Experiment: Formate with hypochlorite 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08720 and MMP_RS09070 are separated by 89 nucleotides MMP_RS09070 and MMP_RS08725 are separated by 15 nucleotides MMP_RS08725 and MMP_RS08730 are separated by 41 nucleotides
MMP_RS08720: MMP_RS08720 - 4Fe-4S binding protein, at 1,632,705 to 1,633,892
_RS08720
MMP_RS09070: MMP_RS09070 - F420 non-reducing hydrogenase subunit, at 1,633,982 to 1,634,116
_RS09070
MMP_RS08725: MMP_RS08725 - Ni/Fe hydrogenase subunit alpha, at 1,634,132 to 1,635,388
_RS08725
MMP_RS08730: MMP_RS08730 - F420-non-reducing hydrogenase subunit, at 1,635,430 to 1,636,296
_RS08730
Position (kb)
1634
1635
1636 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1633.411 kb on + strand, within MMP_RS08720 at 1633.412 kb on - strand, within MMP_RS08720 at 1633.668 kb on - strand, within MMP_RS08720 at 1633.746 kb on - strand, within MMP_RS08720 at 1634.354 kb on - strand, within MMP_RS08725 at 1634.721 kb on - strand, within MMP_RS08725 at 1635.324 kb on + strand at 1635.424 kb on + strand at 1635.892 kb on + strand at 1635.999 kb on + strand at 1636.112 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate with hypochlorite 5 mM remove 1,633,411 + MMP_RS08720 0.59 -1.7 1,633,412 - MMP_RS08720 0.60 -2.4 1,633,668 - MMP_RS08720 0.81 -2.1 1,633,746 - MMP_RS08720 0.88 -2.2 1,634,354 - MMP_RS08725 0.18 -0.7 1,634,721 - MMP_RS08725 0.47 -2.6 1,635,324 + -3.1 1,635,424 + -4.2 1,635,892 + -3.1 1,635,999 + -3.5 1,636,112 + -2.9
Or see this region's nucleotide sequence