Strain Fitness in Methanococcus maripaludis S2 around MMP_RS06965

Experiment: Formate with hypochlorite 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS08915 and MMP_RS06960 are separated by 27 nucleotidesMMP_RS06960 and MMP_RS06965 are separated by 16 nucleotidesMMP_RS06965 and MMP_RS06970 are separated by 6 nucleotidesMMP_RS06970 and MMP_RS06975 are separated by 170 nucleotides MMP_RS08915: MMP_RS08915 - 2 5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate reductase, at 1,327,842 to 1,328,924 _RS08915 MMP_RS06960: MMP_RS06960 - NAD+ synthase, at 1,328,952 to 1,329,728 _RS06960 MMP_RS06965: MMP_RS06965 - TIGR04014 family B12-binding domain/radical SAM domain-containing protein, at 1,329,745 to 1,331,058 _RS06965 MMP_RS06970: MMP_RS06970 - ribosomal protein S18-alanine N-acetyltransferase, at 1,331,065 to 1,331,550 _RS06970 MMP_RS06975: MMP_RS06975 - sulfide-dependent adenosine diphosphate thiazole synthase, at 1,331,721 to 1,332,509 _RS06975 Position (kb) 1329 1330 1331 1332Strain fitness (log2 ratio) -3 -2 -1 0 1at 1329.051 kb on + strandat 1329.290 kb on + strandat 1329.347 kb on + strandat 1329.349 kb on - strandat 1329.352 kb on + strandat 1329.546 kb on - strandat 1329.735 kb on + strandat 1329.768 kb on - strandat 1329.768 kb on - strandat 1329.768 kb on - strandat 1329.796 kb on + strandat 1329.849 kb on - strandat 1329.923 kb on + strand, within MMP_RS06965at 1329.930 kb on + strand, within MMP_RS06965at 1330.037 kb on + strand, within MMP_RS06965at 1330.068 kb on - strand, within MMP_RS06965at 1330.283 kb on + strand, within MMP_RS06965at 1330.346 kb on + strand, within MMP_RS06965at 1330.348 kb on + strand, within MMP_RS06965at 1331.008 kb on - strandat 1331.008 kb on - strandat 1331.043 kb on + strandat 1331.063 kb on + strandat 1331.129 kb on + strand, within MMP_RS06970at 1331.151 kb on + strand, within MMP_RS06970at 1331.154 kb on + strand, within MMP_RS06970at 1331.154 kb on + strand, within MMP_RS06970at 1331.157 kb on - strand, within MMP_RS06970at 1331.157 kb on - strand, within MMP_RS06970at 1331.159 kb on - strand, within MMP_RS06970at 1331.286 kb on - strand, within MMP_RS06970at 1331.323 kb on - strand, within MMP_RS06970at 1331.347 kb on + strand, within MMP_RS06970at 1331.442 kb on + strand, within MMP_RS06970at 1331.442 kb on + strand, within MMP_RS06970at 1331.443 kb on - strand, within MMP_RS06970at 1331.444 kb on + strand, within MMP_RS06970at 1331.542 kb on - strandat 1331.551 kb on - strandat 1331.551 kb on - strandat 1331.553 kb on - strandat 1331.553 kb on - strandat 1331.554 kb on + strandat 1331.554 kb on + strandat 1331.554 kb on + strandat 1331.593 kb on + strandat 1331.598 kb on + strandat 1331.600 kb on + strandat 1331.666 kb on - strandat 1331.708 kb on + strandat 1331.776 kb on - strandat 1331.876 kb on - strand, within MMP_RS06975at 1332.005 kb on + strand, within MMP_RS06975at 1332.005 kb on + strand, within MMP_RS06975

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Per-strain Table

Position Strand Gene LocusTag Fraction Formate with hypochlorite 5 mM
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1,329,051 + -1.9
1,329,290 + -0.4
1,329,347 + -3.1
1,329,349 - -3.3
1,329,352 + -3.1
1,329,546 - -2.7
1,329,735 + +0.2
1,329,768 - +0.0
1,329,768 - -0.4
1,329,768 - -1.5
1,329,796 + +0.2
1,329,849 - +0.4
1,329,923 + MMP_RS06965 0.14 +0.4
1,329,930 + MMP_RS06965 0.14 +0.2
1,330,037 + MMP_RS06965 0.22 -0.3
1,330,068 - MMP_RS06965 0.25 +0.4
1,330,283 + MMP_RS06965 0.41 +0.7
1,330,346 + MMP_RS06965 0.46 +0.2
1,330,348 + MMP_RS06965 0.46 +0.2
1,331,008 - +0.2
1,331,008 - +0.5
1,331,043 + -0.2
1,331,063 + +0.9
1,331,129 + MMP_RS06970 0.13 +0.9
1,331,151 + MMP_RS06970 0.18 -0.5
1,331,154 + MMP_RS06970 0.18 +0.3
1,331,154 + MMP_RS06970 0.18 -0.1
1,331,157 - MMP_RS06970 0.19 -0.3
1,331,157 - MMP_RS06970 0.19 +0.2
1,331,159 - MMP_RS06970 0.19 +0.3
1,331,286 - MMP_RS06970 0.45 +0.4
1,331,323 - MMP_RS06970 0.53 +0.2
1,331,347 + MMP_RS06970 0.58 -0.8
1,331,442 + MMP_RS06970 0.78 -1.2
1,331,442 + MMP_RS06970 0.78 -0.5
1,331,443 - MMP_RS06970 0.78 +0.4
1,331,444 + MMP_RS06970 0.78 +1.1
1,331,542 - -0.5
1,331,551 - +0.6
1,331,551 - +0.4
1,331,553 - +0.2
1,331,553 - +0.1
1,331,554 + +0.2
1,331,554 + +0.9
1,331,554 + -1.6
1,331,593 + -1.0
1,331,598 + +0.3
1,331,600 + +0.0
1,331,666 - +0.5
1,331,708 + +0.5
1,331,776 - -1.0
1,331,876 - MMP_RS06975 0.20 -0.4
1,332,005 + MMP_RS06975 0.36 +0.7
1,332,005 + MMP_RS06975 0.36 -0.5

Or see this region's nucleotide sequence