Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS08285 and AZOBR_RS08290 are separated by 235 nucleotides AZOBR_RS08290 and AZOBR_RS08295 are separated by 85 nucleotides AZOBR_RS08295 and AZOBR_RS08300 are separated by 19 nucleotides AZOBR_RS08300 and AZOBR_RS08305 are separated by 42 nucleotides AZOBR_RS08305 and AZOBR_RS08310 are separated by 90 nucleotides
AZOBR_RS08285: AZOBR_RS08285 - histidine kinase, at 1,743,774 to 1,745,237
_RS08285
AZOBR_RS08290: AZOBR_RS08290 - hypothetical protein, at 1,745,473 to 1,745,688
_RS08290
AZOBR_RS08295: AZOBR_RS08295 - membrane protein, at 1,745,774 to 1,746,214
_RS08295
AZOBR_RS08300: AZOBR_RS08300 - pseudouridine synthase, at 1,746,234 to 1,746,923
_RS08300
AZOBR_RS08305: AZOBR_RS08305 - oxidoreductase, at 1,746,966 to 1,747,676
_RS08305
AZOBR_RS08310: AZOBR_RS08310 - amidophosphoribosyltransferase, at 1,747,767 to 1,749,215
_RS08310
Position (kb) 1746
1747 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 1745.309 kb on + strand at 1745.310 kb on - strand at 1745.365 kb on + strand at 1745.366 kb on - strand at 1745.721 kb on - strand at 1745.721 kb on - strand at 1745.721 kb on - strand at 1745.790 kb on + strand at 1745.790 kb on + strand at 1745.791 kb on - strand at 1745.791 kb on - strand at 1745.801 kb on + strand at 1745.830 kb on - strand, within AZOBR_RS08295 at 1746.011 kb on + strand, within AZOBR_RS08295 at 1746.150 kb on - strand, within AZOBR_RS08295 at 1746.213 kb on - strand at 1746.284 kb on + strand at 1746.649 kb on + strand, within AZOBR_RS08300 at 1746.649 kb on + strand, within AZOBR_RS08300 at 1746.649 kb on + strand, within AZOBR_RS08300 at 1746.649 kb on + strand, within AZOBR_RS08300 at 1746.650 kb on - strand, within AZOBR_RS08300 at 1746.650 kb on - strand, within AZOBR_RS08300 at 1746.889 kb on + strand at 1747.165 kb on + strand, within AZOBR_RS08305 at 1747.165 kb on + strand, within AZOBR_RS08305 at 1747.165 kb on + strand, within AZOBR_RS08305 at 1747.165 kb on + strand, within AZOBR_RS08305 at 1747.367 kb on - strand at 1747.450 kb on + strand, within AZOBR_RS08305
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days remove 1,745,309 + +0.8 1,745,310 - +2.1 1,745,365 + +0.1 1,745,366 - -0.2 1,745,721 - +0.6 1,745,721 - +0.2 1,745,721 - -0.4 1,745,790 + +2.1 1,745,790 + -0.0 1,745,791 - +0.7 1,745,791 - -1.3 1,745,801 + -0.7 1,745,830 - AZOBR_RS08295 0.13 -0.8 1,746,011 + AZOBR_RS08295 0.54 +0.0 1,746,150 - AZOBR_RS08295 0.85 +0.4 1,746,213 - -1.2 1,746,284 + -0.5 1,746,649 + AZOBR_RS08300 0.60 +0.6 1,746,649 + AZOBR_RS08300 0.60 +0.3 1,746,649 + AZOBR_RS08300 0.60 +0.2 1,746,649 + AZOBR_RS08300 0.60 +0.8 1,746,650 - AZOBR_RS08300 0.60 +0.1 1,746,650 - AZOBR_RS08300 0.60 -2.9 1,746,889 + -1.3 1,747,165 + AZOBR_RS08305 0.28 -4.7 1,747,165 + AZOBR_RS08305 0.28 -2.4 1,747,165 + AZOBR_RS08305 0.28 -3.4 1,747,165 + AZOBR_RS08305 0.28 -4.1 1,747,367 - -3.2 1,747,450 + AZOBR_RS08305 0.68 -3.0
Or see this region's nucleotide sequence