Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS01230

Experiment: R2A with Lomefloxacin 0.00075 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS01220 and RR42_RS01225 are separated by 30 nucleotidesRR42_RS01225 and RR42_RS01230 are separated by 184 nucleotidesRR42_RS01230 and RR42_RS01235 are separated by 86 nucleotidesRR42_RS01235 and RR42_RS01240 are separated by 122 nucleotides RR42_RS01220: RR42_RS01220 - exodeoxyribonuclease III, at 276,970 to 277,746 _RS01220 RR42_RS01225: RR42_RS01225 - orotate phosphoribosyltransferase, at 277,777 to 278,466 _RS01225 RR42_RS01230: RR42_RS01230 - acetoacetate decarboxylase, at 278,651 to 279,391 _RS01230 RR42_RS01235: RR42_RS01235 - 3-hydroxybutyrate dehydrogenase, at 279,478 to 280,260 _RS01235 RR42_RS01240: RR42_RS01240 - membrane protein, at 280,383 to 281,735 _RS01240 Position (kb) 278 279 280Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 277.734 kb on + strandat 278.696 kb on - strandat 278.831 kb on + strand, within RR42_RS01230at 278.832 kb on - strand, within RR42_RS01230at 278.873 kb on + strand, within RR42_RS01230at 278.874 kb on - strand, within RR42_RS01230at 278.874 kb on - strand, within RR42_RS01230at 278.906 kb on + strand, within RR42_RS01230at 278.906 kb on + strand, within RR42_RS01230at 278.927 kb on + strand, within RR42_RS01230at 278.927 kb on + strand, within RR42_RS01230at 278.927 kb on + strand, within RR42_RS01230at 278.928 kb on - strand, within RR42_RS01230at 278.928 kb on - strand, within RR42_RS01230at 278.942 kb on + strand, within RR42_RS01230at 278.943 kb on - strand, within RR42_RS01230at 278.983 kb on - strand, within RR42_RS01230at 278.983 kb on - strand, within RR42_RS01230at 279.034 kb on + strand, within RR42_RS01230at 279.034 kb on + strand, within RR42_RS01230at 279.056 kb on + strand, within RR42_RS01230at 279.092 kb on + strand, within RR42_RS01230at 279.092 kb on + strand, within RR42_RS01230at 279.152 kb on + strand, within RR42_RS01230at 279.153 kb on - strand, within RR42_RS01230at 279.212 kb on + strand, within RR42_RS01230at 279.212 kb on + strand, within RR42_RS01230at 279.212 kb on + strand, within RR42_RS01230at 279.213 kb on - strand, within RR42_RS01230at 279.213 kb on - strand, within RR42_RS01230at 279.335 kb on + strandat 279.336 kb on - strandat 279.355 kb on - strandat 279.380 kb on + strandat 279.778 kb on + strand, within RR42_RS01235at 279.858 kb on + strand, within RR42_RS01235at 279.858 kb on + strand, within RR42_RS01235at 279.858 kb on + strand, within RR42_RS01235at 279.858 kb on + strand, within RR42_RS01235at 279.858 kb on + strand, within RR42_RS01235at 279.859 kb on - strand, within RR42_RS01235at 279.859 kb on - strand, within RR42_RS01235at 279.859 kb on - strand, within RR42_RS01235at 279.859 kb on - strand, within RR42_RS01235at 279.859 kb on - strand, within RR42_RS01235at 279.859 kb on - strand, within RR42_RS01235at 279.859 kb on - strand, within RR42_RS01235at 279.859 kb on - strand, within RR42_RS01235at 279.859 kb on - strand, within RR42_RS01235at 279.862 kb on + strand, within RR42_RS01235at 279.862 kb on + strand, within RR42_RS01235at 279.863 kb on - strand, within RR42_RS01235at 279.863 kb on - strand, within RR42_RS01235at 279.863 kb on - strand, within RR42_RS01235at 279.892 kb on + strand, within RR42_RS01235at 279.892 kb on + strand, within RR42_RS01235at 279.893 kb on - strand, within RR42_RS01235at 279.893 kb on - strand, within RR42_RS01235at 279.893 kb on - strand, within RR42_RS01235at 279.940 kb on + strand, within RR42_RS01235at 279.940 kb on + strand, within RR42_RS01235at 279.941 kb on - strand, within RR42_RS01235at 280.004 kb on - strand, within RR42_RS01235at 280.004 kb on - strand, within RR42_RS01235at 280.231 kb on - strandat 280.249 kb on + strandat 280.249 kb on + strandat 280.249 kb on + strandat 280.250 kb on - strandat 280.250 kb on - strandat 280.259 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM
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277,734 + +0.3
278,696 - +0.2
278,831 + RR42_RS01230 0.24 +0.7
278,832 - RR42_RS01230 0.24 +0.2
278,873 + RR42_RS01230 0.30 +0.5
278,874 - RR42_RS01230 0.30 +0.2
278,874 - RR42_RS01230 0.30 -0.1
278,906 + RR42_RS01230 0.34 +0.6
278,906 + RR42_RS01230 0.34 +0.8
278,927 + RR42_RS01230 0.37 +1.9
278,927 + RR42_RS01230 0.37 -0.9
278,927 + RR42_RS01230 0.37 -1.5
278,928 - RR42_RS01230 0.37 +1.0
278,928 - RR42_RS01230 0.37 +0.2
278,942 + RR42_RS01230 0.39 +0.2
278,943 - RR42_RS01230 0.39 -0.3
278,983 - RR42_RS01230 0.45 +0.3
278,983 - RR42_RS01230 0.45 +0.6
279,034 + RR42_RS01230 0.52 +0.6
279,034 + RR42_RS01230 0.52 +0.1
279,056 + RR42_RS01230 0.55 +1.3
279,092 + RR42_RS01230 0.60 -1.4
279,092 + RR42_RS01230 0.60 -0.4
279,152 + RR42_RS01230 0.68 +0.5
279,153 - RR42_RS01230 0.68 +0.0
279,212 + RR42_RS01230 0.76 +0.9
279,212 + RR42_RS01230 0.76 -0.9
279,212 + RR42_RS01230 0.76 +0.1
279,213 - RR42_RS01230 0.76 -0.4
279,213 - RR42_RS01230 0.76 -2.9
279,335 + -1.1
279,336 - -0.1
279,355 - -0.7
279,380 + +0.9
279,778 + RR42_RS01235 0.38 +0.3
279,858 + RR42_RS01235 0.49 +0.1
279,858 + RR42_RS01235 0.49 -0.3
279,858 + RR42_RS01235 0.49 -0.9
279,858 + RR42_RS01235 0.49 +0.8
279,858 + RR42_RS01235 0.49 +0.1
279,859 - RR42_RS01235 0.49 -0.1
279,859 - RR42_RS01235 0.49 -2.0
279,859 - RR42_RS01235 0.49 -0.7
279,859 - RR42_RS01235 0.49 +2.3
279,859 - RR42_RS01235 0.49 +0.2
279,859 - RR42_RS01235 0.49 -0.8
279,859 - RR42_RS01235 0.49 +0.3
279,859 - RR42_RS01235 0.49 +0.4
279,859 - RR42_RS01235 0.49 +0.3
279,862 + RR42_RS01235 0.49 +0.4
279,862 + RR42_RS01235 0.49 -1.2
279,863 - RR42_RS01235 0.49 -2.2
279,863 - RR42_RS01235 0.49 -0.1
279,863 - RR42_RS01235 0.49 -0.5
279,892 + RR42_RS01235 0.53 -0.4
279,892 + RR42_RS01235 0.53 -0.1
279,893 - RR42_RS01235 0.53 +0.5
279,893 - RR42_RS01235 0.53 +1.7
279,893 - RR42_RS01235 0.53 -1.9
279,940 + RR42_RS01235 0.59 +0.7
279,940 + RR42_RS01235 0.59 -0.1
279,941 - RR42_RS01235 0.59 +0.9
280,004 - RR42_RS01235 0.67 +0.3
280,004 - RR42_RS01235 0.67 -0.2
280,231 - +0.8
280,249 + -1.3
280,249 + +0.5
280,249 + +0.8
280,250 - +0.4
280,250 - +0.1
280,259 - +0.3

Or see this region's nucleotide sequence