Experiment: R2A with Lomefloxacin 0.00075 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10555 and RR42_RS10560 are separated by 54 nucleotides RR42_RS10560 and RR42_RS10565 are separated by 39 nucleotides RR42_RS10565 and RR42_RS10570 are separated by 14 nucleotides
RR42_RS10555: RR42_RS10555 - helicase, at 2,256,305 to 2,256,703
_RS10555
RR42_RS10560: RR42_RS10560 - alpha/beta hydrolase, at 2,256,758 to 2,257,603
_RS10560
RR42_RS10565: RR42_RS10565 - alpha/beta hydrolase, at 2,257,643 to 2,258,611
_RS10565
RR42_RS10570: RR42_RS10570 - NADH:flavin oxidoreductase, at 2,258,626 to 2,259,702
_RS10570
Position (kb)
2257
2258
2259 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2256.876 kb on + strand, within RR42_RS10560 at 2256.877 kb on - strand, within RR42_RS10560 at 2256.877 kb on - strand, within RR42_RS10560 at 2256.877 kb on - strand, within RR42_RS10560 at 2256.990 kb on + strand, within RR42_RS10560 at 2256.990 kb on + strand, within RR42_RS10560 at 2256.991 kb on - strand, within RR42_RS10560 at 2257.018 kb on + strand, within RR42_RS10560 at 2257.125 kb on + strand, within RR42_RS10560 at 2257.722 kb on + strand at 2257.726 kb on + strand at 2257.726 kb on + strand at 2257.727 kb on - strand at 2258.044 kb on - strand, within RR42_RS10565 at 2258.044 kb on - strand, within RR42_RS10565 at 2258.424 kb on + strand, within RR42_RS10565 at 2258.424 kb on + strand, within RR42_RS10565 at 2258.675 kb on + strand at 2258.675 kb on + strand at 2258.676 kb on - strand at 2258.927 kb on + strand, within RR42_RS10570 at 2259.033 kb on + strand, within RR42_RS10570 at 2259.034 kb on - strand, within RR42_RS10570 at 2259.037 kb on + strand, within RR42_RS10570 at 2259.171 kb on - strand, within RR42_RS10570 at 2259.395 kb on - strand, within RR42_RS10570 at 2259.581 kb on + strand, within RR42_RS10570 at 2259.581 kb on + strand, within RR42_RS10570 at 2259.582 kb on - strand, within RR42_RS10570 at 2259.585 kb on - strand, within RR42_RS10570
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM remove 2,256,876 + RR42_RS10560 0.14 +0.4 2,256,877 - RR42_RS10560 0.14 +1.0 2,256,877 - RR42_RS10560 0.14 -0.6 2,256,877 - RR42_RS10560 0.14 -0.6 2,256,990 + RR42_RS10560 0.27 +0.3 2,256,990 + RR42_RS10560 0.27 +1.4 2,256,991 - RR42_RS10560 0.28 +0.3 2,257,018 + RR42_RS10560 0.31 +0.1 2,257,125 + RR42_RS10560 0.43 -0.5 2,257,722 + +0.2 2,257,726 + +0.3 2,257,726 + +0.4 2,257,727 - -0.5 2,258,044 - RR42_RS10565 0.41 +1.2 2,258,044 - RR42_RS10565 0.41 -1.0 2,258,424 + RR42_RS10565 0.81 -0.2 2,258,424 + RR42_RS10565 0.81 +1.8 2,258,675 + +0.5 2,258,675 + -0.8 2,258,676 - -0.5 2,258,927 + RR42_RS10570 0.28 -0.9 2,259,033 + RR42_RS10570 0.38 -0.6 2,259,034 - RR42_RS10570 0.38 -0.2 2,259,037 + RR42_RS10570 0.38 -2.2 2,259,171 - RR42_RS10570 0.51 -1.4 2,259,395 - RR42_RS10570 0.71 -0.6 2,259,581 + RR42_RS10570 0.89 +0.5 2,259,581 + RR42_RS10570 0.89 +1.4 2,259,582 - RR42_RS10570 0.89 -0.6 2,259,585 - RR42_RS10570 0.89 +1.0
Or see this region's nucleotide sequence