Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16700

Experiment: succinate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16690 and BWI76_RS16695 overlap by 4 nucleotidesBWI76_RS16695 and BWI76_RS16700 overlap by 4 nucleotidesBWI76_RS16700 and BWI76_RS16705 are separated by 74 nucleotides BWI76_RS16690: BWI76_RS16690 - peptide ABC transporter permease, at 3,454,601 to 3,455,548 _RS16690 BWI76_RS16695: BWI76_RS16695 - dipeptide/oligopeptide/nickel ABC transporter ATP-binding protein, at 3,455,545 to 3,456,525 _RS16695 BWI76_RS16700: BWI76_RS16700 - ABC transporter ATP-binding protein, at 3,456,522 to 3,457,487 _RS16700 BWI76_RS16705: BWI76_RS16705 - glucose dehydrogenase, at 3,457,562 to 3,459,967 _RS16705 Position (kb) 3456 3457 3458Strain fitness (log2 ratio) -1 0 1at 3455.667 kb on - strand, within BWI76_RS16695at 3455.711 kb on - strand, within BWI76_RS16695at 3455.942 kb on + strandat 3455.950 kb on - strand, within BWI76_RS16695at 3456.156 kb on + strand, within BWI76_RS16695at 3456.200 kb on - strand, within BWI76_RS16695at 3456.226 kb on + strand, within BWI76_RS16695at 3456.433 kb on + strandat 3456.441 kb on - strandat 3457.530 kb on - strandat 3457.580 kb on + strandat 3457.772 kb on + strandat 3457.901 kb on - strand, within BWI76_RS16705at 3458.352 kb on + strand, within BWI76_RS16705

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Per-strain Table

Position Strand Gene LocusTag Fraction succinate (C)
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3,455,667 - BWI76_RS16695 0.12 +0.3
3,455,711 - BWI76_RS16695 0.17 -0.2
3,455,942 + +1.4
3,455,950 - BWI76_RS16695 0.41 -0.5
3,456,156 + BWI76_RS16695 0.62 -0.6
3,456,200 - BWI76_RS16695 0.67 +0.0
3,456,226 + BWI76_RS16695 0.69 -0.2
3,456,433 + -0.7
3,456,441 - -0.7
3,457,530 - -0.2
3,457,580 + -0.2
3,457,772 + +0.9
3,457,901 - BWI76_RS16705 0.14 +0.4
3,458,352 + BWI76_RS16705 0.33 -1.4

Or see this region's nucleotide sequence