Experiment: Survival; Treatment=UGA synthetic gw pH7.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_01375 and IAI47_01380 are separated by 293 nucleotides IAI47_01380 and IAI47_01385 are separated by 1 nucleotides IAI47_01385 and IAI47_01390 are separated by 54 nucleotides
IAI47_01375: IAI47_01375 - aldo/keto reductase, at 297,209 to 298,195
_01375
IAI47_01380: IAI47_01380 - hypothetical protein, at 298,489 to 298,686
_01380
IAI47_01385: IAI47_01385 - hypothetical protein, at 298,688 to 299,515
_01385
IAI47_01390: IAI47_01390 - LacI family DNA-binding transcriptional regulator, at 299,570 to 300,589
_01390
Position (kb) 298
299 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 297.509 kb on - strand, within IAI47_01375 at 297.509 kb on - strand, within IAI47_01375 at 297.669 kb on + strand, within IAI47_01375 at 297.669 kb on + strand, within IAI47_01375 at 297.748 kb on - strand, within IAI47_01375 at 297.748 kb on - strand, within IAI47_01375 at 297.807 kb on + strand, within IAI47_01375 at 297.807 kb on + strand, within IAI47_01375 at 297.808 kb on - strand, within IAI47_01375 at 297.808 kb on - strand, within IAI47_01375 at 297.808 kb on - strand, within IAI47_01375 at 297.808 kb on - strand, within IAI47_01375 at 297.808 kb on - strand, within IAI47_01375 at 297.808 kb on - strand, within IAI47_01375 at 297.808 kb on - strand, within IAI47_01375 at 297.808 kb on - strand, within IAI47_01375 at 297.810 kb on + strand, within IAI47_01375 at 297.810 kb on + strand, within IAI47_01375 at 297.810 kb on + strand, within IAI47_01375 at 297.810 kb on + strand, within IAI47_01375 at 297.811 kb on - strand, within IAI47_01375 at 297.811 kb on - strand, within IAI47_01375 at 297.811 kb on - strand, within IAI47_01375 at 297.811 kb on - strand, within IAI47_01375 at 297.811 kb on - strand, within IAI47_01375 at 297.811 kb on - strand, within IAI47_01375 at 297.887 kb on - strand, within IAI47_01375 at 297.913 kb on - strand, within IAI47_01375 at 297.913 kb on - strand, within IAI47_01375 at 297.913 kb on - strand, within IAI47_01375 at 298.029 kb on + strand, within IAI47_01375 at 298.029 kb on + strand, within IAI47_01375 at 298.030 kb on - strand, within IAI47_01375 at 298.030 kb on - strand, within IAI47_01375 at 298.030 kb on - strand, within IAI47_01375 at 298.042 kb on + strand, within IAI47_01375 at 298.043 kb on - strand, within IAI47_01375 at 298.048 kb on + strand, within IAI47_01375 at 298.049 kb on - strand, within IAI47_01375 at 298.049 kb on - strand, within IAI47_01375 at 298.049 kb on - strand, within IAI47_01375 at 298.255 kb on - strand at 298.286 kb on + strand at 298.286 kb on + strand at 298.339 kb on + strand at 298.339 kb on + strand at 298.339 kb on + strand at 298.339 kb on + strand at 298.339 kb on + strand at 298.340 kb on - strand at 298.340 kb on - strand at 298.340 kb on - strand at 298.354 kb on + strand at 298.369 kb on + strand at 298.381 kb on + strand at 298.456 kb on + strand at 298.456 kb on + strand at 298.456 kb on + strand at 298.456 kb on + strand at 298.456 kb on + strand at 298.456 kb on + strand at 298.457 kb on - strand at 298.457 kb on - strand at 298.457 kb on - strand at 298.457 kb on - strand at 298.457 kb on - strand at 298.488 kb on + strand at 298.489 kb on - strand at 298.489 kb on - strand at 298.500 kb on + strand at 298.503 kb on + strand at 298.503 kb on + strand at 298.542 kb on + strand, within IAI47_01380 at 298.542 kb on + strand, within IAI47_01380 at 298.543 kb on - strand, within IAI47_01380 at 298.543 kb on - strand, within IAI47_01380 at 298.543 kb on - strand, within IAI47_01380 at 298.543 kb on - strand, within IAI47_01380 at 298.558 kb on - strand, within IAI47_01380 at 298.593 kb on + strand, within IAI47_01380 at 298.593 kb on + strand, within IAI47_01380 at 298.594 kb on - strand, within IAI47_01380 at 298.594 kb on - strand, within IAI47_01380 at 298.594 kb on - strand, within IAI47_01380 at 298.594 kb on - strand, within IAI47_01380 at 298.594 kb on - strand, within IAI47_01380 at 298.594 kb on - strand, within IAI47_01380 at 298.612 kb on - strand, within IAI47_01380 at 298.669 kb on - strand at 298.669 kb on - strand at 298.674 kb on - strand at 298.682 kb on + strand at 298.682 kb on + strand at 298.682 kb on + strand at 298.683 kb on - strand at 298.683 kb on - strand at 298.683 kb on - strand at 298.683 kb on - strand at 298.683 kb on - strand at 298.684 kb on + strand at 298.684 kb on + strand at 298.684 kb on + strand at 298.684 kb on + strand at 298.684 kb on + strand at 298.684 kb on + strand at 298.684 kb on + strand at 298.684 kb on + strand at 298.684 kb on + strand at 298.684 kb on + strand at 298.684 kb on + strand at 298.684 kb on + strand at 298.684 kb on + strand at 298.684 kb on + strand at 298.685 kb on - strand at 298.685 kb on - strand at 298.685 kb on - strand at 298.685 kb on - strand at 298.685 kb on - strand at 298.685 kb on - strand at 298.685 kb on - strand at 298.685 kb on - strand at 298.685 kb on - strand at 298.685 kb on - strand at 298.685 kb on - strand at 298.685 kb on - strand at 298.685 kb on - strand at 298.685 kb on - strand at 298.685 kb on - strand at 298.685 kb on - strand at 298.685 kb on - strand at 298.685 kb on - strand at 298.701 kb on + strand at 298.702 kb on - strand at 298.702 kb on - strand at 298.702 kb on - strand at 298.705 kb on + strand at 298.705 kb on + strand at 298.705 kb on + strand at 298.705 kb on + strand at 298.705 kb on + strand at 298.706 kb on - strand at 298.706 kb on - strand at 298.706 kb on - strand at 298.706 kb on - strand at 298.706 kb on - strand at 298.706 kb on - strand at 298.728 kb on - strand at 298.734 kb on - strand at 298.734 kb on - strand at 298.734 kb on - strand at 298.793 kb on + strand, within IAI47_01385 at 298.793 kb on + strand, within IAI47_01385 at 298.793 kb on + strand, within IAI47_01385 at 298.793 kb on + strand, within IAI47_01385 at 298.793 kb on + strand, within IAI47_01385 at 298.794 kb on - strand, within IAI47_01385 at 298.794 kb on - strand, within IAI47_01385 at 298.794 kb on - strand, within IAI47_01385 at 298.794 kb on - strand, within IAI47_01385 at 298.811 kb on + strand, within IAI47_01385 at 298.812 kb on - strand, within IAI47_01385 at 298.812 kb on - strand, within IAI47_01385 at 298.812 kb on - strand, within IAI47_01385 at 298.820 kb on - strand, within IAI47_01385 at 298.839 kb on + strand, within IAI47_01385 at 298.840 kb on - strand, within IAI47_01385 at 298.866 kb on + strand, within IAI47_01385 at 298.866 kb on + strand, within IAI47_01385 at 298.866 kb on + strand, within IAI47_01385 at 298.867 kb on - strand, within IAI47_01385 at 298.867 kb on - strand, within IAI47_01385 at 298.867 kb on - strand, within IAI47_01385 at 298.876 kb on + strand, within IAI47_01385 at 298.876 kb on + strand, within IAI47_01385 at 298.942 kb on + strand, within IAI47_01385 at 298.943 kb on - strand, within IAI47_01385 at 299.015 kb on + strand, within IAI47_01385 at 299.016 kb on - strand, within IAI47_01385 at 299.053 kb on - strand, within IAI47_01385 at 299.062 kb on + strand, within IAI47_01385 at 299.063 kb on - strand, within IAI47_01385 at 299.073 kb on - strand, within IAI47_01385 at 299.149 kb on + strand, within IAI47_01385 at 299.149 kb on + strand, within IAI47_01385 at 299.149 kb on + strand, within IAI47_01385 at 299.149 kb on + strand, within IAI47_01385 at 299.149 kb on + strand, within IAI47_01385 at 299.171 kb on + strand, within IAI47_01385 at 299.171 kb on + strand, within IAI47_01385 at 299.171 kb on + strand, within IAI47_01385 at 299.172 kb on - strand, within IAI47_01385 at 299.172 kb on - strand, within IAI47_01385 at 299.172 kb on - strand, within IAI47_01385 at 299.204 kb on + strand, within IAI47_01385 at 299.204 kb on + strand, within IAI47_01385 at 299.204 kb on + strand, within IAI47_01385 at 299.205 kb on - strand, within IAI47_01385 at 299.205 kb on - strand, within IAI47_01385 at 299.205 kb on - strand, within IAI47_01385 at 299.215 kb on + strand, within IAI47_01385 at 299.216 kb on - strand, within IAI47_01385 at 299.287 kb on + strand, within IAI47_01385 at 299.287 kb on + strand, within IAI47_01385 at 299.287 kb on + strand, within IAI47_01385 at 299.287 kb on + strand, within IAI47_01385 at 299.287 kb on + strand, within IAI47_01385 at 299.287 kb on + strand, within IAI47_01385 at 299.288 kb on - strand, within IAI47_01385 at 299.288 kb on - strand, within IAI47_01385 at 299.288 kb on - strand, within IAI47_01385 at 299.288 kb on - strand, within IAI47_01385 at 299.288 kb on - strand, within IAI47_01385 at 299.288 kb on - strand, within IAI47_01385 at 299.288 kb on - strand, within IAI47_01385 at 299.288 kb on - strand, within IAI47_01385 at 299.288 kb on - strand, within IAI47_01385 at 299.288 kb on - strand, within IAI47_01385 at 299.291 kb on + strand, within IAI47_01385 at 299.291 kb on + strand, within IAI47_01385 at 299.291 kb on + strand, within IAI47_01385 at 299.291 kb on + strand, within IAI47_01385 at 299.291 kb on + strand, within IAI47_01385 at 299.292 kb on - strand, within IAI47_01385 at 299.292 kb on - strand, within IAI47_01385 at 299.292 kb on - strand, within IAI47_01385 at 299.292 kb on - strand, within IAI47_01385 at 299.292 kb on - strand, within IAI47_01385 at 299.292 kb on - strand, within IAI47_01385 at 299.292 kb on - strand, within IAI47_01385 at 299.303 kb on + strand, within IAI47_01385 at 299.304 kb on - strand, within IAI47_01385 at 299.382 kb on + strand, within IAI47_01385 at 299.382 kb on + strand, within IAI47_01385 at 299.383 kb on - strand, within IAI47_01385 at 299.383 kb on - strand, within IAI47_01385 at 299.383 kb on - strand, within IAI47_01385 at 299.383 kb on - strand, within IAI47_01385 at 299.383 kb on - strand, within IAI47_01385 at 299.383 kb on - strand, within IAI47_01385 at 299.383 kb on - strand, within IAI47_01385 at 299.491 kb on + strand at 299.491 kb on + strand at 299.491 kb on + strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.514 kb on - strand at 299.578 kb on + strand at 299.591 kb on - strand at 299.611 kb on + strand at 299.622 kb on + strand at 299.622 kb on + strand at 299.622 kb on + strand at 299.623 kb on - strand at 299.623 kb on - strand at 299.623 kb on - strand at 299.623 kb on - strand at 299.657 kb on + strand at 299.657 kb on + strand at 299.657 kb on + strand at 299.657 kb on + strand at 299.657 kb on + strand at 299.657 kb on + strand at 299.657 kb on + strand at 299.658 kb on - strand at 299.658 kb on - strand at 299.658 kb on - strand at 299.658 kb on - strand at 299.658 kb on - strand at 299.658 kb on - strand at 299.658 kb on - strand at 299.658 kb on - strand at 299.658 kb on - strand at 299.658 kb on - strand at 299.658 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 297,509 - IAI47_01375 0.30 +0.9 297,509 - IAI47_01375 0.30 +0.7 297,669 + IAI47_01375 0.47 +0.1 297,669 + IAI47_01375 0.47 -2.1 297,748 - IAI47_01375 0.55 +1.5 297,748 - IAI47_01375 0.55 -0.4 297,807 + IAI47_01375 0.61 +0.4 297,807 + IAI47_01375 0.61 -0.7 297,808 - IAI47_01375 0.61 +1.6 297,808 - IAI47_01375 0.61 +1.1 297,808 - IAI47_01375 0.61 -0.5 297,808 - IAI47_01375 0.61 +0.4 297,808 - IAI47_01375 0.61 -0.8 297,808 - IAI47_01375 0.61 +0.2 297,808 - IAI47_01375 0.61 +1.3 297,808 - IAI47_01375 0.61 +2.7 297,810 + IAI47_01375 0.61 +0.5 297,810 + IAI47_01375 0.61 +2.1 297,810 + IAI47_01375 0.61 -0.2 297,810 + IAI47_01375 0.61 -0.7 297,811 - IAI47_01375 0.61 -3.5 297,811 - IAI47_01375 0.61 -1.7 297,811 - IAI47_01375 0.61 -1.0 297,811 - IAI47_01375 0.61 -0.3 297,811 - IAI47_01375 0.61 +1.3 297,811 - IAI47_01375 0.61 +1.1 297,887 - IAI47_01375 0.69 +0.4 297,913 - IAI47_01375 0.71 +0.7 297,913 - IAI47_01375 0.71 +0.6 297,913 - IAI47_01375 0.71 -0.1 298,029 + IAI47_01375 0.83 -2.1 298,029 + IAI47_01375 0.83 +0.9 298,030 - IAI47_01375 0.83 -1.0 298,030 - IAI47_01375 0.83 -1.1 298,030 - IAI47_01375 0.83 +2.6 298,042 + IAI47_01375 0.84 +0.4 298,043 - IAI47_01375 0.84 +0.1 298,048 + IAI47_01375 0.85 -0.8 298,049 - IAI47_01375 0.85 +0.8 298,049 - IAI47_01375 0.85 +0.1 298,049 - IAI47_01375 0.85 -0.8 298,255 - -0.9 298,286 + +1.7 298,286 + +0.9 298,339 + +0.3 298,339 + -0.0 298,339 + -0.6 298,339 + +0.6 298,339 + -0.6 298,340 - +0.7 298,340 - -0.5 298,340 - -0.5 298,354 + +0.2 298,369 + +2.1 298,381 + +2.5 298,456 + +0.0 298,456 + +0.6 298,456 + -1.0 298,456 + -2.7 298,456 + +1.3 298,456 + +0.5 298,457 - -1.1 298,457 - -1.0 298,457 - +0.7 298,457 - +0.8 298,457 - -2.8 298,488 + -2.3 298,489 - -2.6 298,489 - +1.2 298,500 + +0.2 298,503 + +0.5 298,503 + -1.1 298,542 + IAI47_01380 0.27 +3.2 298,542 + IAI47_01380 0.27 +1.7 298,543 - IAI47_01380 0.27 -0.2 298,543 - IAI47_01380 0.27 +3.7 298,543 - IAI47_01380 0.27 +0.1 298,543 - IAI47_01380 0.27 +0.9 298,558 - IAI47_01380 0.35 +1.6 298,593 + IAI47_01380 0.53 +0.0 298,593 + IAI47_01380 0.53 -0.8 298,594 - IAI47_01380 0.53 -0.8 298,594 - IAI47_01380 0.53 +1.7 298,594 - IAI47_01380 0.53 -0.2 298,594 - IAI47_01380 0.53 +0.8 298,594 - IAI47_01380 0.53 +2.5 298,594 - IAI47_01380 0.53 -0.2 298,612 - IAI47_01380 0.62 +0.4 298,669 - +0.9 298,669 - -0.2 298,674 - +0.6 298,682 + -1.2 298,682 + +0.2 298,682 + -2.2 298,683 - -0.5 298,683 - +0.6 298,683 - +1.7 298,683 - -0.2 298,683 - -0.5 298,684 + -0.4 298,684 + +3.5 298,684 + -0.2 298,684 + +0.1 298,684 + -0.2 298,684 + +0.2 298,684 + -0.2 298,684 + -0.5 298,684 + -4.3 298,684 + -2.1 298,684 + -1.1 298,684 + +0.6 298,684 + +1.3 298,684 + -2.3 298,685 - +2.1 298,685 - -3.3 298,685 - +0.2 298,685 - +0.6 298,685 - +2.2 298,685 - -0.8 298,685 - -0.9 298,685 - +0.5 298,685 - +1.9 298,685 - +0.8 298,685 - +1.2 298,685 - +0.7 298,685 - +0.9 298,685 - -0.8 298,685 - +0.7 298,685 - +0.6 298,685 - +0.5 298,685 - -1.3 298,701 + -1.5 298,702 - +0.3 298,702 - -0.6 298,702 - +1.4 298,705 + -0.5 298,705 + +1.3 298,705 + +1.1 298,705 + +0.8 298,705 + +1.2 298,706 - +1.1 298,706 - +0.5 298,706 - -0.3 298,706 - -0.6 298,706 - -0.8 298,706 - -1.3 298,728 - -0.6 298,734 - -0.1 298,734 - -1.7 298,734 - -1.5 298,793 + IAI47_01385 0.13 +1.4 298,793 + IAI47_01385 0.13 +1.6 298,793 + IAI47_01385 0.13 -0.3 298,793 + IAI47_01385 0.13 +0.2 298,793 + IAI47_01385 0.13 -0.6 298,794 - IAI47_01385 0.13 +2.6 298,794 - IAI47_01385 0.13 -0.1 298,794 - IAI47_01385 0.13 +0.8 298,794 - IAI47_01385 0.13 -1.9 298,811 + IAI47_01385 0.15 +0.7 298,812 - IAI47_01385 0.15 -0.2 298,812 - IAI47_01385 0.15 +2.0 298,812 - IAI47_01385 0.15 -3.1 298,820 - IAI47_01385 0.16 -4.0 298,839 + IAI47_01385 0.18 -0.3 298,840 - IAI47_01385 0.18 -1.3 298,866 + IAI47_01385 0.21 -1.4 298,866 + IAI47_01385 0.21 +2.1 298,866 + IAI47_01385 0.21 -1.1 298,867 - IAI47_01385 0.22 -0.8 298,867 - IAI47_01385 0.22 +1.7 298,867 - IAI47_01385 0.22 +0.2 298,876 + IAI47_01385 0.23 +0.2 298,876 + IAI47_01385 0.23 -0.5 298,942 + IAI47_01385 0.31 -0.5 298,943 - IAI47_01385 0.31 +0.5 299,015 + IAI47_01385 0.39 -0.6 299,016 - IAI47_01385 0.40 +0.8 299,053 - IAI47_01385 0.44 +0.6 299,062 + IAI47_01385 0.45 -0.8 299,063 - IAI47_01385 0.45 -2.4 299,073 - IAI47_01385 0.46 -1.9 299,149 + IAI47_01385 0.56 -0.6 299,149 + IAI47_01385 0.56 +0.3 299,149 + IAI47_01385 0.56 +0.2 299,149 + IAI47_01385 0.56 -0.1 299,149 + IAI47_01385 0.56 -0.1 299,171 + IAI47_01385 0.58 -0.9 299,171 + IAI47_01385 0.58 -0.1 299,171 + IAI47_01385 0.58 +2.2 299,172 - IAI47_01385 0.58 +1.3 299,172 - IAI47_01385 0.58 -0.6 299,172 - IAI47_01385 0.58 +0.3 299,204 + IAI47_01385 0.62 +0.7 299,204 + IAI47_01385 0.62 -0.1 299,204 + IAI47_01385 0.62 -1.4 299,205 - IAI47_01385 0.62 -3.3 299,205 - IAI47_01385 0.62 -3.2 299,205 - IAI47_01385 0.62 +1.0 299,215 + IAI47_01385 0.64 -0.8 299,216 - IAI47_01385 0.64 -1.0 299,287 + IAI47_01385 0.72 -1.6 299,287 + IAI47_01385 0.72 -2.1 299,287 + IAI47_01385 0.72 -1.8 299,287 + IAI47_01385 0.72 +1.3 299,287 + IAI47_01385 0.72 +0.2 299,287 + IAI47_01385 0.72 -1.3 299,288 - IAI47_01385 0.72 -0.1 299,288 - IAI47_01385 0.72 -0.2 299,288 - IAI47_01385 0.72 -1.7 299,288 - IAI47_01385 0.72 +0.6 299,288 - IAI47_01385 0.72 +0.3 299,288 - IAI47_01385 0.72 +0.5 299,288 - IAI47_01385 0.72 -3.1 299,288 - IAI47_01385 0.72 -2.1 299,288 - IAI47_01385 0.72 +0.2 299,288 - IAI47_01385 0.72 +0.5 299,291 + IAI47_01385 0.73 +1.9 299,291 + IAI47_01385 0.73 +0.3 299,291 + IAI47_01385 0.73 +0.8 299,291 + IAI47_01385 0.73 +1.0 299,291 + IAI47_01385 0.73 -1.5 299,292 - IAI47_01385 0.73 +0.7 299,292 - IAI47_01385 0.73 +1.1 299,292 - IAI47_01385 0.73 -0.3 299,292 - IAI47_01385 0.73 +0.2 299,292 - IAI47_01385 0.73 +1.5 299,292 - IAI47_01385 0.73 -1.1 299,292 - IAI47_01385 0.73 -0.2 299,303 + IAI47_01385 0.74 +2.2 299,304 - IAI47_01385 0.74 -0.3 299,382 + IAI47_01385 0.84 -0.0 299,382 + IAI47_01385 0.84 +1.2 299,383 - IAI47_01385 0.84 +0.7 299,383 - IAI47_01385 0.84 +0.2 299,383 - IAI47_01385 0.84 -1.2 299,383 - IAI47_01385 0.84 +1.4 299,383 - IAI47_01385 0.84 +1.7 299,383 - IAI47_01385 0.84 -0.6 299,383 - IAI47_01385 0.84 +0.3 299,491 + -0.5 299,491 + -0.9 299,491 + +0.5 299,492 - -4.4 299,492 - -0.3 299,492 - +1.0 299,492 - +0.7 299,514 - -1.6 299,578 + +1.5 299,591 - -0.3 299,611 + +0.2 299,622 + -0.7 299,622 + +0.8 299,622 + -0.6 299,623 - -2.8 299,623 - +0.4 299,623 - +1.6 299,623 - +0.0 299,657 + +1.1 299,657 + -1.6 299,657 + -0.1 299,657 + +0.3 299,657 + -1.1 299,657 + +1.3 299,657 + -1.2 299,658 - +0.8 299,658 - +0.8 299,658 - +1.3 299,658 - +0.3 299,658 - +0.3 299,658 - +0.1 299,658 - +0.7 299,658 - +1.1 299,658 - -0.1 299,658 - +0.0 299,658 - -0.9
Or see this region's nucleotide sequence