Experiment: Survival; Treatment=UGA synthetic gw pH7.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_18965 and IAI47_18970 are separated by 185 nucleotides IAI47_18970 and IAI47_18975 are separated by 115 nucleotides IAI47_18975 and IAI47_18980 are separated by 43 nucleotides IAI47_18980 and IAI47_18985 overlap by 4 nucleotides
IAI47_18965: IAI47_18965 - DUF1173 family protein, at 26,379 to 27,596
_18965
IAI47_18970: IAI47_18970 - hypothetical protein, at 27,782 to 28,102
_18970
IAI47_18975: IAI47_18975 - B3/4 domain-containing protein, at 28,218 to 28,913
_18975
IAI47_18980: IAI47_18980 - helix-turn-helix domain-containing protein, at 28,957 to 29,559
_18980
IAI47_18985: IAI47_18985 - MarR family transcriptional regulator, at 29,556 to 30,071
_18985
Position (kb) 28
29 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 27.255 kb on - strand, within IAI47_18965 at 27.279 kb on + strand, within IAI47_18965 at 27.349 kb on - strand, within IAI47_18965 at 27.349 kb on - strand, within IAI47_18965 at 27.349 kb on - strand, within IAI47_18965 at 27.355 kb on + strand, within IAI47_18965 at 27.355 kb on + strand, within IAI47_18965 at 27.355 kb on + strand, within IAI47_18965 at 27.355 kb on + strand, within IAI47_18965 at 27.355 kb on + strand, within IAI47_18965 at 27.356 kb on - strand, within IAI47_18965 at 27.356 kb on - strand, within IAI47_18965 at 27.365 kb on + strand, within IAI47_18965 at 27.365 kb on + strand, within IAI47_18965 at 27.366 kb on - strand, within IAI47_18965 at 27.366 kb on - strand, within IAI47_18965 at 27.366 kb on - strand, within IAI47_18965 at 27.370 kb on + strand, within IAI47_18965 at 27.370 kb on + strand, within IAI47_18965 at 27.370 kb on + strand, within IAI47_18965 at 27.380 kb on - strand, within IAI47_18965 at 27.380 kb on - strand, within IAI47_18965 at 27.421 kb on + strand, within IAI47_18965 at 27.421 kb on + strand, within IAI47_18965 at 27.422 kb on - strand, within IAI47_18965 at 27.449 kb on + strand, within IAI47_18965 at 27.450 kb on - strand, within IAI47_18965 at 27.504 kb on + strand at 27.505 kb on - strand at 27.516 kb on + strand at 27.524 kb on + strand at 27.524 kb on + strand at 27.528 kb on + strand at 27.528 kb on + strand at 27.529 kb on - strand at 27.529 kb on - strand at 27.549 kb on + strand at 27.549 kb on + strand at 27.550 kb on - strand at 27.553 kb on - strand at 27.553 kb on - strand at 27.553 kb on - strand at 27.553 kb on - strand at 27.572 kb on + strand at 27.596 kb on + strand at 27.597 kb on - strand at 27.597 kb on - strand at 27.623 kb on + strand at 27.630 kb on + strand at 27.631 kb on - strand at 27.648 kb on + strand at 27.648 kb on + strand at 27.648 kb on + strand at 27.648 kb on + strand at 27.648 kb on + strand at 27.649 kb on - strand at 27.649 kb on - strand at 27.649 kb on - strand at 27.649 kb on - strand at 27.649 kb on - strand at 27.666 kb on + strand at 27.666 kb on + strand at 27.666 kb on + strand at 27.667 kb on - strand at 27.667 kb on - strand at 27.667 kb on - strand at 27.671 kb on - strand at 27.704 kb on + strand at 27.705 kb on - strand at 27.705 kb on - strand at 27.726 kb on + strand at 27.726 kb on + strand at 27.727 kb on - strand at 27.727 kb on - strand at 27.727 kb on - strand at 27.727 kb on - strand at 27.729 kb on + strand at 27.729 kb on + strand at 27.729 kb on + strand at 27.730 kb on - strand at 27.730 kb on - strand at 27.747 kb on - strand at 27.758 kb on + strand at 27.763 kb on + strand at 27.763 kb on + strand at 27.763 kb on + strand at 27.764 kb on - strand at 27.764 kb on - strand at 27.764 kb on - strand at 27.764 kb on - strand at 27.764 kb on - strand at 27.814 kb on + strand, within IAI47_18970 at 27.815 kb on - strand, within IAI47_18970 at 27.892 kb on + strand, within IAI47_18970 at 27.892 kb on + strand, within IAI47_18970 at 27.892 kb on + strand, within IAI47_18970 at 27.893 kb on - strand, within IAI47_18970 at 27.893 kb on - strand, within IAI47_18970 at 27.893 kb on - strand, within IAI47_18970 at 27.893 kb on - strand, within IAI47_18970 at 27.893 kb on - strand, within IAI47_18970 at 27.893 kb on - strand, within IAI47_18970 at 27.894 kb on + strand, within IAI47_18970 at 27.894 kb on + strand, within IAI47_18970 at 27.894 kb on + strand, within IAI47_18970 at 27.894 kb on + strand, within IAI47_18970 at 27.895 kb on - strand, within IAI47_18970 at 27.895 kb on - strand, within IAI47_18970 at 27.895 kb on - strand, within IAI47_18970 at 27.895 kb on - strand, within IAI47_18970 at 27.895 kb on - strand, within IAI47_18970 at 27.895 kb on - strand, within IAI47_18970 at 27.895 kb on - strand, within IAI47_18970 at 27.895 kb on - strand, within IAI47_18970 at 27.916 kb on - strand, within IAI47_18970 at 27.935 kb on + strand, within IAI47_18970 at 27.935 kb on + strand, within IAI47_18970 at 27.936 kb on - strand, within IAI47_18970 at 27.936 kb on - strand, within IAI47_18970 at 27.936 kb on - strand, within IAI47_18970 at 27.936 kb on - strand, within IAI47_18970 at 28.005 kb on + strand, within IAI47_18970 at 28.005 kb on + strand, within IAI47_18970 at 28.005 kb on + strand, within IAI47_18970 at 28.006 kb on - strand, within IAI47_18970 at 28.008 kb on + strand, within IAI47_18970 at 28.008 kb on + strand, within IAI47_18970 at 28.008 kb on + strand, within IAI47_18970 at 28.009 kb on - strand, within IAI47_18970 at 28.009 kb on - strand, within IAI47_18970 at 28.009 kb on - strand, within IAI47_18970 at 28.009 kb on - strand, within IAI47_18970 at 28.084 kb on + strand at 28.085 kb on - strand at 28.086 kb on + strand at 28.086 kb on + strand at 28.086 kb on + strand at 28.087 kb on - strand at 28.087 kb on - strand at 28.097 kb on - strand at 28.103 kb on + strand at 28.103 kb on + strand at 28.103 kb on + strand at 28.103 kb on + strand at 28.103 kb on + strand at 28.104 kb on - strand at 28.104 kb on - strand at 28.104 kb on - strand at 28.104 kb on - strand at 28.104 kb on - strand at 28.105 kb on + strand at 28.105 kb on + strand at 28.105 kb on + strand at 28.105 kb on + strand at 28.106 kb on - strand at 28.106 kb on - strand at 28.106 kb on - strand at 28.118 kb on + strand at 28.118 kb on + strand at 28.118 kb on + strand at 28.118 kb on + strand at 28.118 kb on + strand at 28.118 kb on + strand at 28.118 kb on + strand at 28.119 kb on - strand at 28.119 kb on - strand at 28.119 kb on - strand at 28.119 kb on - strand at 28.119 kb on - strand at 28.187 kb on + strand at 28.278 kb on + strand at 28.279 kb on - strand at 28.279 kb on - strand at 28.329 kb on - strand, within IAI47_18975 at 28.349 kb on + strand, within IAI47_18975 at 28.350 kb on - strand, within IAI47_18975 at 28.423 kb on - strand, within IAI47_18975 at 28.545 kb on - strand, within IAI47_18975 at 28.546 kb on + strand, within IAI47_18975 at 28.546 kb on + strand, within IAI47_18975 at 28.546 kb on + strand, within IAI47_18975 at 28.546 kb on + strand, within IAI47_18975 at 28.546 kb on + strand, within IAI47_18975 at 28.546 kb on + strand, within IAI47_18975 at 28.546 kb on + strand, within IAI47_18975 at 28.547 kb on - strand, within IAI47_18975 at 28.547 kb on - strand, within IAI47_18975 at 28.547 kb on - strand, within IAI47_18975 at 28.547 kb on - strand, within IAI47_18975 at 28.583 kb on - strand, within IAI47_18975 at 28.603 kb on + strand, within IAI47_18975 at 28.603 kb on + strand, within IAI47_18975 at 28.603 kb on + strand, within IAI47_18975 at 28.603 kb on + strand, within IAI47_18975 at 28.698 kb on - strand, within IAI47_18975 at 28.822 kb on - strand, within IAI47_18975 at 28.830 kb on - strand, within IAI47_18975 at 28.830 kb on - strand, within IAI47_18975 at 28.836 kb on + strand, within IAI47_18975 at 28.836 kb on + strand, within IAI47_18975 at 28.908 kb on + strand at 28.908 kb on + strand at 28.908 kb on + strand at 28.908 kb on + strand at 28.908 kb on + strand at 28.909 kb on - strand at 28.909 kb on - strand at 28.909 kb on - strand at 28.909 kb on - strand at 28.933 kb on + strand at 28.939 kb on - strand at 28.946 kb on - strand at 28.948 kb on + strand at 28.977 kb on - strand at 29.010 kb on + strand at 29.010 kb on + strand at 29.046 kb on + strand, within IAI47_18980 at 29.046 kb on + strand, within IAI47_18980 at 29.047 kb on - strand, within IAI47_18980 at 29.098 kb on - strand, within IAI47_18980 at 29.313 kb on + strand, within IAI47_18980 at 29.325 kb on - strand, within IAI47_18980 at 29.325 kb on - strand, within IAI47_18980 at 29.484 kb on + strand, within IAI47_18980 at 29.484 kb on + strand, within IAI47_18980 at 29.484 kb on + strand, within IAI47_18980 at 29.484 kb on + strand, within IAI47_18980 at 29.485 kb on - strand, within IAI47_18980 at 29.485 kb on - strand, within IAI47_18980 at 29.497 kb on + strand, within IAI47_18980 at 29.497 kb on + strand, within IAI47_18980 at 29.497 kb on + strand, within IAI47_18980 at 29.498 kb on - strand, within IAI47_18980 at 29.498 kb on - strand, within IAI47_18980 at 29.498 kb on - strand, within IAI47_18980 at 29.498 kb on - strand, within IAI47_18980 at 29.523 kb on + strand at 29.579 kb on + strand at 29.580 kb on - strand at 29.580 kb on - strand at 29.580 kb on - strand at 29.580 kb on - strand at 29.750 kb on + strand, within IAI47_18985 at 29.750 kb on + strand, within IAI47_18985 at 29.751 kb on - strand, within IAI47_18985 at 29.751 kb on - strand, within IAI47_18985 at 29.751 kb on - strand, within IAI47_18985 at 29.751 kb on - strand, within IAI47_18985 at 29.751 kb on - strand, within IAI47_18985 at 29.830 kb on - strand, within IAI47_18985 at 29.845 kb on + strand, within IAI47_18985 at 29.901 kb on + strand, within IAI47_18985 at 29.901 kb on + strand, within IAI47_18985 at 29.902 kb on - strand, within IAI47_18985 at 29.902 kb on - strand, within IAI47_18985 at 29.902 kb on - strand, within IAI47_18985 at 29.902 kb on - strand, within IAI47_18985 at 29.902 kb on - strand, within IAI47_18985 at 29.902 kb on - strand, within IAI47_18985
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 27,255 - IAI47_18965 0.72 +0.5 27,279 + IAI47_18965 0.74 -1.0 27,349 - IAI47_18965 0.80 -1.3 27,349 - IAI47_18965 0.80 -3.0 27,349 - IAI47_18965 0.80 -0.8 27,355 + IAI47_18965 0.80 +1.9 27,355 + IAI47_18965 0.80 -0.3 27,355 + IAI47_18965 0.80 +0.6 27,355 + IAI47_18965 0.80 +0.3 27,355 + IAI47_18965 0.80 +2.9 27,356 - IAI47_18965 0.80 -0.9 27,356 - IAI47_18965 0.80 -0.2 27,365 + IAI47_18965 0.81 +0.0 27,365 + IAI47_18965 0.81 -1.3 27,366 - IAI47_18965 0.81 -0.3 27,366 - IAI47_18965 0.81 -0.3 27,366 - IAI47_18965 0.81 -1.6 27,370 + IAI47_18965 0.81 -0.3 27,370 + IAI47_18965 0.81 +0.6 27,370 + IAI47_18965 0.81 -0.7 27,380 - IAI47_18965 0.82 +2.7 27,380 - IAI47_18965 0.82 +2.7 27,421 + IAI47_18965 0.86 -0.7 27,421 + IAI47_18965 0.86 -2.8 27,422 - IAI47_18965 0.86 -0.4 27,449 + IAI47_18965 0.88 +0.7 27,450 - IAI47_18965 0.88 -2.3 27,504 + -3.1 27,505 - -1.9 27,516 + -1.0 27,524 + -1.3 27,524 + -2.5 27,528 + -0.4 27,528 + +0.9 27,529 - +0.3 27,529 - +0.5 27,549 + +0.7 27,549 + +1.2 27,550 - +2.4 27,553 - -0.5 27,553 - -0.3 27,553 - -0.7 27,553 - -1.5 27,572 + -3.4 27,596 + -1.2 27,597 - +0.5 27,597 - +0.3 27,623 + +1.3 27,630 + +1.0 27,631 - +5.4 27,648 + -2.5 27,648 + -0.4 27,648 + +0.6 27,648 + -0.9 27,648 + -2.1 27,649 - +0.6 27,649 - -0.3 27,649 - +1.3 27,649 - +0.5 27,649 - -1.0 27,666 + -2.3 27,666 + +2.3 27,666 + +2.5 27,667 - -2.3 27,667 - +0.1 27,667 - -1.5 27,671 - -0.7 27,704 + -1.9 27,705 - -2.1 27,705 - +0.0 27,726 + -0.1 27,726 + -0.9 27,727 - +0.5 27,727 - +1.3 27,727 - +1.3 27,727 - +1.3 27,729 + +0.7 27,729 + +0.5 27,729 + +0.3 27,730 - +2.3 27,730 - +0.5 27,747 - +0.3 27,758 + -1.9 27,763 + +0.6 27,763 + +0.1 27,763 + +1.8 27,764 - -0.2 27,764 - -0.3 27,764 - -1.0 27,764 - +2.0 27,764 - +1.4 27,814 + IAI47_18970 0.10 -1.6 27,815 - IAI47_18970 0.10 +2.1 27,892 + IAI47_18970 0.34 -0.7 27,892 + IAI47_18970 0.34 +5.3 27,892 + IAI47_18970 0.34 +1.3 27,893 - IAI47_18970 0.35 +1.0 27,893 - IAI47_18970 0.35 -0.8 27,893 - IAI47_18970 0.35 -0.2 27,893 - IAI47_18970 0.35 +0.7 27,893 - IAI47_18970 0.35 -2.2 27,893 - IAI47_18970 0.35 -3.2 27,894 + IAI47_18970 0.35 -2.3 27,894 + IAI47_18970 0.35 +2.6 27,894 + IAI47_18970 0.35 -0.2 27,894 + IAI47_18970 0.35 -0.7 27,895 - IAI47_18970 0.35 -3.8 27,895 - IAI47_18970 0.35 +2.0 27,895 - IAI47_18970 0.35 +0.7 27,895 - IAI47_18970 0.35 +0.4 27,895 - IAI47_18970 0.35 +0.5 27,895 - IAI47_18970 0.35 -1.1 27,895 - IAI47_18970 0.35 +1.0 27,895 - IAI47_18970 0.35 -2.5 27,916 - IAI47_18970 0.42 +3.3 27,935 + IAI47_18970 0.48 +1.6 27,935 + IAI47_18970 0.48 -1.7 27,936 - IAI47_18970 0.48 +0.1 27,936 - IAI47_18970 0.48 +0.8 27,936 - IAI47_18970 0.48 +1.6 27,936 - IAI47_18970 0.48 -0.6 28,005 + IAI47_18970 0.69 +1.7 28,005 + IAI47_18970 0.69 +1.3 28,005 + IAI47_18970 0.69 -0.2 28,006 - IAI47_18970 0.70 -0.2 28,008 + IAI47_18970 0.70 +2.0 28,008 + IAI47_18970 0.70 -2.5 28,008 + IAI47_18970 0.70 +2.1 28,009 - IAI47_18970 0.71 -1.2 28,009 - IAI47_18970 0.71 -0.9 28,009 - IAI47_18970 0.71 -0.1 28,009 - IAI47_18970 0.71 +2.3 28,084 + +0.3 28,085 - -0.5 28,086 + -0.7 28,086 + -0.9 28,086 + -1.1 28,087 - +0.9 28,087 - +0.9 28,097 - +0.3 28,103 + -0.7 28,103 + -2.8 28,103 + +0.8 28,103 + +2.0 28,103 + +1.3 28,104 - +2.6 28,104 - +1.6 28,104 - +0.3 28,104 - +0.0 28,104 - +1.1 28,105 + -0.9 28,105 + -0.8 28,105 + -0.4 28,105 + +2.3 28,106 - -1.4 28,106 - -2.4 28,106 - -0.1 28,118 + -2.0 28,118 + -1.5 28,118 + -0.3 28,118 + -3.5 28,118 + +0.8 28,118 + -0.9 28,118 + +2.4 28,119 - -0.3 28,119 - +0.3 28,119 - -1.1 28,119 - +0.3 28,119 - -0.9 28,187 + -1.9 28,278 + +1.7 28,279 - -2.5 28,279 - -2.3 28,329 - IAI47_18975 0.16 -1.7 28,349 + IAI47_18975 0.19 +1.3 28,350 - IAI47_18975 0.19 +1.1 28,423 - IAI47_18975 0.29 +0.4 28,545 - IAI47_18975 0.47 +0.8 28,546 + IAI47_18975 0.47 +1.6 28,546 + IAI47_18975 0.47 +0.4 28,546 + IAI47_18975 0.47 -1.7 28,546 + IAI47_18975 0.47 +0.6 28,546 + IAI47_18975 0.47 -1.7 28,546 + IAI47_18975 0.47 -1.8 28,546 + IAI47_18975 0.47 -4.2 28,547 - IAI47_18975 0.47 +1.3 28,547 - IAI47_18975 0.47 +2.7 28,547 - IAI47_18975 0.47 -2.9 28,547 - IAI47_18975 0.47 +0.5 28,583 - IAI47_18975 0.52 +2.0 28,603 + IAI47_18975 0.55 -1.5 28,603 + IAI47_18975 0.55 +1.1 28,603 + IAI47_18975 0.55 -0.3 28,603 + IAI47_18975 0.55 -0.2 28,698 - IAI47_18975 0.69 -0.3 28,822 - IAI47_18975 0.87 -1.4 28,830 - IAI47_18975 0.88 -0.2 28,830 - IAI47_18975 0.88 +1.3 28,836 + IAI47_18975 0.89 +1.5 28,836 + IAI47_18975 0.89 -0.7 28,908 + -1.1 28,908 + -0.1 28,908 + +2.3 28,908 + -3.3 28,908 + -0.6 28,909 - +0.6 28,909 - -1.5 28,909 - +0.4 28,909 - +1.3 28,933 + +0.6 28,939 - +0.6 28,946 - +1.3 28,948 + +1.3 28,977 - -1.0 29,010 + -0.9 29,010 + -0.3 29,046 + IAI47_18980 0.15 -2.5 29,046 + IAI47_18980 0.15 +0.3 29,047 - IAI47_18980 0.15 -1.3 29,098 - IAI47_18980 0.23 -0.4 29,313 + IAI47_18980 0.59 +1.3 29,325 - IAI47_18980 0.61 +0.6 29,325 - IAI47_18980 0.61 -0.3 29,484 + IAI47_18980 0.87 +1.8 29,484 + IAI47_18980 0.87 +2.2 29,484 + IAI47_18980 0.87 +1.9 29,484 + IAI47_18980 0.87 +1.0 29,485 - IAI47_18980 0.88 +1.0 29,485 - IAI47_18980 0.88 +0.9 29,497 + IAI47_18980 0.90 +1.1 29,497 + IAI47_18980 0.90 +1.3 29,497 + IAI47_18980 0.90 +0.3 29,498 - IAI47_18980 0.90 +3.0 29,498 - IAI47_18980 0.90 +1.3 29,498 - IAI47_18980 0.90 -1.9 29,498 - IAI47_18980 0.90 -2.2 29,523 + +1.0 29,579 + -1.3 29,580 - +1.6 29,580 - -0.7 29,580 - +1.3 29,580 - +1.5 29,750 + IAI47_18985 0.38 +1.1 29,750 + IAI47_18985 0.38 -0.3 29,751 - IAI47_18985 0.38 -2.8 29,751 - IAI47_18985 0.38 +1.3 29,751 - IAI47_18985 0.38 +1.5 29,751 - IAI47_18985 0.38 -0.3 29,751 - IAI47_18985 0.38 -1.4 29,830 - IAI47_18985 0.53 -2.2 29,845 + IAI47_18985 0.56 -2.6 29,901 + IAI47_18985 0.67 +1.3 29,901 + IAI47_18985 0.67 -1.0 29,902 - IAI47_18985 0.67 -0.3 29,902 - IAI47_18985 0.67 +0.3 29,902 - IAI47_18985 0.67 -2.0 29,902 - IAI47_18985 0.67 +1.4 29,902 - IAI47_18985 0.67 -1.3 29,902 - IAI47_18985 0.67 -0.5
Or see this region's nucleotide sequence