Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS05615

Experiment: R2A with Lomefloxacin 0.00075 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS05605 and RR42_RS05610 are separated by 83 nucleotidesRR42_RS05610 and RR42_RS05615 are separated by 59 nucleotidesRR42_RS05615 and RR42_RS05620 are separated by 9 nucleotidesRR42_RS05620 and RR42_RS05625 are separated by 181 nucleotides RR42_RS05605: RR42_RS05605 - dehydratase, at 1,234,386 to 1,234,835 _RS05605 RR42_RS05610: RR42_RS05610 - dehydratase, at 1,234,919 to 1,235,398 _RS05610 RR42_RS05615: RR42_RS05615 - MFS transporter, at 1,235,458 to 1,236,759 _RS05615 RR42_RS05620: RR42_RS05620 - esterase, at 1,236,769 to 1,237,527 _RS05620 RR42_RS05625: RR42_RS05625 - 4-hydroxyacetophenone monooxygenase, at 1,237,709 to 1,239,196 _RS05625 Position (kb) 1235 1236 1237Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1234.485 kb on + strand, within RR42_RS05605at 1234.486 kb on - strand, within RR42_RS05605at 1234.486 kb on - strand, within RR42_RS05605at 1234.486 kb on - strand, within RR42_RS05605at 1234.486 kb on - strand, within RR42_RS05605at 1234.536 kb on + strand, within RR42_RS05605at 1234.536 kb on + strand, within RR42_RS05605at 1234.537 kb on - strand, within RR42_RS05605at 1234.537 kb on - strand, within RR42_RS05605at 1234.668 kb on + strand, within RR42_RS05605at 1234.668 kb on + strand, within RR42_RS05605at 1234.815 kb on + strandat 1234.815 kb on + strandat 1234.815 kb on + strandat 1234.816 kb on - strandat 1234.816 kb on - strandat 1234.816 kb on - strandat 1234.816 kb on - strandat 1234.816 kb on - strandat 1234.884 kb on + strandat 1234.884 kb on + strandat 1234.884 kb on + strandat 1234.885 kb on - strandat 1234.885 kb on - strandat 1234.893 kb on + strandat 1234.893 kb on + strandat 1234.894 kb on - strandat 1234.894 kb on - strandat 1234.902 kb on + strandat 1234.902 kb on + strandat 1234.925 kb on - strandat 1235.187 kb on - strand, within RR42_RS05610at 1235.209 kb on + strand, within RR42_RS05610at 1235.397 kb on - strandat 1235.397 kb on - strandat 1235.623 kb on + strand, within RR42_RS05615at 1235.835 kb on - strand, within RR42_RS05615at 1235.872 kb on + strand, within RR42_RS05615at 1235.872 kb on + strand, within RR42_RS05615at 1235.872 kb on + strand, within RR42_RS05615at 1236.063 kb on - strand, within RR42_RS05615at 1236.123 kb on - strand, within RR42_RS05615at 1236.271 kb on + strand, within RR42_RS05615at 1236.272 kb on - strand, within RR42_RS05615at 1236.292 kb on + strand, within RR42_RS05615at 1236.313 kb on + strand, within RR42_RS05615at 1236.314 kb on - strand, within RR42_RS05615at 1236.740 kb on + strandat 1236.740 kb on + strandat 1236.740 kb on + strandat 1236.741 kb on - strandat 1236.753 kb on + strandat 1236.902 kb on + strand, within RR42_RS05620at 1236.902 kb on + strand, within RR42_RS05620at 1237.323 kb on - strand, within RR42_RS05620at 1237.711 kb on - strandat 1237.711 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM
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1,234,485 + RR42_RS05605 0.22 -0.3
1,234,486 - RR42_RS05605 0.22 -0.1
1,234,486 - RR42_RS05605 0.22 -1.8
1,234,486 - RR42_RS05605 0.22 -0.8
1,234,486 - RR42_RS05605 0.22 +0.1
1,234,536 + RR42_RS05605 0.33 -1.3
1,234,536 + RR42_RS05605 0.33 +1.7
1,234,537 - RR42_RS05605 0.34 -0.1
1,234,537 - RR42_RS05605 0.34 +0.3
1,234,668 + RR42_RS05605 0.63 +0.4
1,234,668 + RR42_RS05605 0.63 -0.8
1,234,815 + +0.9
1,234,815 + -0.3
1,234,815 + -0.2
1,234,816 - +0.2
1,234,816 - +2.8
1,234,816 - -0.1
1,234,816 - -1.0
1,234,816 - +0.3
1,234,884 + +0.8
1,234,884 + +0.7
1,234,884 + +0.3
1,234,885 - +0.2
1,234,885 - -0.2
1,234,893 + -0.5
1,234,893 + -1.1
1,234,894 - -0.1
1,234,894 - +0.1
1,234,902 + +0.7
1,234,902 + +1.0
1,234,925 - +1.2
1,235,187 - RR42_RS05610 0.56 -0.8
1,235,209 + RR42_RS05610 0.60 -0.6
1,235,397 - +0.5
1,235,397 - +0.9
1,235,623 + RR42_RS05615 0.13 -0.8
1,235,835 - RR42_RS05615 0.29 +0.8
1,235,872 + RR42_RS05615 0.32 -0.9
1,235,872 + RR42_RS05615 0.32 -0.3
1,235,872 + RR42_RS05615 0.32 +0.0
1,236,063 - RR42_RS05615 0.46 -0.4
1,236,123 - RR42_RS05615 0.51 +0.4
1,236,271 + RR42_RS05615 0.62 -1.1
1,236,272 - RR42_RS05615 0.63 -0.4
1,236,292 + RR42_RS05615 0.64 -0.8
1,236,313 + RR42_RS05615 0.66 -1.8
1,236,314 - RR42_RS05615 0.66 +0.0
1,236,740 + -0.2
1,236,740 + +1.8
1,236,740 + -0.8
1,236,741 - -0.1
1,236,753 + -0.1
1,236,902 + RR42_RS05620 0.18 -0.6
1,236,902 + RR42_RS05620 0.18 -0.4
1,237,323 - RR42_RS05620 0.73 -1.1
1,237,711 - -1.3
1,237,711 - +0.1

Or see this region's nucleotide sequence