Experiment: R2A with Lomefloxacin 0.00075 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05880 and RR42_RS05885 are separated by 60 nucleotides RR42_RS05885 and RR42_RS05890 are separated by 258 nucleotides
RR42_RS05880: RR42_RS05880 - acyltransferase, at 1,290,819 to 1,291,676
_RS05880
RR42_RS05885: RR42_RS05885 - hypothetical protein, at 1,291,737 to 1,295,921
_RS05885
RR42_RS05890: RR42_RS05890 - glutamine-synthetase adenylyltransferase, at 1,296,180 to 1,299,140
_RS05890
Position (kb)
1291
1292
1293
1294
1295
1296 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1290.813 kb on + strand at 1290.813 kb on + strand at 1290.813 kb on + strand at 1290.813 kb on + strand at 1290.813 kb on + strand at 1290.814 kb on - strand at 1290.814 kb on - strand at 1290.814 kb on - strand at 1290.820 kb on + strand at 1290.820 kb on + strand at 1290.820 kb on + strand at 1290.821 kb on - strand at 1290.821 kb on - strand at 1290.821 kb on - strand at 1290.821 kb on - strand at 1290.821 kb on - strand at 1290.821 kb on - strand at 1291.015 kb on + strand, within RR42_RS05880 at 1291.015 kb on + strand, within RR42_RS05880 at 1291.015 kb on + strand, within RR42_RS05880 at 1291.016 kb on - strand, within RR42_RS05880 at 1291.016 kb on - strand, within RR42_RS05880 at 1291.016 kb on - strand, within RR42_RS05880 at 1291.016 kb on - strand, within RR42_RS05880 at 1291.016 kb on - strand, within RR42_RS05880 at 1291.078 kb on + strand, within RR42_RS05880 at 1291.079 kb on - strand, within RR42_RS05880 at 1291.079 kb on - strand, within RR42_RS05880 at 1291.162 kb on + strand, within RR42_RS05880 at 1291.162 kb on + strand, within RR42_RS05880 at 1291.163 kb on - strand, within RR42_RS05880 at 1291.163 kb on - strand, within RR42_RS05880 at 1291.232 kb on + strand, within RR42_RS05880 at 1291.502 kb on - strand, within RR42_RS05880 at 1291.756 kb on + strand at 1291.799 kb on + strand at 1291.800 kb on - strand at 1291.800 kb on - strand at 1291.800 kb on - strand at 1291.800 kb on - strand at 1291.847 kb on + strand at 1291.847 kb on + strand at 1291.847 kb on + strand at 1292.276 kb on + strand, within RR42_RS05885 at 1292.276 kb on + strand, within RR42_RS05885 at 1292.277 kb on - strand, within RR42_RS05885 at 1292.277 kb on - strand, within RR42_RS05885 at 1292.277 kb on - strand, within RR42_RS05885 at 1292.366 kb on + strand, within RR42_RS05885 at 1292.366 kb on + strand, within RR42_RS05885 at 1292.367 kb on - strand, within RR42_RS05885 at 1292.367 kb on - strand, within RR42_RS05885 at 1292.484 kb on - strand, within RR42_RS05885 at 1292.944 kb on - strand, within RR42_RS05885 at 1293.112 kb on + strand, within RR42_RS05885 at 1293.177 kb on + strand, within RR42_RS05885 at 1293.319 kb on - strand, within RR42_RS05885 at 1293.624 kb on - strand, within RR42_RS05885 at 1293.651 kb on - strand, within RR42_RS05885 at 1294.108 kb on + strand, within RR42_RS05885 at 1294.145 kb on - strand, within RR42_RS05885 at 1294.611 kb on + strand, within RR42_RS05885 at 1295.161 kb on + strand, within RR42_RS05885 at 1295.248 kb on + strand, within RR42_RS05885 at 1295.248 kb on + strand, within RR42_RS05885 at 1295.248 kb on + strand, within RR42_RS05885 at 1295.249 kb on - strand, within RR42_RS05885 at 1295.249 kb on - strand, within RR42_RS05885 at 1295.250 kb on - strand, within RR42_RS05885 at 1295.272 kb on - strand, within RR42_RS05885 at 1295.713 kb on + strand at 1295.713 kb on + strand at 1295.713 kb on + strand at 1295.714 kb on - strand at 1295.714 kb on - strand at 1295.714 kb on - strand at 1295.714 kb on - strand at 1295.717 kb on + strand at 1295.718 kb on - strand at 1296.090 kb on + strand at 1296.090 kb on + strand at 1296.091 kb on - strand at 1296.091 kb on - strand at 1296.091 kb on - strand at 1296.091 kb on - strand at 1296.094 kb on - strand at 1296.094 kb on - strand at 1296.173 kb on - strand at 1296.173 kb on - strand at 1296.372 kb on + strand at 1296.372 kb on + strand at 1296.373 kb on - strand at 1296.385 kb on - strand at 1296.385 kb on - strand at 1296.385 kb on - strand at 1296.393 kb on + strand at 1296.393 kb on + strand at 1296.394 kb on - strand at 1296.660 kb on + strand, within RR42_RS05890 at 1296.699 kb on + strand, within RR42_RS05890 at 1296.700 kb on - strand, within RR42_RS05890 at 1296.816 kb on + strand, within RR42_RS05890 at 1296.816 kb on + strand, within RR42_RS05890 at 1296.817 kb on - strand, within RR42_RS05890 at 1296.871 kb on - strand, within RR42_RS05890 at 1296.871 kb on - strand, within RR42_RS05890 at 1296.871 kb on - strand, within RR42_RS05890
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM remove 1,290,813 + +0.6 1,290,813 + -0.1 1,290,813 + -0.3 1,290,813 + +0.8 1,290,813 + +0.5 1,290,814 - +0.8 1,290,814 - -1.5 1,290,814 - +0.4 1,290,820 + -2.6 1,290,820 + -1.0 1,290,820 + -0.2 1,290,821 - +0.7 1,290,821 - +0.4 1,290,821 - +0.9 1,290,821 - +1.4 1,290,821 - +1.6 1,290,821 - +0.0 1,291,015 + RR42_RS05880 0.23 -0.4 1,291,015 + RR42_RS05880 0.23 -0.5 1,291,015 + RR42_RS05880 0.23 +0.2 1,291,016 - RR42_RS05880 0.23 +1.4 1,291,016 - RR42_RS05880 0.23 -0.6 1,291,016 - RR42_RS05880 0.23 +0.3 1,291,016 - RR42_RS05880 0.23 +0.7 1,291,016 - RR42_RS05880 0.23 +1.4 1,291,078 + RR42_RS05880 0.30 -0.3 1,291,079 - RR42_RS05880 0.30 +0.9 1,291,079 - RR42_RS05880 0.30 -1.5 1,291,162 + RR42_RS05880 0.40 -2.0 1,291,162 + RR42_RS05880 0.40 -1.6 1,291,163 - RR42_RS05880 0.40 +0.7 1,291,163 - RR42_RS05880 0.40 +0.3 1,291,232 + RR42_RS05880 0.48 -0.2 1,291,502 - RR42_RS05880 0.80 -0.1 1,291,756 + +1.2 1,291,799 + +0.5 1,291,800 - +0.9 1,291,800 - +0.4 1,291,800 - -0.8 1,291,800 - +0.0 1,291,847 + -0.2 1,291,847 + +1.6 1,291,847 + +0.1 1,292,276 + RR42_RS05885 0.13 +1.4 1,292,276 + RR42_RS05885 0.13 +0.7 1,292,277 - RR42_RS05885 0.13 +2.4 1,292,277 - RR42_RS05885 0.13 +1.3 1,292,277 - RR42_RS05885 0.13 +0.7 1,292,366 + RR42_RS05885 0.15 +2.5 1,292,366 + RR42_RS05885 0.15 +2.2 1,292,367 - RR42_RS05885 0.15 +2.4 1,292,367 - RR42_RS05885 0.15 +1.7 1,292,484 - RR42_RS05885 0.18 +1.4 1,292,944 - RR42_RS05885 0.29 +0.6 1,293,112 + RR42_RS05885 0.33 +2.4 1,293,177 + RR42_RS05885 0.34 +1.5 1,293,319 - RR42_RS05885 0.38 +0.7 1,293,624 - RR42_RS05885 0.45 +2.2 1,293,651 - RR42_RS05885 0.46 +1.7 1,294,108 + RR42_RS05885 0.57 +0.2 1,294,145 - RR42_RS05885 0.58 -0.3 1,294,611 + RR42_RS05885 0.69 -0.4 1,295,161 + RR42_RS05885 0.82 +2.5 1,295,248 + RR42_RS05885 0.84 -0.1 1,295,248 + RR42_RS05885 0.84 +1.9 1,295,248 + RR42_RS05885 0.84 +0.5 1,295,249 - RR42_RS05885 0.84 +0.0 1,295,249 - RR42_RS05885 0.84 +1.1 1,295,250 - RR42_RS05885 0.84 +1.1 1,295,272 - RR42_RS05885 0.84 +2.5 1,295,713 + +0.8 1,295,713 + +0.5 1,295,713 + +0.3 1,295,714 - +2.0 1,295,714 - +1.8 1,295,714 - +1.6 1,295,714 - +1.3 1,295,717 + +1.3 1,295,718 - +1.3 1,296,090 + -1.6 1,296,090 + -0.0 1,296,091 - -1.6 1,296,091 - -0.2 1,296,091 - +2.8 1,296,091 - -2.2 1,296,094 - -0.8 1,296,094 - +0.4 1,296,173 - +0.3 1,296,173 - +0.8 1,296,372 + -1.6 1,296,372 + -0.4 1,296,373 - -0.7 1,296,385 - +0.6 1,296,385 - -0.9 1,296,385 - -0.3 1,296,393 + -0.3 1,296,393 + -1.9 1,296,394 - +0.3 1,296,660 + RR42_RS05890 0.16 +0.4 1,296,699 + RR42_RS05890 0.18 +2.1 1,296,700 - RR42_RS05890 0.18 -0.5 1,296,816 + RR42_RS05890 0.21 -0.7 1,296,816 + RR42_RS05890 0.21 +0.0 1,296,817 - RR42_RS05890 0.22 -0.4 1,296,871 - RR42_RS05890 0.23 -1.1 1,296,871 - RR42_RS05890 0.23 -0.1 1,296,871 - RR42_RS05890 0.23 -0.3
Or see this region's nucleotide sequence