Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS11435

Experiment: R2A with Lomefloxacin 0.00075 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS11425 and RR42_RS11430 are separated by 386 nucleotidesRR42_RS11430 and RR42_RS11435 are separated by 146 nucleotidesRR42_RS11435 and RR42_RS11440 are separated by 89 nucleotidesRR42_RS11440 and RR42_RS11445 are separated by 261 nucleotides RR42_RS11425: RR42_RS11425 - MFS transporter, at 2,445,462 to 2,445,899 _RS11425 RR42_RS11430: RR42_RS11430 - hypothetical protein, at 2,446,286 to 2,446,492 _RS11430 RR42_RS11435: RR42_RS11435 - ABC transporter permease, at 2,446,639 to 2,447,532 _RS11435 RR42_RS11440: RR42_RS11440 - cytochrome Cbb3, at 2,447,622 to 2,448,101 _RS11440 RR42_RS11445: RR42_RS11445 - Fis family transcriptional regulator, at 2,448,363 to 2,450,402 _RS11445 Position (kb) 2446 2447 2448Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2445.682 kb on + strand, within RR42_RS11425at 2445.759 kb on - strand, within RR42_RS11425at 2445.809 kb on - strand, within RR42_RS11425at 2445.877 kb on + strandat 2445.878 kb on - strandat 2446.002 kb on - strandat 2446.002 kb on - strandat 2446.002 kb on - strandat 2446.149 kb on + strandat 2446.149 kb on + strandat 2446.150 kb on - strandat 2446.150 kb on - strandat 2446.150 kb on - strandat 2446.251 kb on + strandat 2446.252 kb on - strandat 2446.579 kb on + strandat 2446.580 kb on - strandat 2446.624 kb on + strandat 2446.625 kb on - strandat 2446.625 kb on - strandat 2446.769 kb on - strand, within RR42_RS11435at 2446.862 kb on + strand, within RR42_RS11435at 2446.863 kb on - strand, within RR42_RS11435at 2446.938 kb on - strand, within RR42_RS11435at 2447.041 kb on + strand, within RR42_RS11435at 2447.102 kb on + strand, within RR42_RS11435at 2447.103 kb on - strand, within RR42_RS11435at 2447.186 kb on + strand, within RR42_RS11435at 2447.196 kb on - strand, within RR42_RS11435at 2447.367 kb on - strand, within RR42_RS11435at 2447.644 kb on + strandat 2447.645 kb on - strandat 2447.645 kb on - strandat 2447.645 kb on - strandat 2447.701 kb on + strand, within RR42_RS11440at 2447.701 kb on + strand, within RR42_RS11440at 2447.702 kb on - strand, within RR42_RS11440at 2447.702 kb on - strand, within RR42_RS11440at 2447.702 kb on - strand, within RR42_RS11440at 2447.702 kb on - strand, within RR42_RS11440at 2447.702 kb on - strand, within RR42_RS11440at 2447.721 kb on - strand, within RR42_RS11440at 2447.749 kb on + strand, within RR42_RS11440at 2447.750 kb on - strand, within RR42_RS11440at 2447.890 kb on + strand, within RR42_RS11440at 2447.906 kb on + strand, within RR42_RS11440at 2447.941 kb on + strand, within RR42_RS11440at 2447.941 kb on + strand, within RR42_RS11440at 2447.941 kb on + strand, within RR42_RS11440at 2447.949 kb on - strand, within RR42_RS11440at 2447.980 kb on - strand, within RR42_RS11440at 2448.484 kb on - strandat 2448.499 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM
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2,445,682 + RR42_RS11425 0.50 +0.4
2,445,759 - RR42_RS11425 0.68 -1.0
2,445,809 - RR42_RS11425 0.79 +2.7
2,445,877 + +0.1
2,445,878 - -0.0
2,446,002 - -1.1
2,446,002 - -0.4
2,446,002 - +0.8
2,446,149 + +0.5
2,446,149 + -0.4
2,446,150 - +0.9
2,446,150 - -0.3
2,446,150 - -0.4
2,446,251 + -0.4
2,446,252 - -0.5
2,446,579 + +0.5
2,446,580 - +0.5
2,446,624 + +0.4
2,446,625 - +0.3
2,446,625 - -0.2
2,446,769 - RR42_RS11435 0.15 -0.6
2,446,862 + RR42_RS11435 0.25 +0.3
2,446,863 - RR42_RS11435 0.25 +0.2
2,446,938 - RR42_RS11435 0.33 -0.5
2,447,041 + RR42_RS11435 0.45 -0.0
2,447,102 + RR42_RS11435 0.52 -1.0
2,447,103 - RR42_RS11435 0.52 -0.9
2,447,186 + RR42_RS11435 0.61 -1.1
2,447,196 - RR42_RS11435 0.62 -1.8
2,447,367 - RR42_RS11435 0.81 -1.1
2,447,644 + +0.4
2,447,645 - +0.2
2,447,645 - +1.3
2,447,645 - +1.5
2,447,701 + RR42_RS11440 0.16 +0.1
2,447,701 + RR42_RS11440 0.16 -1.6
2,447,702 - RR42_RS11440 0.17 -0.8
2,447,702 - RR42_RS11440 0.17 -1.0
2,447,702 - RR42_RS11440 0.17 -0.7
2,447,702 - RR42_RS11440 0.17 +0.6
2,447,702 - RR42_RS11440 0.17 +1.2
2,447,721 - RR42_RS11440 0.21 -0.4
2,447,749 + RR42_RS11440 0.26 +0.2
2,447,750 - RR42_RS11440 0.27 -0.3
2,447,890 + RR42_RS11440 0.56 -0.2
2,447,906 + RR42_RS11440 0.59 -0.2
2,447,941 + RR42_RS11440 0.66 -0.5
2,447,941 + RR42_RS11440 0.66 +1.1
2,447,941 + RR42_RS11440 0.66 -1.4
2,447,949 - RR42_RS11440 0.68 -3.1
2,447,980 - RR42_RS11440 0.75 -0.1
2,448,484 - +1.3
2,448,499 - +0.7

Or see this region's nucleotide sequence