Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS15415

Experiment: succinate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS15405 and BWI76_RS15410 are separated by 139 nucleotidesBWI76_RS15410 and BWI76_RS15415 are separated by 386 nucleotidesBWI76_RS15415 and BWI76_RS15420 are separated by 209 nucleotides BWI76_RS15405: BWI76_RS15405 - diguanylate phosphodiesterase, at 3,226,983 to 3,228,200 _RS15405 BWI76_RS15410: BWI76_RS15410 - N-acetyltransferase, at 3,228,340 to 3,228,774 _RS15410 BWI76_RS15415: BWI76_RS15415 - LacI family transcriptional regulator, at 3,229,161 to 3,230,231 _RS15415 BWI76_RS15420: BWI76_RS15420 - beta-D-galactosidase, at 3,230,441 to 3,233,548 _RS15420 Position (kb) 3229 3230 3231Strain fitness (log2 ratio) -2 -1 0 1at 3228.186 kb on - strandat 3228.197 kb on + strandat 3228.197 kb on + strandat 3228.197 kb on + strandat 3228.205 kb on - strandat 3228.205 kb on - strandat 3228.234 kb on - strandat 3228.236 kb on + strandat 3228.280 kb on - strandat 3228.298 kb on - strandat 3228.314 kb on - strandat 3228.513 kb on + strand, within BWI76_RS15410at 3228.528 kb on - strand, within BWI76_RS15410at 3228.534 kb on - strand, within BWI76_RS15410at 3228.665 kb on + strand, within BWI76_RS15410at 3228.690 kb on + strand, within BWI76_RS15410at 3229.188 kb on - strandat 3229.188 kb on - strandat 3229.241 kb on - strandat 3229.300 kb on + strand, within BWI76_RS15415at 3229.379 kb on + strand, within BWI76_RS15415at 3229.429 kb on - strand, within BWI76_RS15415at 3229.594 kb on - strand, within BWI76_RS15415at 3230.228 kb on + strandat 3230.319 kb on - strandat 3230.489 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction succinate (C)
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3,228,186 - +0.5
3,228,197 + -0.5
3,228,197 + -0.8
3,228,197 + -0.2
3,228,205 - -0.2
3,228,205 - -0.2
3,228,234 - +0.3
3,228,236 + -2.0
3,228,280 - -2.0
3,228,298 - +1.3
3,228,314 - -0.3
3,228,513 + BWI76_RS15410 0.40 -1.8
3,228,528 - BWI76_RS15410 0.43 -0.4
3,228,534 - BWI76_RS15410 0.45 +0.3
3,228,665 + BWI76_RS15410 0.75 +0.3
3,228,690 + BWI76_RS15410 0.80 -0.8
3,229,188 - -0.4
3,229,188 - -0.4
3,229,241 - -0.3
3,229,300 + BWI76_RS15415 0.13 -0.7
3,229,379 + BWI76_RS15415 0.20 +0.8
3,229,429 - BWI76_RS15415 0.25 +0.4
3,229,594 - BWI76_RS15415 0.40 -0.1
3,230,228 + +1.5
3,230,319 - -1.1
3,230,489 + -0.3

Or see this region's nucleotide sequence