Experiment: R2A with Lomefloxacin 0.00075 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05995 and RR42_RS06000 are separated by 100 nucleotides RR42_RS06000 and RR42_RS06005 are separated by 13 nucleotides RR42_RS06005 and RR42_RS06010 are separated by 213 nucleotides
RR42_RS05995: RR42_RS05995 - excinuclease ABC subunit B, at 1,318,179 to 1,320,281
_RS05995
RR42_RS06000: RR42_RS06000 - antibiotic biosynthesis monooxygenase, at 1,320,382 to 1,320,681
_RS06000
RR42_RS06005: RR42_RS06005 - hypothetical protein, at 1,320,695 to 1,321,030
_RS06005
RR42_RS06010: RR42_RS06010 - phosphotyrosine protein phosphatase, at 1,321,244 to 1,321,735
_RS06010
Position (kb)
1320
1321 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1319.431 kb on - strand, within RR42_RS05995 at 1319.431 kb on - strand, within RR42_RS05995 at 1319.676 kb on - strand, within RR42_RS05995 at 1320.033 kb on + strand, within RR42_RS05995 at 1320.033 kb on + strand, within RR42_RS05995 at 1320.034 kb on - strand, within RR42_RS05995 at 1320.034 kb on - strand, within RR42_RS05995 at 1320.095 kb on + strand at 1320.095 kb on + strand at 1320.096 kb on - strand at 1320.096 kb on - strand at 1320.096 kb on - strand at 1320.344 kb on + strand at 1320.345 kb on - strand at 1320.345 kb on - strand at 1320.473 kb on + strand, within RR42_RS06000 at 1320.474 kb on - strand, within RR42_RS06000 at 1321.040 kb on - strand at 1321.096 kb on - strand at 1321.254 kb on - strand at 1321.284 kb on + strand at 1321.284 kb on + strand at 1321.284 kb on + strand at 1321.284 kb on + strand at 1321.285 kb on - strand at 1321.285 kb on - strand at 1321.285 kb on - strand at 1321.382 kb on + strand, within RR42_RS06010 at 1321.383 kb on - strand, within RR42_RS06010 at 1321.383 kb on - strand, within RR42_RS06010 at 1321.383 kb on - strand, within RR42_RS06010 at 1321.440 kb on - strand, within RR42_RS06010 at 1321.628 kb on + strand, within RR42_RS06010 at 1321.632 kb on - strand, within RR42_RS06010 at 1321.667 kb on + strand, within RR42_RS06010 at 1321.667 kb on + strand, within RR42_RS06010 at 1321.668 kb on - strand, within RR42_RS06010 at 1321.668 kb on - strand, within RR42_RS06010 at 1321.668 kb on - strand, within RR42_RS06010
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM remove 1,319,431 - RR42_RS05995 0.60 +0.8 1,319,431 - RR42_RS05995 0.60 -0.3 1,319,676 - RR42_RS05995 0.71 -2.7 1,320,033 + RR42_RS05995 0.88 +0.3 1,320,033 + RR42_RS05995 0.88 +0.6 1,320,034 - RR42_RS05995 0.88 -0.7 1,320,034 - RR42_RS05995 0.88 -1.8 1,320,095 + -1.3 1,320,095 + -1.7 1,320,096 - +1.1 1,320,096 - +0.5 1,320,096 - -1.8 1,320,344 + +0.4 1,320,345 - +0.2 1,320,345 - -1.2 1,320,473 + RR42_RS06000 0.30 -1.8 1,320,474 - RR42_RS06000 0.31 +1.8 1,321,040 - -2.7 1,321,096 - -0.9 1,321,254 - +0.1 1,321,284 + -0.7 1,321,284 + -1.5 1,321,284 + +1.1 1,321,284 + +0.2 1,321,285 - -0.0 1,321,285 - -1.1 1,321,285 - +0.7 1,321,382 + RR42_RS06010 0.28 -1.4 1,321,383 - RR42_RS06010 0.28 +0.5 1,321,383 - RR42_RS06010 0.28 -0.7 1,321,383 - RR42_RS06010 0.28 +0.0 1,321,440 - RR42_RS06010 0.40 +0.2 1,321,628 + RR42_RS06010 0.78 +0.2 1,321,632 - RR42_RS06010 0.79 +1.2 1,321,667 + RR42_RS06010 0.86 -1.2 1,321,667 + RR42_RS06010 0.86 -0.3 1,321,668 - RR42_RS06010 0.86 +0.2 1,321,668 - RR42_RS06010 0.86 +0.9 1,321,668 - RR42_RS06010 0.86 -0.2
Or see this region's nucleotide sequence