Experiment: Cytidine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1249 and Pf1N1B4_1250 are separated by 349 nucleotides Pf1N1B4_1250 and Pf1N1B4_1251 are separated by 139 nucleotides
Pf1N1B4_1249 - Guanine deaminase (EC 3.5.4.3); Hydroxydechloroatrazine ethylaminohydrolase (EC 3.5.99.3), at 1,426,872 to 1,428,230
_1249
Pf1N1B4_1250 - Nucleoside ABC transporter, periplasmic nucleoside-binding protein, at 1,428,580 to 1,429,662
_1250
Pf1N1B4_1251 - 2-Oxobutyrate oxidase, putative, at 1,429,802 to 1,430,767
_1251
Position (kb)
1428
1429
1430 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1427.637 kb on + strand, within Pf1N1B4_1249 at 1427.637 kb on + strand, within Pf1N1B4_1249 at 1427.638 kb on - strand, within Pf1N1B4_1249 at 1427.670 kb on - strand, within Pf1N1B4_1249 at 1427.738 kb on + strand, within Pf1N1B4_1249 at 1427.739 kb on - strand, within Pf1N1B4_1249 at 1427.763 kb on - strand, within Pf1N1B4_1249 at 1427.892 kb on + strand, within Pf1N1B4_1249 at 1427.892 kb on + strand, within Pf1N1B4_1249 at 1427.892 kb on + strand, within Pf1N1B4_1249 at 1427.892 kb on + strand, within Pf1N1B4_1249 at 1427.941 kb on + strand, within Pf1N1B4_1249 at 1428.551 kb on + strand at 1428.551 kb on + strand at 1428.551 kb on + strand at 1428.552 kb on - strand at 1428.585 kb on + strand at 1428.585 kb on + strand at 1428.585 kb on + strand at 1428.585 kb on + strand at 1428.585 kb on + strand at 1428.586 kb on - strand at 1428.688 kb on + strand at 1428.688 kb on + strand at 1428.688 kb on + strand at 1428.689 kb on - strand, within Pf1N1B4_1250 at 1428.689 kb on - strand, within Pf1N1B4_1250 at 1428.721 kb on + strand, within Pf1N1B4_1250 at 1428.721 kb on + strand, within Pf1N1B4_1250 at 1428.721 kb on + strand, within Pf1N1B4_1250 at 1428.722 kb on - strand, within Pf1N1B4_1250 at 1428.722 kb on - strand, within Pf1N1B4_1250 at 1428.784 kb on + strand at 1428.784 kb on + strand, within Pf1N1B4_1250 at 1428.784 kb on + strand, within Pf1N1B4_1250 at 1428.784 kb on + strand, within Pf1N1B4_1250 at 1428.785 kb on - strand, within Pf1N1B4_1250 at 1428.785 kb on - strand, within Pf1N1B4_1250 at 1428.785 kb on - strand, within Pf1N1B4_1250 at 1428.785 kb on - strand, within Pf1N1B4_1250 at 1428.850 kb on + strand, within Pf1N1B4_1250 at 1428.851 kb on - strand, within Pf1N1B4_1250 at 1428.851 kb on - strand, within Pf1N1B4_1250 at 1428.851 kb on - strand, within Pf1N1B4_1250 at 1428.851 kb on - strand, within Pf1N1B4_1250 at 1428.851 kb on - strand, within Pf1N1B4_1250 at 1428.880 kb on + strand, within Pf1N1B4_1250 at 1428.974 kb on - strand, within Pf1N1B4_1250 at 1428.974 kb on - strand, within Pf1N1B4_1250 at 1428.974 kb on - strand, within Pf1N1B4_1250 at 1428.974 kb on - strand, within Pf1N1B4_1250 at 1428.988 kb on + strand, within Pf1N1B4_1250 at 1428.988 kb on + strand, within Pf1N1B4_1250 at 1428.988 kb on + strand, within Pf1N1B4_1250 at 1428.989 kb on - strand, within Pf1N1B4_1250 at 1428.989 kb on - strand, within Pf1N1B4_1250 at 1428.989 kb on - strand, within Pf1N1B4_1250 at 1428.989 kb on - strand, within Pf1N1B4_1250 at 1428.989 kb on - strand, within Pf1N1B4_1250 at 1429.120 kb on + strand, within Pf1N1B4_1250 at 1429.120 kb on + strand at 1429.120 kb on + strand at 1429.121 kb on - strand, within Pf1N1B4_1250 at 1429.121 kb on - strand, within Pf1N1B4_1250 at 1429.121 kb on - strand, within Pf1N1B4_1250 at 1429.121 kb on - strand, within Pf1N1B4_1250 at 1429.121 kb on - strand, within Pf1N1B4_1250 at 1429.121 kb on - strand, within Pf1N1B4_1250 at 1429.121 kb on - strand, within Pf1N1B4_1250 at 1429.282 kb on + strand, within Pf1N1B4_1250 at 1429.282 kb on + strand, within Pf1N1B4_1250 at 1429.282 kb on + strand, within Pf1N1B4_1250 at 1429.283 kb on - strand, within Pf1N1B4_1250 at 1429.366 kb on + strand, within Pf1N1B4_1250 at 1429.366 kb on + strand, within Pf1N1B4_1250 at 1429.366 kb on + strand, within Pf1N1B4_1250 at 1429.367 kb on - strand, within Pf1N1B4_1250 at 1429.367 kb on - strand, within Pf1N1B4_1250 at 1429.367 kb on - strand, within Pf1N1B4_1250 at 1429.367 kb on - strand, within Pf1N1B4_1250 at 1429.418 kb on - strand, within Pf1N1B4_1250 at 1429.482 kb on - strand, within Pf1N1B4_1250 at 1429.539 kb on + strand, within Pf1N1B4_1250 at 1429.539 kb on + strand, within Pf1N1B4_1250 at 1429.624 kb on + strand at 1429.624 kb on + strand at 1429.624 kb on + strand at 1429.624 kb on + strand at 1429.625 kb on - strand at 1429.625 kb on - strand at 1429.625 kb on - strand at 1429.625 kb on - strand at 1429.625 kb on - strand at 1429.625 kb on - strand at 1429.625 kb on - strand at 1429.625 kb on - strand at 1429.625 kb on - strand at 1429.625 kb on - strand at 1429.627 kb on + strand at 1429.627 kb on + strand at 1429.627 kb on + strand at 1429.627 kb on + strand at 1429.627 kb on + strand at 1429.627 kb on + strand at 1429.627 kb on + strand at 1429.627 kb on + strand at 1429.628 kb on - strand at 1429.628 kb on - strand at 1429.628 kb on - strand at 1429.661 kb on - strand at 1429.661 kb on - strand at 1429.838 kb on + strand at 1429.913 kb on + strand, within Pf1N1B4_1251 at 1429.913 kb on + strand, within Pf1N1B4_1251 at 1429.914 kb on - strand at 1429.914 kb on - strand, within Pf1N1B4_1251 at 1429.914 kb on - strand, within Pf1N1B4_1251 at 1429.915 kb on + strand, within Pf1N1B4_1251 at 1429.916 kb on - strand, within Pf1N1B4_1251 at 1430.219 kb on + strand, within Pf1N1B4_1251 at 1430.219 kb on + strand, within Pf1N1B4_1251 at 1430.219 kb on + strand, within Pf1N1B4_1251 at 1430.219 kb on + strand, within Pf1N1B4_1251 at 1430.219 kb on + strand, within Pf1N1B4_1251 at 1430.219 kb on + strand, within Pf1N1B4_1251 at 1430.220 kb on - strand, within Pf1N1B4_1251 at 1430.220 kb on - strand, within Pf1N1B4_1251 at 1430.220 kb on - strand, within Pf1N1B4_1251 at 1430.424 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cytidine (N) remove 1,427,637 + Pf1N1B4_1249 0.56 -1.4 1,427,637 + Pf1N1B4_1249 0.56 -0.8 1,427,638 - Pf1N1B4_1249 0.56 +0.8 1,427,670 - Pf1N1B4_1249 0.59 -0.2 1,427,738 + Pf1N1B4_1249 0.64 +0.8 1,427,739 - Pf1N1B4_1249 0.64 +1.2 1,427,763 - Pf1N1B4_1249 0.66 +2.2 1,427,892 + Pf1N1B4_1249 0.75 +0.2 1,427,892 + Pf1N1B4_1249 0.75 +0.5 1,427,892 + Pf1N1B4_1249 0.75 -1.8 1,427,892 + Pf1N1B4_1249 0.75 +1.4 1,427,941 + Pf1N1B4_1249 0.79 -0.8 1,428,551 + -0.5 1,428,551 + +0.2 1,428,551 + +0.2 1,428,552 - -2.1 1,428,585 + -2.1 1,428,585 + -1.4 1,428,585 + +0.2 1,428,585 + -0.8 1,428,585 + +0.2 1,428,586 - +1.2 1,428,688 + -0.8 1,428,688 + +0.2 1,428,688 + -0.4 1,428,689 - Pf1N1B4_1250 0.10 -0.8 1,428,689 - Pf1N1B4_1250 0.10 -0.4 1,428,721 + Pf1N1B4_1250 0.13 -0.8 1,428,721 + Pf1N1B4_1250 0.13 +2.2 1,428,721 + Pf1N1B4_1250 0.13 -1.4 1,428,722 - Pf1N1B4_1250 0.13 -0.8 1,428,722 - Pf1N1B4_1250 0.13 -0.2 1,428,784 + +0.2 1,428,784 + Pf1N1B4_1250 0.19 -0.8 1,428,784 + Pf1N1B4_1250 0.19 -1.4 1,428,784 + Pf1N1B4_1250 0.19 +0.2 1,428,785 - Pf1N1B4_1250 0.19 -1.8 1,428,785 - Pf1N1B4_1250 0.19 -0.8 1,428,785 - Pf1N1B4_1250 0.19 -0.1 1,428,785 - Pf1N1B4_1250 0.19 +0.2 1,428,850 + Pf1N1B4_1250 0.25 -1.8 1,428,851 - Pf1N1B4_1250 0.25 +0.2 1,428,851 - Pf1N1B4_1250 0.25 -2.1 1,428,851 - Pf1N1B4_1250 0.25 +1.2 1,428,851 - Pf1N1B4_1250 0.25 +0.9 1,428,851 - Pf1N1B4_1250 0.25 -1.4 1,428,880 + Pf1N1B4_1250 0.28 +0.2 1,428,974 - Pf1N1B4_1250 0.36 -0.8 1,428,974 - Pf1N1B4_1250 0.36 +0.2 1,428,974 - Pf1N1B4_1250 0.36 -0.8 1,428,974 - Pf1N1B4_1250 0.36 -1.4 1,428,988 + Pf1N1B4_1250 0.38 -2.1 1,428,988 + Pf1N1B4_1250 0.38 -0.8 1,428,988 + Pf1N1B4_1250 0.38 +0.2 1,428,989 - Pf1N1B4_1250 0.38 +0.2 1,428,989 - Pf1N1B4_1250 0.38 -0.8 1,428,989 - Pf1N1B4_1250 0.38 -0.0 1,428,989 - Pf1N1B4_1250 0.38 +0.8 1,428,989 - Pf1N1B4_1250 0.38 +0.2 1,429,120 + Pf1N1B4_1250 0.50 -0.1 1,429,120 + +0.2 1,429,120 + +0.2 1,429,121 - Pf1N1B4_1250 0.50 -2.1 1,429,121 - Pf1N1B4_1250 0.50 -0.8 1,429,121 - Pf1N1B4_1250 0.50 +1.2 1,429,121 - Pf1N1B4_1250 0.50 -1.8 1,429,121 - Pf1N1B4_1250 0.50 +0.5 1,429,121 - Pf1N1B4_1250 0.50 -1.4 1,429,121 - Pf1N1B4_1250 0.50 -0.5 1,429,282 + Pf1N1B4_1250 0.65 +0.2 1,429,282 + Pf1N1B4_1250 0.65 -0.4 1,429,282 + Pf1N1B4_1250 0.65 -2.4 1,429,283 - Pf1N1B4_1250 0.65 +0.2 1,429,366 + Pf1N1B4_1250 0.73 +0.2 1,429,366 + Pf1N1B4_1250 0.73 -0.8 1,429,366 + Pf1N1B4_1250 0.73 -2.1 1,429,367 - Pf1N1B4_1250 0.73 -1.8 1,429,367 - Pf1N1B4_1250 0.73 -0.8 1,429,367 - Pf1N1B4_1250 0.73 -0.8 1,429,367 - Pf1N1B4_1250 0.73 -0.8 1,429,418 - Pf1N1B4_1250 0.77 -1.4 1,429,482 - Pf1N1B4_1250 0.83 -0.8 1,429,539 + Pf1N1B4_1250 0.89 -1.4 1,429,539 + Pf1N1B4_1250 0.89 -1.8 1,429,624 + -0.5 1,429,624 + -1.4 1,429,624 + -1.8 1,429,624 + -0.4 1,429,625 - +1.8 1,429,625 - +1.2 1,429,625 - +1.8 1,429,625 - +0.6 1,429,625 - -2.1 1,429,625 - -2.1 1,429,625 - -1.8 1,429,625 - +1.2 1,429,625 - -1.4 1,429,625 - -0.8 1,429,627 + -2.0 1,429,627 + -1.4 1,429,627 + +0.2 1,429,627 + -0.8 1,429,627 + +0.2 1,429,627 + -0.8 1,429,627 + -0.4 1,429,627 + +1.2 1,429,628 - +0.8 1,429,628 - -2.1 1,429,628 - -1.4 1,429,661 - -0.5 1,429,661 - -0.8 1,429,838 + -0.8 1,429,913 + Pf1N1B4_1251 0.11 +1.2 1,429,913 + Pf1N1B4_1251 0.11 +0.8 1,429,914 - +1.2 1,429,914 - Pf1N1B4_1251 0.12 +1.2 1,429,914 - Pf1N1B4_1251 0.12 +0.2 1,429,915 + Pf1N1B4_1251 0.12 +1.2 1,429,916 - Pf1N1B4_1251 0.12 +0.2 1,430,219 + Pf1N1B4_1251 0.43 +0.2 1,430,219 + Pf1N1B4_1251 0.43 +1.2 1,430,219 + Pf1N1B4_1251 0.43 -2.5 1,430,219 + Pf1N1B4_1251 0.43 +0.8 1,430,219 + Pf1N1B4_1251 0.43 -0.8 1,430,219 + Pf1N1B4_1251 0.43 -0.8 1,430,220 - Pf1N1B4_1251 0.43 -1.8 1,430,220 - Pf1N1B4_1251 0.43 +0.5 1,430,220 - Pf1N1B4_1251 0.43 +2.5 1,430,424 - +0.2
Or see this region's nucleotide sequence