Experiment: ALP with sodium fluoride 30 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2521 and Dsui_2522 are separated by 85 nucleotides Dsui_2522 and Dsui_2523 are separated by 142 nucleotides Dsui_2523 and Dsui_2524 are separated by 49 nucleotides Dsui_2524 and Dsui_2525 overlap by 20 nucleotides Dsui_2525 and Dsui_2526 are separated by 191 nucleotides
Dsui_2521: Dsui_2521 - cysteine synthase A, at 2,672,738 to 2,673,724
_2521
Dsui_2522: Dsui_2522 - hypothetical protein, at 2,673,810 to 2,673,932
_2522
Dsui_2523: Dsui_2523 - hypothetical protein, at 2,674,075 to 2,674,617
_2523
Dsui_2524: Dsui_2524 - putative conserved small protein, at 2,674,667 to 2,675,002
_2524
Dsui_2525: Dsui_2525 - phage-related protein, at 2,674,983 to 2,675,360
_2525
Dsui_2526: Dsui_2526 - site-specific recombinase, DNA invertase Pin, at 2,675,552 to 2,676,145
_2526
Position (kb)
2674
2675 Strain fitness (log2 ratio)
-2
-1
0
1 at 2673.086 kb on - strand, within Dsui_2521 at 2673.102 kb on - strand, within Dsui_2521 at 2673.123 kb on - strand, within Dsui_2521 at 2673.165 kb on + strand, within Dsui_2521 at 2673.166 kb on + strand, within Dsui_2521 at 2673.209 kb on - strand, within Dsui_2521 at 2673.260 kb on + strand, within Dsui_2521 at 2673.268 kb on - strand, within Dsui_2521 at 2673.268 kb on - strand, within Dsui_2521 at 2673.375 kb on + strand, within Dsui_2521 at 2673.431 kb on + strand at 2673.444 kb on - strand, within Dsui_2521 at 2673.473 kb on - strand, within Dsui_2521 at 2673.489 kb on + strand, within Dsui_2521 at 2673.499 kb on + strand, within Dsui_2521 at 2673.675 kb on + strand at 2673.675 kb on + strand at 2673.683 kb on - strand at 2673.718 kb on + strand at 2673.718 kb on + strand at 2673.726 kb on - strand at 2673.726 kb on - strand at 2673.726 kb on - strand at 2673.726 kb on - strand at 2673.726 kb on - strand at 2673.726 kb on - strand at 2673.726 kb on - strand at 2673.739 kb on + strand at 2673.749 kb on + strand at 2673.749 kb on + strand at 2673.749 kb on + strand at 2673.749 kb on + strand at 2673.757 kb on - strand at 2673.757 kb on - strand at 2673.757 kb on - strand at 2673.757 kb on - strand at 2673.757 kb on - strand at 2673.830 kb on - strand, within Dsui_2522 at 2673.850 kb on + strand, within Dsui_2522 at 2673.850 kb on + strand, within Dsui_2522 at 2673.911 kb on - strand, within Dsui_2522 at 2673.960 kb on + strand at 2673.986 kb on + strand at 2674.132 kb on + strand, within Dsui_2523 at 2674.146 kb on + strand, within Dsui_2523 at 2674.146 kb on + strand, within Dsui_2523 at 2674.183 kb on + strand, within Dsui_2523 at 2674.272 kb on + strand, within Dsui_2523 at 2674.353 kb on + strand, within Dsui_2523 at 2674.366 kb on - strand, within Dsui_2523 at 2674.373 kb on + strand, within Dsui_2523 at 2674.404 kb on - strand, within Dsui_2523 at 2674.503 kb on + strand, within Dsui_2523 at 2675.074 kb on - strand, within Dsui_2525 at 2675.109 kb on + strand, within Dsui_2525 at 2675.151 kb on - strand, within Dsui_2525 at 2675.255 kb on - strand, within Dsui_2525 at 2675.342 kb on + strand at 2675.350 kb on - strand at 2675.368 kb on + strand at 2675.452 kb on - strand at 2675.546 kb on - strand at 2675.569 kb on - strand at 2675.590 kb on - strand at 2675.593 kb on + strand at 2675.611 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with sodium fluoride 30 mM remove 2,673,086 - Dsui_2521 0.35 -0.0 2,673,102 - Dsui_2521 0.37 +1.1 2,673,123 - Dsui_2521 0.39 +0.0 2,673,165 + Dsui_2521 0.43 -0.8 2,673,166 + Dsui_2521 0.43 -0.8 2,673,209 - Dsui_2521 0.48 -1.3 2,673,260 + Dsui_2521 0.53 -1.2 2,673,268 - Dsui_2521 0.54 -0.3 2,673,268 - Dsui_2521 0.54 -0.2 2,673,375 + Dsui_2521 0.65 -0.8 2,673,431 + +0.8 2,673,444 - Dsui_2521 0.72 -0.4 2,673,473 - Dsui_2521 0.74 +0.1 2,673,489 + Dsui_2521 0.76 +0.8 2,673,499 + Dsui_2521 0.77 -0.2 2,673,675 + +0.8 2,673,675 + -0.2 2,673,683 - -0.2 2,673,718 + -0.8 2,673,718 + +0.8 2,673,726 - -0.2 2,673,726 - -0.5 2,673,726 - -0.6 2,673,726 - -0.0 2,673,726 - -0.2 2,673,726 - -0.2 2,673,726 - -0.2 2,673,739 + -0.2 2,673,749 + -0.2 2,673,749 + +0.8 2,673,749 + +0.8 2,673,749 + -1.6 2,673,757 - -0.2 2,673,757 - -0.3 2,673,757 - -0.5 2,673,757 - -0.6 2,673,757 - -0.2 2,673,830 - Dsui_2522 0.16 -2.4 2,673,850 + Dsui_2522 0.33 -0.8 2,673,850 + Dsui_2522 0.33 -1.2 2,673,911 - Dsui_2522 0.82 -0.3 2,673,960 + +0.8 2,673,986 + +0.8 2,674,132 + Dsui_2523 0.10 -1.2 2,674,146 + Dsui_2523 0.13 -1.2 2,674,146 + Dsui_2523 0.13 -1.6 2,674,183 + Dsui_2523 0.20 -0.2 2,674,272 + Dsui_2523 0.36 +0.8 2,674,353 + Dsui_2523 0.51 -0.2 2,674,366 - Dsui_2523 0.54 -0.1 2,674,373 + Dsui_2523 0.55 -0.2 2,674,404 - Dsui_2523 0.61 -0.5 2,674,503 + Dsui_2523 0.79 -0.2 2,675,074 - Dsui_2525 0.24 +0.1 2,675,109 + Dsui_2525 0.33 -0.2 2,675,151 - Dsui_2525 0.44 -0.5 2,675,255 - Dsui_2525 0.72 -0.2 2,675,342 + -1.8 2,675,350 - -0.5 2,675,368 + +0.8 2,675,452 - +0.8 2,675,546 - -0.2 2,675,569 - -0.2 2,675,590 - -0.2 2,675,593 + -0.0 2,675,611 - -0.2
Or see this region's nucleotide sequence