Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS12530

Experiment: R2A with Lomefloxacin 0.00075 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS12515 and RR42_RS12520 are separated by 56 nucleotidesRR42_RS12520 and RR42_RS12525 are separated by 1 nucleotidesRR42_RS12525 and RR42_RS12530 are separated by 14 nucleotidesRR42_RS12530 and RR42_RS12535 are separated by 207 nucleotides RR42_RS12515: RR42_RS12515 - camphor resistance protein CrcB, at 2,691,125 to 2,691,505 _RS12515 RR42_RS12520: RR42_RS12520 - molybdenum cofactor biosynthesis protein MoaE, at 2,691,562 to 2,692,032 _RS12520 RR42_RS12525: RR42_RS12525 - molybdenum cofactor biosynthesis protein MoaD, at 2,692,034 to 2,692,291 _RS12525 RR42_RS12530: RR42_RS12530 - molybdenum cofactor biosynthesis protein MoaA, at 2,692,306 to 2,693,547 _RS12530 RR42_RS12535: RR42_RS12535 - threonine synthase, at 2,693,755 to 2,695,203 _RS12535 Position (kb) 2692 2693 2694Strain fitness (log2 ratio) -1 0 1 2 3at 2691.689 kb on + strand, within RR42_RS12520at 2691.690 kb on - strand, within RR42_RS12520at 2691.690 kb on - strand, within RR42_RS12520at 2691.875 kb on + strand, within RR42_RS12520at 2691.875 kb on + strand, within RR42_RS12520at 2691.979 kb on + strand, within RR42_RS12520at 2691.979 kb on + strand, within RR42_RS12520at 2691.980 kb on - strand, within RR42_RS12520at 2692.019 kb on - strandat 2692.091 kb on - strand, within RR42_RS12525at 2692.307 kb on + strandat 2692.308 kb on - strandat 2692.344 kb on + strandat 2692.344 kb on + strandat 2692.344 kb on + strandat 2692.599 kb on + strand, within RR42_RS12530at 2692.600 kb on - strand, within RR42_RS12530at 2692.600 kb on - strand, within RR42_RS12530at 2692.752 kb on + strand, within RR42_RS12530at 2692.752 kb on + strand, within RR42_RS12530at 2692.752 kb on + strand, within RR42_RS12530at 2692.753 kb on - strand, within RR42_RS12530at 2692.753 kb on - strand, within RR42_RS12530at 2692.753 kb on - strand, within RR42_RS12530at 2692.753 kb on - strand, within RR42_RS12530at 2692.928 kb on + strand, within RR42_RS12530at 2692.928 kb on + strand, within RR42_RS12530at 2692.929 kb on - strand, within RR42_RS12530at 2693.574 kb on + strandat 2693.583 kb on + strandat 2693.583 kb on + strandat 2693.583 kb on + strandat 2693.584 kb on - strandat 2693.584 kb on - strandat 2693.584 kb on - strandat 2693.584 kb on - strandat 2693.584 kb on - strandat 2693.706 kb on + strandat 2693.706 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM
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2,691,689 + RR42_RS12520 0.27 +1.6
2,691,690 - RR42_RS12520 0.27 +0.3
2,691,690 - RR42_RS12520 0.27 +1.9
2,691,875 + RR42_RS12520 0.66 +1.2
2,691,875 + RR42_RS12520 0.66 +0.7
2,691,979 + RR42_RS12520 0.89 +1.2
2,691,979 + RR42_RS12520 0.89 +1.6
2,691,980 - RR42_RS12520 0.89 -0.2
2,692,019 - +1.9
2,692,091 - RR42_RS12525 0.22 +1.3
2,692,307 + +2.9
2,692,308 - +0.3
2,692,344 + -0.3
2,692,344 + +1.3
2,692,344 + +0.0
2,692,599 + RR42_RS12530 0.24 +0.7
2,692,600 - RR42_RS12530 0.24 +0.9
2,692,600 - RR42_RS12530 0.24 +0.6
2,692,752 + RR42_RS12530 0.36 +2.0
2,692,752 + RR42_RS12530 0.36 +0.3
2,692,752 + RR42_RS12530 0.36 +0.7
2,692,753 - RR42_RS12530 0.36 +0.5
2,692,753 - RR42_RS12530 0.36 +0.9
2,692,753 - RR42_RS12530 0.36 +0.5
2,692,753 - RR42_RS12530 0.36 +0.7
2,692,928 + RR42_RS12530 0.50 +0.8
2,692,928 + RR42_RS12530 0.50 +1.3
2,692,929 - RR42_RS12530 0.50 +1.0
2,693,574 + +0.6
2,693,583 + +1.9
2,693,583 + +2.3
2,693,583 + +2.2
2,693,584 - +0.6
2,693,584 - +0.8
2,693,584 - -0.3
2,693,584 - +0.1
2,693,584 - -0.6
2,693,706 + +0.4
2,693,706 + +0.2

Or see this region's nucleotide sequence