Experiment: Carbon source D,L-Lactate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hemC and cyaA are separated by 202 nucleotides cyaA and Shewana3_0388 are separated by 39 nucleotides Shewana3_0388 and Shewana3_0389 are separated by 67 nucleotides Shewana3_0389 and Shewana3_0390 are separated by 42 nucleotides
Shewana3_0386: hemC - porphobilinogen deaminase (RefSeq), at 446,502 to 447,434
hemC
Shewana3_0387: cyaA - adenylate cyclase (RefSeq), at 447,637 to 450,051
cyaA
Shewana3_0388: Shewana3_0388 - frataxin family protein (RefSeq), at 450,091 to 450,420
_0388
Shewana3_0389: Shewana3_0389 - hypothetical protein (RefSeq), at 450,488 to 450,682
_0389
Shewana3_0390: Shewana3_0390 - diaminopimelate decarboxylase (RefSeq), at 450,725 to 451,969
_0390
Position (kb) 447
448
449
450
451 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 447.707 kb on - strand at 447.738 kb on - strand at 447.852 kb on - strand at 447.941 kb on - strand, within cyaA at 447.977 kb on - strand, within cyaA at 447.986 kb on + strand, within cyaA at 447.994 kb on - strand, within cyaA at 447.994 kb on - strand, within cyaA at 448.001 kb on - strand, within cyaA at 448.148 kb on + strand, within cyaA at 448.166 kb on - strand, within cyaA at 448.182 kb on - strand, within cyaA at 448.254 kb on + strand, within cyaA at 448.254 kb on + strand, within cyaA at 448.262 kb on - strand, within cyaA at 448.269 kb on + strand, within cyaA at 448.343 kb on - strand, within cyaA at 448.477 kb on - strand, within cyaA at 448.711 kb on + strand, within cyaA at 448.811 kb on + strand, within cyaA at 448.819 kb on - strand, within cyaA at 448.924 kb on - strand, within cyaA at 448.924 kb on - strand, within cyaA at 448.928 kb on + strand, within cyaA at 448.935 kb on + strand, within cyaA at 449.013 kb on + strand, within cyaA at 449.063 kb on - strand, within cyaA at 449.111 kb on - strand, within cyaA at 449.111 kb on - strand, within cyaA at 449.111 kb on - strand, within cyaA at 449.275 kb on - strand, within cyaA at 449.579 kb on + strand, within cyaA at 449.602 kb on + strand, within cyaA at 449.853 kb on + strand at 449.931 kb on + strand at 449.931 kb on + strand at 449.931 kb on + strand at 449.931 kb on + strand at 450.004 kb on - strand at 450.131 kb on - strand, within Shewana3_0388 at 450.142 kb on + strand, within Shewana3_0388 at 450.568 kb on + strand, within Shewana3_0389 at 450.568 kb on + strand, within Shewana3_0389 at 450.576 kb on - strand, within Shewana3_0389 at 450.589 kb on + strand, within Shewana3_0389 at 450.658 kb on + strand, within Shewana3_0389 at 450.746 kb on + strand at 450.755 kb on - strand at 450.793 kb on + strand at 450.796 kb on + strand at 450.806 kb on + strand at 450.823 kb on - strand at 450.839 kb on + strand at 450.899 kb on + strand, within Shewana3_0390 at 450.940 kb on - strand, within Shewana3_0390 at 450.999 kb on - strand, within Shewana3_0390 at 451.036 kb on + strand, within Shewana3_0390
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source D,L-Lactate 10 mM remove 447,707 - -0.3 447,738 - -0.3 447,852 - -0.4 447,941 - cyaA Shewana3_0387 0.13 -1.4 447,977 - cyaA Shewana3_0387 0.14 -0.8 447,986 + cyaA Shewana3_0387 0.14 -0.7 447,994 - cyaA Shewana3_0387 0.15 -1.3 447,994 - cyaA Shewana3_0387 0.15 -0.8 448,001 - cyaA Shewana3_0387 0.15 -0.5 448,148 + cyaA Shewana3_0387 0.21 -0.7 448,166 - cyaA Shewana3_0387 0.22 -1.2 448,182 - cyaA Shewana3_0387 0.23 -1.1 448,254 + cyaA Shewana3_0387 0.26 +0.2 448,254 + cyaA Shewana3_0387 0.26 +0.7 448,262 - cyaA Shewana3_0387 0.26 -0.5 448,269 + cyaA Shewana3_0387 0.26 -0.7 448,343 - cyaA Shewana3_0387 0.29 -0.0 448,477 - cyaA Shewana3_0387 0.35 -1.7 448,711 + cyaA Shewana3_0387 0.44 -0.4 448,811 + cyaA Shewana3_0387 0.49 -2.8 448,819 - cyaA Shewana3_0387 0.49 -0.5 448,924 - cyaA Shewana3_0387 0.53 -1.5 448,924 - cyaA Shewana3_0387 0.53 +0.2 448,928 + cyaA Shewana3_0387 0.53 -1.1 448,935 + cyaA Shewana3_0387 0.54 -1.2 449,013 + cyaA Shewana3_0387 0.57 -0.7 449,063 - cyaA Shewana3_0387 0.59 -0.7 449,111 - cyaA Shewana3_0387 0.61 -0.3 449,111 - cyaA Shewana3_0387 0.61 -0.9 449,111 - cyaA Shewana3_0387 0.61 -0.5 449,275 - cyaA Shewana3_0387 0.68 -1.6 449,579 + cyaA Shewana3_0387 0.80 -1.2 449,602 + cyaA Shewana3_0387 0.81 -0.8 449,853 + -0.8 449,931 + -0.7 449,931 + -0.2 449,931 + -1.3 449,931 + -0.8 450,004 - -1.5 450,131 - Shewana3_0388 0.12 -1.7 450,142 + Shewana3_0388 0.15 -2.5 450,568 + Shewana3_0389 0.41 +0.1 450,568 + Shewana3_0389 0.41 +0.2 450,576 - Shewana3_0389 0.45 -2.8 450,589 + Shewana3_0389 0.52 +0.1 450,658 + Shewana3_0389 0.87 -1.4 450,746 + -5.8 450,755 - -4.5 450,793 + -4.0 450,796 + -1.6 450,806 + -4.0 450,823 - -5.2 450,839 + -4.1 450,899 + Shewana3_0390 0.14 -4.3 450,940 - Shewana3_0390 0.17 -3.3 450,999 - Shewana3_0390 0.22 +1.7 451,036 + Shewana3_0390 0.25 -4.8
Or see this region's nucleotide sequence