Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS05925

Experiment: R2A with Lomefloxacin 0.00075 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS05920 and RR42_RS05925 are separated by 117 nucleotidesRR42_RS05925 and RR42_RS05930 are separated by 33 nucleotidesRR42_RS05930 and RR42_RS05935 are separated by 41 nucleotidesRR42_RS05935 and RR42_RS05940 are separated by 274 nucleotides RR42_RS05920: RR42_RS05920 - HrcA family transcriptional regulator, at 1,304,967 to 1,306,004 _RS05920 RR42_RS05925: RR42_RS05925 - ferrochelatase, at 1,306,122 to 1,307,237 _RS05925 RR42_RS05930: RR42_RS05930 - RNA-binding protein S4, at 1,307,271 to 1,307,666 _RS05930 RR42_RS05935: RR42_RS05935 - membrane protein, at 1,307,708 to 1,307,863 _RS05935 RR42_RS05940: RR42_RS05940 - co-chaperone GrpE, at 1,308,138 to 1,308,695 _RS05940 Position (kb) 1306 1307 1308Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1305.426 kb on - strand, within RR42_RS05920at 1305.480 kb on + strand, within RR42_RS05920at 1305.596 kb on + strand, within RR42_RS05920at 1305.596 kb on + strand, within RR42_RS05920at 1305.596 kb on + strand, within RR42_RS05920at 1305.596 kb on + strand, within RR42_RS05920at 1305.597 kb on - strand, within RR42_RS05920at 1305.876 kb on + strand, within RR42_RS05920at 1306.002 kb on + strandat 1306.002 kb on + strandat 1306.002 kb on + strandat 1306.003 kb on - strandat 1306.003 kb on - strandat 1306.003 kb on - strandat 1306.003 kb on - strandat 1306.003 kb on - strandat 1306.146 kb on + strandat 1307.278 kb on + strandat 1307.279 kb on - strandat 1307.279 kb on - strandat 1307.529 kb on + strand, within RR42_RS05930at 1307.530 kb on - strand, within RR42_RS05930at 1307.588 kb on + strand, within RR42_RS05930at 1307.588 kb on + strand, within RR42_RS05930at 1307.589 kb on - strand, within RR42_RS05930at 1307.730 kb on + strand, within RR42_RS05935at 1307.731 kb on - strand, within RR42_RS05935at 1307.731 kb on - strand, within RR42_RS05935at 1307.731 kb on - strand, within RR42_RS05935at 1307.731 kb on - strand, within RR42_RS05935at 1307.742 kb on - strand, within RR42_RS05935at 1307.760 kb on + strand, within RR42_RS05935at 1307.820 kb on - strand, within RR42_RS05935at 1307.857 kb on + strandat 1307.857 kb on + strandat 1307.857 kb on + strandat 1307.858 kb on - strandat 1307.858 kb on - strandat 1307.858 kb on - strandat 1307.858 kb on - strandat 1307.968 kb on + strandat 1307.968 kb on + strandat 1307.968 kb on + strandat 1307.968 kb on + strandat 1307.969 kb on - strandat 1307.969 kb on - strandat 1307.969 kb on - strandat 1307.969 kb on - strandat 1307.977 kb on + strandat 1307.978 kb on - strandat 1307.999 kb on + strandat 1308.070 kb on - strandat 1308.070 kb on - strandat 1308.071 kb on + strandat 1308.071 kb on + strandat 1308.071 kb on + strandat 1308.071 kb on + strandat 1308.071 kb on + strandat 1308.125 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM
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1,305,426 - RR42_RS05920 0.44 +0.5
1,305,480 + RR42_RS05920 0.49 +1.0
1,305,596 + RR42_RS05920 0.61 -0.1
1,305,596 + RR42_RS05920 0.61 +0.1
1,305,596 + RR42_RS05920 0.61 +0.4
1,305,596 + RR42_RS05920 0.61 -2.1
1,305,597 - RR42_RS05920 0.61 -1.6
1,305,876 + RR42_RS05920 0.88 -0.6
1,306,002 + -1.9
1,306,002 + -0.2
1,306,002 + -0.1
1,306,003 - -0.4
1,306,003 - -2.9
1,306,003 - -0.7
1,306,003 - -0.5
1,306,003 - +0.2
1,306,146 + -1.1
1,307,278 + -0.5
1,307,279 - -1.5
1,307,279 - +1.0
1,307,529 + RR42_RS05930 0.65 -0.3
1,307,530 - RR42_RS05930 0.65 +1.8
1,307,588 + RR42_RS05930 0.80 +0.3
1,307,588 + RR42_RS05930 0.80 -0.2
1,307,589 - RR42_RS05930 0.80 -0.0
1,307,730 + RR42_RS05935 0.14 +1.2
1,307,731 - RR42_RS05935 0.15 +0.0
1,307,731 - RR42_RS05935 0.15 +0.3
1,307,731 - RR42_RS05935 0.15 -0.6
1,307,731 - RR42_RS05935 0.15 +0.8
1,307,742 - RR42_RS05935 0.22 +1.5
1,307,760 + RR42_RS05935 0.33 -0.8
1,307,820 - RR42_RS05935 0.72 +2.8
1,307,857 + -0.8
1,307,857 + -0.1
1,307,857 + +0.0
1,307,858 - -0.7
1,307,858 - +0.8
1,307,858 - +0.7
1,307,858 - +1.0
1,307,968 + -0.1
1,307,968 + -0.3
1,307,968 + +0.5
1,307,968 + -1.0
1,307,969 - +0.4
1,307,969 - +0.5
1,307,969 - -0.9
1,307,969 - +0.6
1,307,977 + +0.1
1,307,978 - +0.4
1,307,999 + -0.8
1,308,070 - +1.5
1,308,070 - +0.9
1,308,071 + -1.1
1,308,071 + -0.6
1,308,071 + +0.3
1,308,071 + +1.2
1,308,071 + +0.3
1,308,125 + +0.5

Or see this region's nucleotide sequence