Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS10225 and AZOBR_RS10230 are separated by 140 nucleotides AZOBR_RS10230 and AZOBR_RS10235 are separated by 43 nucleotides
AZOBR_RS10225: AZOBR_RS10225 - diaminopimelate decarboxylase, at 2,135,526 to 2,136,800
_RS10225
AZOBR_RS10230: AZOBR_RS10230 - homospermidine synthase, at 2,136,941 to 2,138,359
_RS10230
AZOBR_RS10235: AZOBR_RS10235 - cobalamin biosynthesis protein CobD, at 2,138,403 to 2,139,365
_RS10235
Position (kb)
2136
2137
2138
2139 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2135.941 kb on - strand, within AZOBR_RS10225 at 2136.064 kb on - strand, within AZOBR_RS10225 at 2136.064 kb on - strand, within AZOBR_RS10225 at 2136.192 kb on + strand, within AZOBR_RS10225 at 2136.582 kb on + strand, within AZOBR_RS10225 at 2136.582 kb on + strand, within AZOBR_RS10225 at 2136.582 kb on + strand, within AZOBR_RS10225 at 2136.583 kb on - strand, within AZOBR_RS10225 at 2136.583 kb on - strand, within AZOBR_RS10225 at 2136.583 kb on - strand, within AZOBR_RS10225 at 2136.775 kb on - strand at 2136.798 kb on + strand at 2136.798 kb on + strand at 2136.905 kb on + strand at 2136.906 kb on - strand at 2136.938 kb on + strand at 2136.938 kb on + strand at 2136.938 kb on + strand at 2136.938 kb on + strand at 2136.939 kb on - strand at 2136.939 kb on - strand at 2137.152 kb on - strand, within AZOBR_RS10230 at 2137.152 kb on - strand, within AZOBR_RS10230 at 2137.262 kb on + strand, within AZOBR_RS10230 at 2137.262 kb on + strand, within AZOBR_RS10230 at 2137.298 kb on + strand, within AZOBR_RS10230 at 2137.337 kb on + strand, within AZOBR_RS10230 at 2137.337 kb on + strand, within AZOBR_RS10230 at 2137.338 kb on - strand, within AZOBR_RS10230 at 2137.736 kb on + strand, within AZOBR_RS10230 at 2137.736 kb on + strand, within AZOBR_RS10230 at 2137.844 kb on + strand, within AZOBR_RS10230 at 2137.845 kb on - strand, within AZOBR_RS10230 at 2137.847 kb on + strand, within AZOBR_RS10230 at 2137.847 kb on + strand, within AZOBR_RS10230 at 2137.847 kb on + strand, within AZOBR_RS10230 at 2137.877 kb on + strand, within AZOBR_RS10230 at 2137.895 kb on + strand, within AZOBR_RS10230 at 2137.959 kb on - strand, within AZOBR_RS10230 at 2137.959 kb on - strand, within AZOBR_RS10230 at 2138.051 kb on + strand, within AZOBR_RS10230 at 2138.052 kb on - strand, within AZOBR_RS10230 at 2138.102 kb on + strand, within AZOBR_RS10230 at 2138.238 kb on - strand at 2138.833 kb on + strand, within AZOBR_RS10235 at 2138.834 kb on - strand, within AZOBR_RS10235 at 2139.251 kb on + strand, within AZOBR_RS10235 at 2139.292 kb on + strand at 2139.293 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days remove 2,135,941 - AZOBR_RS10225 0.33 -0.6 2,136,064 - AZOBR_RS10225 0.42 +0.7 2,136,064 - AZOBR_RS10225 0.42 +0.4 2,136,192 + AZOBR_RS10225 0.52 +0.3 2,136,582 + AZOBR_RS10225 0.83 +0.9 2,136,582 + AZOBR_RS10225 0.83 +0.1 2,136,582 + AZOBR_RS10225 0.83 +0.0 2,136,583 - AZOBR_RS10225 0.83 +1.0 2,136,583 - AZOBR_RS10225 0.83 +0.4 2,136,583 - AZOBR_RS10225 0.83 +2.0 2,136,775 - -0.4 2,136,798 + +0.2 2,136,798 + +0.8 2,136,905 + +0.7 2,136,906 - +0.2 2,136,938 + +0.5 2,136,938 + +0.2 2,136,938 + -1.3 2,136,938 + -0.2 2,136,939 - -1.2 2,136,939 - -1.0 2,137,152 - AZOBR_RS10230 0.15 +0.6 2,137,152 - AZOBR_RS10230 0.15 -0.4 2,137,262 + AZOBR_RS10230 0.23 -0.5 2,137,262 + AZOBR_RS10230 0.23 +1.2 2,137,298 + AZOBR_RS10230 0.25 +0.6 2,137,337 + AZOBR_RS10230 0.28 -0.1 2,137,337 + AZOBR_RS10230 0.28 -2.5 2,137,338 - AZOBR_RS10230 0.28 -1.9 2,137,736 + AZOBR_RS10230 0.56 -0.6 2,137,736 + AZOBR_RS10230 0.56 +0.2 2,137,844 + AZOBR_RS10230 0.64 -0.4 2,137,845 - AZOBR_RS10230 0.64 -3.2 2,137,847 + AZOBR_RS10230 0.64 -0.4 2,137,847 + AZOBR_RS10230 0.64 -0.5 2,137,847 + AZOBR_RS10230 0.64 +0.5 2,137,877 + AZOBR_RS10230 0.66 +0.2 2,137,895 + AZOBR_RS10230 0.67 -0.3 2,137,959 - AZOBR_RS10230 0.72 +0.2 2,137,959 - AZOBR_RS10230 0.72 -0.6 2,138,051 + AZOBR_RS10230 0.78 -1.2 2,138,052 - AZOBR_RS10230 0.78 +0.1 2,138,102 + AZOBR_RS10230 0.82 -1.2 2,138,238 - -0.2 2,138,833 + AZOBR_RS10235 0.45 -2.1 2,138,834 - AZOBR_RS10235 0.45 -1.0 2,139,251 + AZOBR_RS10235 0.88 +0.5 2,139,292 + -2.6 2,139,293 - -0.1
Or see this region's nucleotide sequence