Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS08280

Experiment: R2A with Lomefloxacin 0.00075 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS08270 and RR42_RS08275 are separated by 20 nucleotidesRR42_RS08275 and RR42_RS08280 are separated by 9 nucleotidesRR42_RS08280 and RR42_RS08285 are separated by 73 nucleotides RR42_RS08270: RR42_RS08270 - hypothetical protein, at 1,753,964 to 1,754,614 _RS08270 RR42_RS08275: RR42_RS08275 - hypothetical protein, at 1,754,635 to 1,755,552 _RS08275 RR42_RS08280: RR42_RS08280 - hypothetical protein, at 1,755,562 to 1,755,762 _RS08280 RR42_RS08285: RR42_RS08285 - tail protein, at 1,755,836 to 1,756,330 _RS08285 Position (kb) 1755 1756Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1754.570 kb on - strandat 1754.570 kb on - strandat 1754.594 kb on - strandat 1754.594 kb on - strandat 1754.612 kb on + strandat 1754.656 kb on - strandat 1754.656 kb on - strandat 1754.832 kb on + strand, within RR42_RS08275at 1754.846 kb on - strand, within RR42_RS08275at 1754.993 kb on - strand, within RR42_RS08275at 1754.993 kb on - strand, within RR42_RS08275at 1755.096 kb on + strand, within RR42_RS08275at 1755.120 kb on + strand, within RR42_RS08275at 1755.120 kb on + strand, within RR42_RS08275at 1755.121 kb on - strand, within RR42_RS08275at 1755.379 kb on + strand, within RR42_RS08275at 1755.380 kb on - strand, within RR42_RS08275at 1755.380 kb on - strand, within RR42_RS08275at 1755.380 kb on - strand, within RR42_RS08275at 1755.424 kb on + strand, within RR42_RS08275at 1755.425 kb on - strand, within RR42_RS08275at 1755.548 kb on - strandat 1755.550 kb on + strandat 1755.550 kb on + strandat 1755.551 kb on - strandat 1755.551 kb on - strandat 1755.562 kb on - strandat 1755.667 kb on + strand, within RR42_RS08280at 1755.667 kb on + strand, within RR42_RS08280at 1755.779 kb on + strandat 1755.780 kb on - strandat 1755.821 kb on + strandat 1755.843 kb on - strandat 1755.843 kb on - strandat 1755.890 kb on + strand, within RR42_RS08285at 1755.988 kb on + strand, within RR42_RS08285at 1755.988 kb on + strand, within RR42_RS08285at 1755.988 kb on + strand, within RR42_RS08285at 1755.988 kb on + strand, within RR42_RS08285at 1755.988 kb on + strand, within RR42_RS08285at 1755.989 kb on - strand, within RR42_RS08285at 1756.094 kb on + strand, within RR42_RS08285at 1756.095 kb on - strand, within RR42_RS08285at 1756.259 kb on + strand, within RR42_RS08285at 1756.260 kb on - strand, within RR42_RS08285at 1756.260 kb on - strand, within RR42_RS08285at 1756.484 kb on + strandat 1756.636 kb on + strandat 1756.637 kb on - strandat 1756.637 kb on - strandat 1756.712 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM
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1,754,570 - +0.5
1,754,570 - +1.9
1,754,594 - +1.1
1,754,594 - +0.8
1,754,612 + +1.4
1,754,656 - +0.4
1,754,656 - +1.4
1,754,832 + RR42_RS08275 0.21 +1.5
1,754,846 - RR42_RS08275 0.23 +2.0
1,754,993 - RR42_RS08275 0.39 +1.8
1,754,993 - RR42_RS08275 0.39 +0.7
1,755,096 + RR42_RS08275 0.50 +1.0
1,755,120 + RR42_RS08275 0.53 +2.1
1,755,120 + RR42_RS08275 0.53 +2.3
1,755,121 - RR42_RS08275 0.53 +1.2
1,755,379 + RR42_RS08275 0.81 +0.2
1,755,380 - RR42_RS08275 0.81 +1.0
1,755,380 - RR42_RS08275 0.81 +0.6
1,755,380 - RR42_RS08275 0.81 +2.1
1,755,424 + RR42_RS08275 0.86 +1.5
1,755,425 - RR42_RS08275 0.86 +2.6
1,755,548 - +2.2
1,755,550 + +2.1
1,755,550 + +0.2
1,755,551 - +3.4
1,755,551 - +2.1
1,755,562 - -1.8
1,755,667 + RR42_RS08280 0.52 +2.0
1,755,667 + RR42_RS08280 0.52 +2.1
1,755,779 + -2.8
1,755,780 - +2.0
1,755,821 + +1.2
1,755,843 - +1.8
1,755,843 - +1.8
1,755,890 + RR42_RS08285 0.11 +1.0
1,755,988 + RR42_RS08285 0.31 +1.2
1,755,988 + RR42_RS08285 0.31 +1.8
1,755,988 + RR42_RS08285 0.31 +3.4
1,755,988 + RR42_RS08285 0.31 +2.2
1,755,988 + RR42_RS08285 0.31 +1.4
1,755,989 - RR42_RS08285 0.31 +0.6
1,756,094 + RR42_RS08285 0.52 +1.3
1,756,095 - RR42_RS08285 0.52 +0.7
1,756,259 + RR42_RS08285 0.85 +0.7
1,756,260 - RR42_RS08285 0.86 +2.1
1,756,260 - RR42_RS08285 0.86 +0.3
1,756,484 + +1.4
1,756,636 + +0.9
1,756,637 - +3.1
1,756,637 - -0.2
1,756,712 + +2.2

Or see this region's nucleotide sequence