Experiment: Cytidine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4862 and Pf1N1B4_4863 are separated by 338 nucleotides Pf1N1B4_4863 and Pf1N1B4_4864 overlap by 4 nucleotides
Pf1N1B4_4862 - TPR domain protein, at 5,376,951 to 5,378,006
_4862
Pf1N1B4_4863 - Outer membrane protein OprN, at 5,378,345 to 5,379,766
_4863
Pf1N1B4_4864 - Multidrug efflux transporter MexF, at 5,379,763 to 5,382,954
_4864
Position (kb) 5378
5379
5380 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5377.888 kb on + strand at 5377.889 kb on - strand, within Pf1N1B4_4862 at 5378.174 kb on - strand at 5378.174 kb on - strand at 5378.255 kb on + strand at 5378.255 kb on + strand at 5378.256 kb on - strand at 5378.256 kb on - strand at 5378.256 kb on - strand at 5378.256 kb on - strand at 5378.256 kb on - strand at 5378.256 kb on - strand at 5378.256 kb on - strand at 5378.256 kb on - strand at 5378.256 kb on - strand at 5378.346 kb on + strand at 5378.346 kb on + strand at 5378.347 kb on - strand at 5378.347 kb on - strand at 5378.347 kb on - strand at 5378.394 kb on + strand at 5378.395 kb on - strand at 5378.395 kb on - strand at 5378.395 kb on - strand at 5378.395 kb on - strand at 5378.649 kb on + strand, within Pf1N1B4_4863 at 5378.649 kb on + strand, within Pf1N1B4_4863 at 5378.649 kb on + strand, within Pf1N1B4_4863 at 5378.650 kb on - strand at 5378.650 kb on - strand, within Pf1N1B4_4863 at 5378.650 kb on - strand, within Pf1N1B4_4863 at 5378.650 kb on - strand, within Pf1N1B4_4863 at 5378.650 kb on - strand, within Pf1N1B4_4863 at 5378.650 kb on - strand, within Pf1N1B4_4863 at 5378.650 kb on - strand, within Pf1N1B4_4863 at 5378.650 kb on - strand, within Pf1N1B4_4863 at 5378.732 kb on - strand, within Pf1N1B4_4863 at 5378.732 kb on - strand, within Pf1N1B4_4863 at 5378.732 kb on - strand, within Pf1N1B4_4863 at 5379.232 kb on - strand, within Pf1N1B4_4863 at 5379.379 kb on - strand, within Pf1N1B4_4863 at 5379.851 kb on - strand at 5379.851 kb on - strand at 5379.854 kb on + strand at 5379.854 kb on + strand at 5379.855 kb on - strand at 5379.855 kb on - strand at 5379.855 kb on - strand at 5379.855 kb on - strand at 5379.855 kb on - strand at 5379.855 kb on - strand at 5379.855 kb on - strand at 5380.110 kb on + strand, within Pf1N1B4_4864 at 5380.111 kb on - strand, within Pf1N1B4_4864 at 5380.111 kb on - strand at 5380.254 kb on - strand, within Pf1N1B4_4864 at 5380.286 kb on - strand, within Pf1N1B4_4864 at 5380.337 kb on + strand at 5380.338 kb on - strand, within Pf1N1B4_4864 at 5380.476 kb on - strand, within Pf1N1B4_4864 at 5380.476 kb on - strand, within Pf1N1B4_4864 at 5380.476 kb on - strand, within Pf1N1B4_4864 at 5380.476 kb on - strand, within Pf1N1B4_4864 at 5380.625 kb on + strand, within Pf1N1B4_4864 at 5380.625 kb on + strand, within Pf1N1B4_4864 at 5380.658 kb on + strand, within Pf1N1B4_4864 at 5380.673 kb on + strand, within Pf1N1B4_4864
Per-strain Table
Position Strand Gene LocusTag Fraction Cytidine (N) remove 5,377,888 + -0.4 5,377,889 - Pf1N1B4_4862 0.89 -1.0 5,378,174 - +0.1 5,378,174 - +0.6 5,378,255 + -1.4 5,378,255 + +1.1 5,378,256 - -0.1 5,378,256 - +0.6 5,378,256 - +1.9 5,378,256 - +0.6 5,378,256 - +0.3 5,378,256 - +1.4 5,378,256 - -1.0 5,378,256 - +0.9 5,378,256 - +0.6 5,378,346 + +0.2 5,378,346 + -0.2 5,378,347 - -0.3 5,378,347 - -0.2 5,378,347 - +1.6 5,378,394 + +0.1 5,378,395 - +0.6 5,378,395 - +0.2 5,378,395 - -1.4 5,378,395 - -0.2 5,378,649 + Pf1N1B4_4863 0.21 -1.0 5,378,649 + Pf1N1B4_4863 0.21 -2.0 5,378,649 + Pf1N1B4_4863 0.21 +0.7 5,378,650 - +1.1 5,378,650 - Pf1N1B4_4863 0.21 +0.8 5,378,650 - Pf1N1B4_4863 0.21 +0.7 5,378,650 - Pf1N1B4_4863 0.21 -0.4 5,378,650 - Pf1N1B4_4863 0.21 +1.0 5,378,650 - Pf1N1B4_4863 0.21 +0.6 5,378,650 - Pf1N1B4_4863 0.21 +1.9 5,378,650 - Pf1N1B4_4863 0.21 +0.9 5,378,732 - Pf1N1B4_4863 0.27 +0.6 5,378,732 - Pf1N1B4_4863 0.27 +1.1 5,378,732 - Pf1N1B4_4863 0.27 +0.8 5,379,232 - Pf1N1B4_4863 0.62 +1.1 5,379,379 - Pf1N1B4_4863 0.73 +1.4 5,379,851 - -2.8 5,379,851 - +1.1 5,379,854 + -2.0 5,379,854 + -1.4 5,379,855 - +1.1 5,379,855 - -0.4 5,379,855 - -0.8 5,379,855 - +2.1 5,379,855 - -1.4 5,379,855 - +1.1 5,379,855 - +0.8 5,380,110 + Pf1N1B4_4864 0.11 +0.6 5,380,111 - Pf1N1B4_4864 0.11 +1.3 5,380,111 - -1.4 5,380,254 - Pf1N1B4_4864 0.15 +1.6 5,380,286 - Pf1N1B4_4864 0.16 -0.4 5,380,337 + -0.4 5,380,338 - Pf1N1B4_4864 0.18 +0.1 5,380,476 - Pf1N1B4_4864 0.22 -0.4 5,380,476 - Pf1N1B4_4864 0.22 -2.0 5,380,476 - Pf1N1B4_4864 0.22 +1.1 5,380,476 - Pf1N1B4_4864 0.22 +0.9 5,380,625 + Pf1N1B4_4864 0.27 -2.0 5,380,625 + Pf1N1B4_4864 0.27 +0.6 5,380,658 + Pf1N1B4_4864 0.28 +0.6 5,380,673 + Pf1N1B4_4864 0.29 -1.4
Or see this region's nucleotide sequence