Experiment: R2A with Lomefloxacin 0.00075 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00605 and RR42_RS00610 are separated by 169 nucleotides RR42_RS00610 and RR42_RS00615 are separated by 35 nucleotides RR42_RS00615 and RR42_RS00620 overlap by 4 nucleotides
RR42_RS00605: RR42_RS00605 - glutamyl-tRNA amidotransferase, at 158,952 to 160,412
_RS00605
RR42_RS00610: RR42_RS00610 - hypothetical protein, at 160,582 to 161,250
_RS00610
RR42_RS00615: RR42_RS00615 - recombinase XerC, at 161,286 to 162,410
_RS00615
RR42_RS00620: RR42_RS00620 - XRE family transcriptional regulator, at 162,407 to 163,366
_RS00620
Position (kb) 161
162
163 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 160.410 kb on + strand at 160.420 kb on - strand at 160.457 kb on + strand at 160.458 kb on - strand at 160.550 kb on - strand at 160.606 kb on + strand at 160.606 kb on + strand at 160.607 kb on - strand at 160.861 kb on + strand at 160.862 kb on - strand, within RR42_RS00610 at 160.939 kb on + strand, within RR42_RS00610 at 160.939 kb on + strand, within RR42_RS00610 at 160.940 kb on - strand, within RR42_RS00610 at 160.940 kb on - strand, within RR42_RS00610 at 160.940 kb on - strand, within RR42_RS00610 at 160.940 kb on - strand, within RR42_RS00610 at 161.011 kb on + strand, within RR42_RS00610 at 161.195 kb on - strand at 161.422 kb on - strand, within RR42_RS00615 at 161.505 kb on - strand, within RR42_RS00615 at 161.772 kb on + strand, within RR42_RS00615 at 161.772 kb on + strand, within RR42_RS00615 at 161.820 kb on + strand, within RR42_RS00615 at 161.839 kb on - strand, within RR42_RS00615 at 162.051 kb on - strand, within RR42_RS00615 at 162.219 kb on + strand, within RR42_RS00615 at 162.219 kb on + strand, within RR42_RS00615 at 162.219 kb on + strand, within RR42_RS00615 at 162.219 kb on + strand, within RR42_RS00615 at 162.220 kb on - strand, within RR42_RS00615 at 162.220 kb on - strand, within RR42_RS00615 at 162.255 kb on + strand, within RR42_RS00615 at 162.255 kb on + strand, within RR42_RS00615 at 162.479 kb on + strand at 162.479 kb on + strand at 162.479 kb on + strand at 162.480 kb on - strand at 162.480 kb on - strand at 162.519 kb on + strand, within RR42_RS00620 at 162.519 kb on + strand, within RR42_RS00620 at 162.538 kb on + strand, within RR42_RS00620 at 162.538 kb on + strand, within RR42_RS00620 at 162.538 kb on + strand, within RR42_RS00620 at 162.538 kb on + strand, within RR42_RS00620 at 162.545 kb on + strand, within RR42_RS00620 at 162.545 kb on + strand, within RR42_RS00620 at 162.545 kb on + strand, within RR42_RS00620 at 162.545 kb on + strand, within RR42_RS00620 at 162.546 kb on - strand, within RR42_RS00620 at 162.546 kb on - strand, within RR42_RS00620 at 162.546 kb on - strand, within RR42_RS00620 at 162.546 kb on - strand, within RR42_RS00620 at 162.588 kb on + strand, within RR42_RS00620 at 162.588 kb on + strand, within RR42_RS00620 at 162.588 kb on + strand, within RR42_RS00620 at 162.849 kb on + strand, within RR42_RS00620 at 162.849 kb on + strand, within RR42_RS00620 at 162.849 kb on + strand, within RR42_RS00620 at 162.849 kb on + strand, within RR42_RS00620 at 162.850 kb on - strand, within RR42_RS00620 at 162.850 kb on - strand, within RR42_RS00620 at 162.850 kb on - strand, within RR42_RS00620 at 162.850 kb on - strand, within RR42_RS00620 at 162.850 kb on - strand, within RR42_RS00620
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.00075 mM remove 160,410 + -0.4 160,420 - +0.4 160,457 + -0.3 160,458 - -0.5 160,550 - +0.3 160,606 + -0.1 160,606 + -1.0 160,607 - -0.5 160,861 + +3.0 160,862 - RR42_RS00610 0.42 -1.0 160,939 + RR42_RS00610 0.53 -0.2 160,939 + RR42_RS00610 0.53 +0.0 160,940 - RR42_RS00610 0.54 -0.7 160,940 - RR42_RS00610 0.54 -0.6 160,940 - RR42_RS00610 0.54 -0.7 160,940 - RR42_RS00610 0.54 -0.6 161,011 + RR42_RS00610 0.64 -0.9 161,195 - -0.5 161,422 - RR42_RS00615 0.12 -1.4 161,505 - RR42_RS00615 0.19 -1.1 161,772 + RR42_RS00615 0.43 -0.3 161,772 + RR42_RS00615 0.43 -0.3 161,820 + RR42_RS00615 0.47 -2.5 161,839 - RR42_RS00615 0.49 +0.1 162,051 - RR42_RS00615 0.68 -0.5 162,219 + RR42_RS00615 0.83 -0.7 162,219 + RR42_RS00615 0.83 -1.4 162,219 + RR42_RS00615 0.83 -1.2 162,219 + RR42_RS00615 0.83 -1.6 162,220 - RR42_RS00615 0.83 -0.0 162,220 - RR42_RS00615 0.83 -0.0 162,255 + RR42_RS00615 0.86 +0.0 162,255 + RR42_RS00615 0.86 -0.3 162,479 + -0.6 162,479 + -0.5 162,479 + -1.8 162,480 - +0.5 162,480 - +0.8 162,519 + RR42_RS00620 0.12 -1.3 162,519 + RR42_RS00620 0.12 +0.0 162,538 + RR42_RS00620 0.14 +0.1 162,538 + RR42_RS00620 0.14 +0.1 162,538 + RR42_RS00620 0.14 -1.1 162,538 + RR42_RS00620 0.14 -1.3 162,545 + RR42_RS00620 0.14 -0.9 162,545 + RR42_RS00620 0.14 -3.0 162,545 + RR42_RS00620 0.14 -0.8 162,545 + RR42_RS00620 0.14 -0.0 162,546 - RR42_RS00620 0.14 -0.8 162,546 - RR42_RS00620 0.14 +0.7 162,546 - RR42_RS00620 0.14 -0.7 162,546 - RR42_RS00620 0.14 +0.7 162,588 + RR42_RS00620 0.19 +0.5 162,588 + RR42_RS00620 0.19 -0.4 162,588 + RR42_RS00620 0.19 -0.0 162,849 + RR42_RS00620 0.46 -0.9 162,849 + RR42_RS00620 0.46 -0.1 162,849 + RR42_RS00620 0.46 +0.3 162,849 + RR42_RS00620 0.46 +1.3 162,850 - RR42_RS00620 0.46 -0.2 162,850 - RR42_RS00620 0.46 -0.4 162,850 - RR42_RS00620 0.46 +0.1 162,850 - RR42_RS00620 0.46 -0.1 162,850 - RR42_RS00620 0.46 +1.1
Or see this region's nucleotide sequence