Experiment: Survival; Treatment=UGA synthetic gw pH7.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_02580 and IAI47_02585 overlap by 8 nucleotides IAI47_02585 and IAI47_02590 are separated by 103 nucleotides IAI47_02590 and IAI47_02595 are separated by 71 nucleotides
IAI47_02580: IAI47_02580 - DNA mismatch repair endonuclease MutL, at 533,417 to 535,282
_02580
IAI47_02585: IAI47_02585 - tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA, at 535,275 to 536,225
_02585
IAI47_02590: IAI47_02590 - RNA chaperone Hfq, at 536,329 to 536,643
_02590
IAI47_02595: IAI47_02595 - GTPase HflX, at 536,715 to 537,995
_02595
Position (kb) 535
536
537 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 534.275 kb on + strand, within IAI47_02580 at 534.275 kb on + strand, within IAI47_02580 at 534.275 kb on + strand, within IAI47_02580 at 534.276 kb on - strand, within IAI47_02580 at 534.285 kb on - strand, within IAI47_02580 at 534.285 kb on - strand, within IAI47_02580 at 534.342 kb on + strand, within IAI47_02580 at 534.343 kb on - strand, within IAI47_02580 at 534.343 kb on - strand, within IAI47_02580 at 534.343 kb on - strand, within IAI47_02580 at 534.366 kb on + strand, within IAI47_02580 at 534.366 kb on + strand, within IAI47_02580 at 534.366 kb on + strand, within IAI47_02580 at 534.366 kb on + strand, within IAI47_02580 at 534.366 kb on + strand, within IAI47_02580 at 534.367 kb on - strand, within IAI47_02580 at 534.367 kb on - strand, within IAI47_02580 at 534.367 kb on - strand, within IAI47_02580 at 534.367 kb on - strand, within IAI47_02580 at 534.367 kb on - strand, within IAI47_02580 at 534.376 kb on + strand, within IAI47_02580 at 534.376 kb on + strand, within IAI47_02580 at 534.376 kb on + strand, within IAI47_02580 at 534.376 kb on + strand, within IAI47_02580 at 534.376 kb on + strand, within IAI47_02580 at 534.377 kb on - strand, within IAI47_02580 at 534.377 kb on - strand, within IAI47_02580 at 534.377 kb on - strand, within IAI47_02580 at 534.381 kb on - strand, within IAI47_02580 at 534.381 kb on - strand, within IAI47_02580 at 534.381 kb on - strand, within IAI47_02580 at 534.588 kb on - strand, within IAI47_02580 at 534.611 kb on + strand, within IAI47_02580 at 534.798 kb on + strand, within IAI47_02580 at 534.798 kb on + strand, within IAI47_02580 at 534.798 kb on + strand, within IAI47_02580 at 534.798 kb on + strand, within IAI47_02580 at 534.798 kb on + strand, within IAI47_02580 at 534.799 kb on - strand, within IAI47_02580 at 534.799 kb on - strand, within IAI47_02580 at 534.799 kb on - strand, within IAI47_02580 at 534.799 kb on - strand, within IAI47_02580 at 534.799 kb on - strand, within IAI47_02580 at 534.799 kb on - strand, within IAI47_02580 at 534.871 kb on + strand, within IAI47_02580 at 534.871 kb on + strand, within IAI47_02580 at 534.871 kb on + strand, within IAI47_02580 at 534.872 kb on - strand, within IAI47_02580 at 534.872 kb on - strand, within IAI47_02580 at 534.872 kb on - strand, within IAI47_02580 at 534.946 kb on + strand, within IAI47_02580 at 534.946 kb on + strand, within IAI47_02580 at 534.946 kb on + strand, within IAI47_02580 at 534.947 kb on - strand, within IAI47_02580 at 535.062 kb on + strand, within IAI47_02580 at 535.062 kb on + strand, within IAI47_02580 at 535.062 kb on + strand, within IAI47_02580 at 535.062 kb on + strand, within IAI47_02580 at 535.062 kb on + strand, within IAI47_02580 at 535.062 kb on + strand, within IAI47_02580 at 535.062 kb on + strand, within IAI47_02580 at 535.063 kb on - strand, within IAI47_02580 at 535.063 kb on - strand, within IAI47_02580 at 535.063 kb on - strand, within IAI47_02580 at 535.063 kb on - strand, within IAI47_02580 at 535.067 kb on + strand, within IAI47_02580 at 535.068 kb on - strand, within IAI47_02580 at 535.139 kb on + strand at 535.139 kb on + strand at 535.230 kb on + strand at 535.230 kb on + strand at 535.230 kb on + strand at 535.230 kb on + strand at 535.231 kb on - strand at 535.231 kb on - strand at 535.231 kb on - strand at 535.231 kb on - strand at 535.231 kb on - strand at 535.231 kb on - strand at 535.231 kb on - strand at 535.233 kb on + strand at 535.233 kb on + strand at 535.233 kb on + strand at 535.233 kb on + strand at 535.233 kb on + strand at 535.234 kb on - strand at 535.234 kb on - strand at 535.234 kb on - strand at 535.242 kb on + strand at 535.242 kb on + strand at 535.285 kb on + strand at 535.286 kb on - strand at 535.368 kb on - strand at 535.376 kb on - strand, within IAI47_02585 at 535.410 kb on - strand, within IAI47_02585 at 535.413 kb on + strand, within IAI47_02585 at 535.459 kb on + strand, within IAI47_02585 at 535.460 kb on - strand, within IAI47_02585 at 535.605 kb on + strand, within IAI47_02585 at 535.606 kb on - strand, within IAI47_02585 at 535.857 kb on + strand, within IAI47_02585 at 536.043 kb on + strand, within IAI47_02585 at 536.043 kb on + strand, within IAI47_02585 at 536.044 kb on - strand, within IAI47_02585 at 536.122 kb on + strand, within IAI47_02585 at 536.122 kb on + strand, within IAI47_02585 at 536.123 kb on - strand, within IAI47_02585 at 536.189 kb on + strand at 536.190 kb on - strand at 536.190 kb on - strand at 536.207 kb on + strand at 536.207 kb on + strand at 536.208 kb on - strand at 536.231 kb on + strand at 536.269 kb on + strand at 536.283 kb on + strand at 536.283 kb on + strand at 536.283 kb on + strand at 536.283 kb on + strand at 536.283 kb on + strand at 536.283 kb on + strand at 536.283 kb on + strand at 536.283 kb on + strand at 536.283 kb on + strand at 536.283 kb on + strand at 536.284 kb on - strand at 536.290 kb on + strand at 536.290 kb on + strand at 536.290 kb on + strand at 536.290 kb on + strand at 536.290 kb on + strand at 536.290 kb on + strand at 536.290 kb on + strand at 536.290 kb on + strand at 536.290 kb on + strand at 536.290 kb on + strand at 536.293 kb on + strand at 536.293 kb on + strand at 536.312 kb on + strand at 536.312 kb on + strand at 536.312 kb on + strand at 536.312 kb on + strand at 536.313 kb on - strand at 536.325 kb on + strand at 536.348 kb on + strand at 536.415 kb on + strand, within IAI47_02590 at 536.418 kb on + strand, within IAI47_02590 at 536.477 kb on + strand, within IAI47_02590 at 536.491 kb on + strand, within IAI47_02590 at 536.491 kb on + strand, within IAI47_02590 at 536.491 kb on + strand, within IAI47_02590 at 536.491 kb on + strand, within IAI47_02590 at 536.493 kb on + strand, within IAI47_02590 at 536.494 kb on - strand, within IAI47_02590 at 536.541 kb on + strand, within IAI47_02590 at 536.547 kb on + strand, within IAI47_02590 at 536.547 kb on + strand, within IAI47_02590 at 536.547 kb on + strand, within IAI47_02590 at 536.547 kb on + strand, within IAI47_02590 at 536.547 kb on + strand, within IAI47_02590 at 536.547 kb on + strand, within IAI47_02590 at 536.547 kb on + strand, within IAI47_02590 at 536.547 kb on + strand, within IAI47_02590 at 536.548 kb on - strand, within IAI47_02590 at 536.575 kb on + strand, within IAI47_02590 at 536.575 kb on + strand, within IAI47_02590 at 536.575 kb on + strand, within IAI47_02590 at 536.575 kb on + strand, within IAI47_02590 at 536.632 kb on - strand at 536.641 kb on + strand at 536.654 kb on + strand at 536.654 kb on + strand at 536.654 kb on + strand at 536.654 kb on + strand at 536.654 kb on + strand at 536.696 kb on + strand at 536.696 kb on + strand at 536.696 kb on + strand at 536.697 kb on - strand at 536.697 kb on - strand at 536.697 kb on - strand at 536.709 kb on + strand at 536.711 kb on + strand at 536.711 kb on + strand at 536.711 kb on + strand at 536.712 kb on - strand at 536.712 kb on - strand at 536.712 kb on - strand at 536.712 kb on - strand at 536.712 kb on - strand at 536.712 kb on - strand at 536.712 kb on - strand at 536.712 kb on - strand at 536.712 kb on - strand at 536.755 kb on + strand at 536.755 kb on + strand at 536.755 kb on + strand at 536.755 kb on + strand at 536.785 kb on + strand at 536.794 kb on + strand at 536.794 kb on + strand at 536.861 kb on + strand, within IAI47_02595 at 536.862 kb on - strand, within IAI47_02595 at 536.862 kb on - strand, within IAI47_02595 at 536.880 kb on + strand, within IAI47_02595 at 536.880 kb on + strand, within IAI47_02595 at 536.880 kb on + strand, within IAI47_02595 at 536.881 kb on - strand, within IAI47_02595 at 536.881 kb on - strand, within IAI47_02595 at 536.881 kb on - strand, within IAI47_02595 at 536.881 kb on - strand, within IAI47_02595 at 536.923 kb on + strand, within IAI47_02595 at 536.950 kb on + strand, within IAI47_02595 at 536.951 kb on - strand, within IAI47_02595 at 536.966 kb on + strand, within IAI47_02595 at 536.967 kb on - strand, within IAI47_02595 at 537.016 kb on + strand, within IAI47_02595 at 537.016 kb on + strand, within IAI47_02595 at 537.016 kb on + strand, within IAI47_02595 at 537.016 kb on + strand, within IAI47_02595 at 537.017 kb on - strand, within IAI47_02595 at 537.031 kb on + strand, within IAI47_02595 at 537.041 kb on + strand, within IAI47_02595 at 537.042 kb on - strand, within IAI47_02595 at 537.062 kb on + strand, within IAI47_02595 at 537.063 kb on - strand, within IAI47_02595 at 537.063 kb on - strand, within IAI47_02595 at 537.063 kb on - strand, within IAI47_02595 at 537.074 kb on + strand, within IAI47_02595 at 537.100 kb on + strand, within IAI47_02595 at 537.100 kb on + strand, within IAI47_02595 at 537.100 kb on + strand, within IAI47_02595 at 537.100 kb on + strand, within IAI47_02595 at 537.101 kb on - strand, within IAI47_02595 at 537.101 kb on - strand, within IAI47_02595 at 537.101 kb on - strand, within IAI47_02595 at 537.124 kb on + strand, within IAI47_02595 at 537.193 kb on + strand, within IAI47_02595 at 537.193 kb on + strand, within IAI47_02595 at 537.193 kb on + strand, within IAI47_02595 at 537.193 kb on + strand, within IAI47_02595 at 537.193 kb on + strand, within IAI47_02595 at 537.193 kb on + strand, within IAI47_02595 at 537.193 kb on + strand, within IAI47_02595 at 537.194 kb on - strand, within IAI47_02595 at 537.194 kb on - strand, within IAI47_02595
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 534,275 + IAI47_02580 0.46 +0.2 534,275 + IAI47_02580 0.46 -0.8 534,275 + IAI47_02580 0.46 +2.1 534,276 - IAI47_02580 0.46 +1.4 534,285 - IAI47_02580 0.47 +1.1 534,285 - IAI47_02580 0.47 +4.9 534,342 + IAI47_02580 0.50 -1.6 534,343 - IAI47_02580 0.50 -2.7 534,343 - IAI47_02580 0.50 +0.3 534,343 - IAI47_02580 0.50 -0.7 534,366 + IAI47_02580 0.51 -2.3 534,366 + IAI47_02580 0.51 +0.7 534,366 + IAI47_02580 0.51 +0.6 534,366 + IAI47_02580 0.51 +1.2 534,366 + IAI47_02580 0.51 +3.5 534,367 - IAI47_02580 0.51 +0.8 534,367 - IAI47_02580 0.51 +0.1 534,367 - IAI47_02580 0.51 -0.5 534,367 - IAI47_02580 0.51 +0.8 534,367 - IAI47_02580 0.51 -3.2 534,376 + IAI47_02580 0.51 -2.5 534,376 + IAI47_02580 0.51 -0.5 534,376 + IAI47_02580 0.51 +3.7 534,376 + IAI47_02580 0.51 +2.4 534,376 + IAI47_02580 0.51 +0.2 534,377 - IAI47_02580 0.51 -2.3 534,377 - IAI47_02580 0.51 +0.8 534,377 - IAI47_02580 0.51 +0.5 534,381 - IAI47_02580 0.52 -0.6 534,381 - IAI47_02580 0.52 -1.5 534,381 - IAI47_02580 0.52 +0.1 534,588 - IAI47_02580 0.63 -1.6 534,611 + IAI47_02580 0.64 +1.2 534,798 + IAI47_02580 0.74 -3.0 534,798 + IAI47_02580 0.74 +1.1 534,798 + IAI47_02580 0.74 +0.6 534,798 + IAI47_02580 0.74 -2.5 534,798 + IAI47_02580 0.74 -2.1 534,799 - IAI47_02580 0.74 +0.6 534,799 - IAI47_02580 0.74 +0.1 534,799 - IAI47_02580 0.74 -0.3 534,799 - IAI47_02580 0.74 +0.1 534,799 - IAI47_02580 0.74 +1.7 534,799 - IAI47_02580 0.74 +0.1 534,871 + IAI47_02580 0.78 -0.4 534,871 + IAI47_02580 0.78 -4.1 534,871 + IAI47_02580 0.78 -2.2 534,872 - IAI47_02580 0.78 -1.6 534,872 - IAI47_02580 0.78 +3.1 534,872 - IAI47_02580 0.78 -1.5 534,946 + IAI47_02580 0.82 -1.8 534,946 + IAI47_02580 0.82 -3.5 534,946 + IAI47_02580 0.82 -0.5 534,947 - IAI47_02580 0.82 +2.0 535,062 + IAI47_02580 0.88 -0.3 535,062 + IAI47_02580 0.88 +2.4 535,062 + IAI47_02580 0.88 -0.2 535,062 + IAI47_02580 0.88 -0.5 535,062 + IAI47_02580 0.88 -0.7 535,062 + IAI47_02580 0.88 -1.3 535,062 + IAI47_02580 0.88 +1.7 535,063 - IAI47_02580 0.88 -2.3 535,063 - IAI47_02580 0.88 -0.5 535,063 - IAI47_02580 0.88 -1.5 535,063 - IAI47_02580 0.88 +1.1 535,067 + IAI47_02580 0.88 +1.5 535,068 - IAI47_02580 0.88 -0.2 535,139 + +0.4 535,139 + -3.4 535,230 + +0.6 535,230 + -4.1 535,230 + +0.3 535,230 + -0.9 535,231 - +0.3 535,231 - -0.2 535,231 - +0.4 535,231 - -1.8 535,231 - -0.5 535,231 - +1.0 535,231 - -0.1 535,233 + +0.6 535,233 + -0.3 535,233 + -2.5 535,233 + +0.3 535,233 + +0.3 535,234 - -2.2 535,234 - -0.9 535,234 - +0.8 535,242 + -1.3 535,242 + +1.0 535,285 + -1.8 535,286 - -1.6 535,368 - +0.7 535,376 - IAI47_02585 0.11 -1.3 535,410 - IAI47_02585 0.14 -0.6 535,413 + IAI47_02585 0.15 -1.6 535,459 + IAI47_02585 0.19 -3.7 535,460 - IAI47_02585 0.19 -1.9 535,605 + IAI47_02585 0.35 +2.7 535,606 - IAI47_02585 0.35 -0.3 535,857 + IAI47_02585 0.61 -0.6 536,043 + IAI47_02585 0.81 +2.3 536,043 + IAI47_02585 0.81 -1.5 536,044 - IAI47_02585 0.81 -0.6 536,122 + IAI47_02585 0.89 +1.0 536,122 + IAI47_02585 0.89 -2.5 536,123 - IAI47_02585 0.89 -1.3 536,189 + -0.9 536,190 - +2.7 536,190 - +0.1 536,207 + +2.7 536,207 + -0.6 536,208 - -0.3 536,231 + +0.7 536,269 + -0.8 536,283 + +2.0 536,283 + +0.0 536,283 + +0.1 536,283 + -0.9 536,283 + +0.1 536,283 + +1.7 536,283 + -0.1 536,283 + +0.1 536,283 + -0.1 536,283 + -0.1 536,284 - +4.4 536,290 + -0.3 536,290 + +1.1 536,290 + -0.4 536,290 + -1.9 536,290 + +1.0 536,290 + -1.6 536,290 + +1.8 536,290 + -0.4 536,290 + +1.1 536,290 + -1.1 536,293 + +0.1 536,293 + -0.6 536,312 + +0.6 536,312 + -1.2 536,312 + -0.3 536,312 + -0.6 536,313 - +1.7 536,325 + +0.5 536,348 + +1.3 536,415 + IAI47_02590 0.27 +4.3 536,418 + IAI47_02590 0.28 -1.3 536,477 + IAI47_02590 0.47 -0.6 536,491 + IAI47_02590 0.51 +0.7 536,491 + IAI47_02590 0.51 -0.3 536,491 + IAI47_02590 0.51 +3.0 536,491 + IAI47_02590 0.51 -1.1 536,493 + IAI47_02590 0.52 +1.9 536,494 - IAI47_02590 0.52 +2.4 536,541 + IAI47_02590 0.67 +3.3 536,547 + IAI47_02590 0.69 +0.8 536,547 + IAI47_02590 0.69 +1.4 536,547 + IAI47_02590 0.69 +2.2 536,547 + IAI47_02590 0.69 +1.5 536,547 + IAI47_02590 0.69 +2.7 536,547 + IAI47_02590 0.69 +1.4 536,547 + IAI47_02590 0.69 -1.5 536,547 + IAI47_02590 0.69 +0.4 536,548 - IAI47_02590 0.70 +0.6 536,575 + IAI47_02590 0.78 +0.5 536,575 + IAI47_02590 0.78 -0.1 536,575 + IAI47_02590 0.78 +0.4 536,575 + IAI47_02590 0.78 +0.1 536,632 - +0.1 536,641 + -2.1 536,654 + +1.7 536,654 + -1.6 536,654 + -0.5 536,654 + +0.8 536,654 + -1.3 536,696 + -2.7 536,696 + +0.2 536,696 + -0.6 536,697 - -0.1 536,697 - +1.0 536,697 - -1.8 536,709 + +0.5 536,711 + +0.5 536,711 + +0.7 536,711 + -0.6 536,712 - -0.7 536,712 - +0.0 536,712 - -0.8 536,712 - -2.5 536,712 - -3.3 536,712 - -1.3 536,712 - -2.5 536,712 - +0.7 536,712 - +0.4 536,755 + -2.1 536,755 + +0.4 536,755 + -0.1 536,755 + +1.6 536,785 + +0.5 536,794 + +0.1 536,794 + -2.5 536,861 + IAI47_02595 0.11 -1.4 536,862 - IAI47_02595 0.11 +1.2 536,862 - IAI47_02595 0.11 +0.1 536,880 + IAI47_02595 0.13 -2.0 536,880 + IAI47_02595 0.13 -0.6 536,880 + IAI47_02595 0.13 +2.5 536,881 - IAI47_02595 0.13 +1.3 536,881 - IAI47_02595 0.13 +1.6 536,881 - IAI47_02595 0.13 +1.3 536,881 - IAI47_02595 0.13 -0.1 536,923 + IAI47_02595 0.16 -1.2 536,950 + IAI47_02595 0.18 -3.5 536,951 - IAI47_02595 0.18 -0.2 536,966 + IAI47_02595 0.20 +0.2 536,967 - IAI47_02595 0.20 -2.2 537,016 + IAI47_02595 0.23 +0.7 537,016 + IAI47_02595 0.23 +0.4 537,016 + IAI47_02595 0.23 +1.3 537,016 + IAI47_02595 0.23 -0.1 537,017 - IAI47_02595 0.24 +1.0 537,031 + IAI47_02595 0.25 +0.5 537,041 + IAI47_02595 0.25 +0.1 537,042 - IAI47_02595 0.26 -0.5 537,062 + IAI47_02595 0.27 +2.6 537,063 - IAI47_02595 0.27 -1.3 537,063 - IAI47_02595 0.27 +1.6 537,063 - IAI47_02595 0.27 -2.3 537,074 + IAI47_02595 0.28 -0.6 537,100 + IAI47_02595 0.30 +0.5 537,100 + IAI47_02595 0.30 -0.4 537,100 + IAI47_02595 0.30 -0.5 537,100 + IAI47_02595 0.30 +0.5 537,101 - IAI47_02595 0.30 -3.2 537,101 - IAI47_02595 0.30 +1.2 537,101 - IAI47_02595 0.30 -1.1 537,124 + IAI47_02595 0.32 -3.1 537,193 + IAI47_02595 0.37 +0.5 537,193 + IAI47_02595 0.37 +0.7 537,193 + IAI47_02595 0.37 +0.4 537,193 + IAI47_02595 0.37 -2.4 537,193 + IAI47_02595 0.37 +0.3 537,193 + IAI47_02595 0.37 +0.8 537,193 + IAI47_02595 0.37 -0.2 537,194 - IAI47_02595 0.37 -1.5 537,194 - IAI47_02595 0.37 +2.0
Or see this region's nucleotide sequence