Experiment: Carbon source D,L-Lactate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3407 and panB are separated by 56 nucleotides panB and panC are separated by 76 nucleotides panC and Shewana3_3410 are separated by 291 nucleotides Shewana3_3410 and Shewana3_3411 are separated by 61 nucleotides
Shewana3_3407: Shewana3_3407 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (RefSeq), at 4,076,228 to 4,076,716
_3407
Shewana3_3408: panB - 3-methyl-2-oxobutanoate hydroxymethyltransferase (RefSeq), at 4,076,773 to 4,077,567
panB
Shewana3_3409: panC - pantoate--beta-alanine ligase (RefSeq), at 4,077,644 to 4,078,489
panC
Shewana3_3410: Shewana3_3410 - hypothetical protein (RefSeq), at 4,078,781 to 4,079,302
_3410
Shewana3_3411: Shewana3_3411 - peptidase S8 and S53, subtilisin, kexin, sedolisin (RefSeq), at 4,079,364 to 4,081,871
_3411
Position (kb)
4077
4078
4079 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4076.890 kb on + strand, within panB at 4077.047 kb on + strand, within panB at 4077.081 kb on + strand, within panB at 4077.163 kb on + strand, within panB at 4077.433 kb on + strand, within panB at 4077.443 kb on + strand, within panB at 4078.547 kb on - strand at 4078.547 kb on - strand at 4078.547 kb on - strand at 4078.547 kb on - strand at 4078.552 kb on - strand at 4078.665 kb on + strand at 4079.051 kb on - strand, within Shewana3_3410 at 4079.370 kb on - strand at 4079.370 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source D,L-Lactate 10 mM remove 4,076,890 + panB Shewana3_3408 0.15 -2.3 4,077,047 + panB Shewana3_3408 0.34 -0.9 4,077,081 + panB Shewana3_3408 0.39 -3.3 4,077,163 + panB Shewana3_3408 0.49 -4.5 4,077,433 + panB Shewana3_3408 0.83 -2.8 4,077,443 + panB Shewana3_3408 0.84 -3.4 4,078,547 - +0.1 4,078,547 - +0.5 4,078,547 - +0.1 4,078,547 - -0.2 4,078,552 - +0.4 4,078,665 + -0.5 4,079,051 - Shewana3_3410 0.52 +0.6 4,079,370 - +0.2 4,079,370 - -0.1
Or see this region's nucleotide sequence