Experiment: Carbon source D,L-Lactate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt metG and Shewana3_1761 are separated by 160 nucleotides Shewana3_1761 and Shewana3_1762 are separated by 11 nucleotides Shewana3_1762 and dcd are separated by 29 nucleotides
Shewana3_1760: metG - methionyl-tRNA synthetase (RefSeq), at 2,073,887 to 2,075,917
metG
Shewana3_1761: Shewana3_1761 - ATP-binding Mrp/Nbp35 family protein (RefSeq), at 2,076,078 to 2,077,193
_1761
Shewana3_1762: Shewana3_1762 - uridine kinase (RefSeq), at 2,077,205 to 2,077,843
_1762
Shewana3_1763: dcd - deoxycytidine triphosphate deaminase (RefSeq), at 2,077,873 to 2,078,454
dcd
Position (kb)
2076
2077
2078 Strain fitness (log2 ratio)
-2
-1
0
1 at 2076.174 kb on + strand at 2076.184 kb on + strand at 2076.215 kb on + strand, within Shewana3_1761 at 2076.239 kb on + strand, within Shewana3_1761 at 2076.244 kb on + strand, within Shewana3_1761 at 2076.372 kb on + strand, within Shewana3_1761 at 2076.459 kb on + strand, within Shewana3_1761 at 2076.565 kb on - strand, within Shewana3_1761 at 2076.580 kb on + strand, within Shewana3_1761 at 2076.670 kb on + strand, within Shewana3_1761 at 2076.837 kb on + strand, within Shewana3_1761 at 2077.087 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source D,L-Lactate 10 mM remove 2,076,174 + -0.2 2,076,184 + -0.6 2,076,215 + Shewana3_1761 0.12 -1.2 2,076,239 + Shewana3_1761 0.14 -1.5 2,076,244 + Shewana3_1761 0.15 -2.0 2,076,372 + Shewana3_1761 0.26 -2.3 2,076,459 + Shewana3_1761 0.34 -1.5 2,076,565 - Shewana3_1761 0.44 -0.8 2,076,580 + Shewana3_1761 0.45 -1.1 2,076,670 + Shewana3_1761 0.53 +0.4 2,076,837 + Shewana3_1761 0.68 -1.0 2,077,087 + -0.3
Or see this region's nucleotide sequence