,121 _RS15670 RR42_RS15675: RR42_RS15675 - heat-shock protein HslJ, at 3,386,219 to 3,387,055 _RS15675 Position (kb) 3384 3385 3386 3387Strain fitness (log2 ratio) -2 -1 0 1at 3384.184 kb on - strandat 3384.184 kb on - strandat 3384.252 kb on + strand, within RR42_RS15665at 3384.302 kb on - strand, within RR42_RS15665at 3384.355 kb on + strand, within RR42_RS15665at 3384.355 kb on + strand, within RR42_RS15665at 3384.356 kb on - strand, within RR42_RS15665at 3384.356 kb on - strand, within RR42_RS15665at 3384.356 kb on - strand, within RR42_RS15665at 3384.434 kb on + strand, within RR42_RS15665at 3384.435 kb on - strand, within RR42_RS15665at 3384.435 kb on - strand, within RR42_RS15665at 3384.494 kb on + strandat 3384.495 kb on - strandat 3384.707 kb on + strandat 3384.708 kb on - strandat 3384.752 kb on - strandat 3384.870 kb on - strand, within RR42_RS15670at 3384.936 kb on + strand, within RR42_RS15670at 3384.936 kb on + strand, within RR42_RS15670at 3384.937 kb on - strand, within RR42_RS15670at 3384.937 kb on - strand, within RR42_RS15670at 3384.937 kb on - strand, within RR42_RS15670at 3384.992 kb on - strand, within RR42_RS15670at 3384.992 kb on - strand, within RR42_RS15670at 3384.992 kb on - strand, within RR42_RS15670at 3384.992 kb on - strand, within RR42_RS15670at 3385.060 kb on - strand, within RR42_RS15670at 3385.078 kb on + strand, within RR42_RS15670at 3385.112 kb on - strand, within RR42_RS15670at 3385.223 kb on - strand, within RR42_RS15670at 3385.223 kb on - strand, within RR42_RS15670at 3385.427 kb on - strand, within RR42_RS15670at 3385.454 kb on - strand, within RR42_RS15670at 3385.562 kb on - strand, within RR42_RS15670at 3385.728 kb on - strand, within RR42_RS15670at 3386.005 kb on + strandat 3386.005 kb on + strandat 3386.322 kb on + strand, within RR42_RS15675at 3386.457 kb on + strand, within RR42_RS15675at 3386.457 kb on + strand, within RR42_RS15675at 3386.457 kb on + strand, within RR42_RS15675at 3386.457 kb on + strand, within RR42_RS15675at 3386.737 kb on - strand, within RR42_RS15675at 3386.746 kb on + strand, within RR42_RS15675at 3387.044 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM
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3,384,184 - +0.5
3,384,184 - +1.3
3,384,252 + RR42_RS15665 0.23 -1.5
3,384,302 - RR42_RS15665 0.39 -0.5
3,384,355 + RR42_RS15665 0.55 +0.8
3,384,355 + RR42_RS15665 0.55 +0.6
3,384,356 - RR42_RS15665 0.56 -0.4
3,384,356 - RR42_RS15665 0.56 -0.3
3,384,356 - RR42_RS15665 0.56 -0.9
3,384,434 + RR42_RS15665 0.80 -0.4
3,384,435 - RR42_RS15665 0.80 +1.6
3,384,435 - RR42_RS15665 0.80 -1.8
3,384,494 + +0.1
3,384,495 - +0.5
3,384,707 + -0.3
3,384,708 - +1.6
3,384,752 - -0.1
3,384,870 - RR42_RS15670 0.16 -0.4
3,384,936 + RR42_RS15670 0.20 -0.4
3,384,936 + RR42_RS15670 0.20 -0.3
3,384,937 - RR42_RS15670 0.20 +0.5
3,384,937 - RR42_RS15670 0.20 -0.4
3,384,937 - RR42_RS15670 0.20 -0.7
3,384,992 - RR42_RS15670 0.24 -0.7
3,384,992 - RR42_RS15670 0.24 +1.0
3,384,992 - RR42_RS15670 0.24 +1.0
3,384,992 - RR42_RS15670 0.24 +0.2
3,385,060 - RR42_RS15670 0.29 +0.7
3,385,078 + RR42_RS15670 0.30 +1.6
3,385,112 - RR42_RS15670 0.32 +1.7
3,385,223 - RR42_RS15670 0.40 -0.2
3,385,223 - RR42_RS15670 0.40 +0.5
3,385,427 - RR42_RS15670 0.53 +0.8
3,385,454 - RR42_RS15670 0.55 -0.3
3,385,562 - RR42_RS15670 0.62 -0.4
3,385,728 - RR42_RS15670 0.74 +0.8
3,386,005 + -1.4
3,386,005 + +0.1
3,386,322 + RR42_RS15675 0.12 -0.3
3,386,457 + RR42_RS15675 0.28 +0.7
3,386,457 + RR42_RS15675 0.28 +0.1
3,386,457 + RR42_RS15675 0.28 +0.1
3,386,457 + RR42_RS15675 0.28 -2.1
3,386,737 - RR42_RS15675 0.62 -0.4
3,386,746 + RR42_RS15675 0.63 -1.5
3,387,044 + -0.5

Or see this region's nucleotide sequence