Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS37380 and RR42_RS37385 are separated by 20 nucleotides RR42_RS37385 and RR42_RS37390 overlap by 1 nucleotides RR42_RS37390 and RR42_RS37395 are separated by 13 nucleotides
RR42_RS37380: RR42_RS37380 - 2-hydroxyacid dehydrogenase, at 3,864,413 to 3,865,348
_RS37380
RR42_RS37385: RR42_RS37385 - membrane protein, at 3,865,369 to 3,866,742
_RS37385
RR42_RS37390: RR42_RS37390 - XRE family transcriptional regulator, at 3,866,742 to 3,867,440
_RS37390
RR42_RS37395: RR42_RS37395 - glucokinase, at 3,867,454 to 3,868,491
_RS37395
Position (kb)
3865
3866
3867 Strain fitness (log2 ratio)
-2
-1
0
1 at 3864.480 kb on - strand at 3864.480 kb on - strand at 3864.480 kb on - strand at 3864.641 kb on + strand, within RR42_RS37380 at 3864.953 kb on + strand, within RR42_RS37380 at 3864.960 kb on - strand, within RR42_RS37380 at 3864.998 kb on + strand, within RR42_RS37380 at 3864.998 kb on + strand, within RR42_RS37380 at 3864.999 kb on - strand, within RR42_RS37380 at 3864.999 kb on - strand, within RR42_RS37380 at 3864.999 kb on - strand, within RR42_RS37380 at 3865.176 kb on + strand, within RR42_RS37380 at 3865.176 kb on + strand, within RR42_RS37380 at 3865.177 kb on - strand, within RR42_RS37380 at 3865.177 kb on - strand, within RR42_RS37380 at 3865.177 kb on - strand, within RR42_RS37380 at 3865.178 kb on + strand, within RR42_RS37380 at 3865.512 kb on - strand, within RR42_RS37385 at 3865.512 kb on - strand, within RR42_RS37385 at 3865.606 kb on + strand, within RR42_RS37385 at 3865.606 kb on + strand, within RR42_RS37385 at 3865.667 kb on + strand, within RR42_RS37385 at 3865.667 kb on + strand, within RR42_RS37385 at 3865.667 kb on + strand, within RR42_RS37385 at 3865.667 kb on + strand, within RR42_RS37385 at 3865.668 kb on - strand, within RR42_RS37385 at 3865.668 kb on - strand, within RR42_RS37385 at 3865.668 kb on - strand, within RR42_RS37385 at 3865.965 kb on + strand, within RR42_RS37385 at 3866.363 kb on + strand, within RR42_RS37385 at 3866.363 kb on + strand, within RR42_RS37385 at 3866.363 kb on + strand, within RR42_RS37385 at 3866.363 kb on + strand, within RR42_RS37385 at 3866.364 kb on - strand, within RR42_RS37385 at 3866.364 kb on - strand, within RR42_RS37385 at 3866.364 kb on - strand, within RR42_RS37385 at 3866.450 kb on + strand, within RR42_RS37385 at 3866.451 kb on - strand, within RR42_RS37385 at 3866.479 kb on - strand, within RR42_RS37385 at 3866.479 kb on - strand, within RR42_RS37385 at 3866.481 kb on - strand, within RR42_RS37385 at 3866.622 kb on - strand at 3866.661 kb on - strand at 3866.661 kb on - strand at 3866.779 kb on + strand at 3866.780 kb on - strand at 3866.780 kb on - strand at 3866.795 kb on + strand at 3866.842 kb on + strand, within RR42_RS37390 at 3866.843 kb on - strand, within RR42_RS37390 at 3866.884 kb on + strand, within RR42_RS37390 at 3867.127 kb on + strand, within RR42_RS37390 at 3867.286 kb on + strand, within RR42_RS37390 at 3867.286 kb on + strand, within RR42_RS37390 at 3867.286 kb on + strand, within RR42_RS37390 at 3867.287 kb on - strand, within RR42_RS37390 at 3867.287 kb on - strand, within RR42_RS37390 at 3867.393 kb on + strand at 3867.394 kb on - strand at 3867.465 kb on + strand at 3867.465 kb on + strand at 3867.465 kb on + strand at 3867.466 kb on - strand at 3867.466 kb on - strand at 3867.466 kb on - strand at 3867.588 kb on - strand, within RR42_RS37395 at 3867.588 kb on - strand, within RR42_RS37395 at 3867.609 kb on - strand, within RR42_RS37395 at 3867.609 kb on - strand, within RR42_RS37395 at 3867.609 kb on - strand, within RR42_RS37395 at 3867.701 kb on + strand, within RR42_RS37395
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 3,864,480 - +0.2 3,864,480 - -0.2 3,864,480 - +0.0 3,864,641 + RR42_RS37380 0.24 +0.5 3,864,953 + RR42_RS37380 0.58 +0.2 3,864,960 - RR42_RS37380 0.58 -0.2 3,864,998 + RR42_RS37380 0.62 +0.2 3,864,998 + RR42_RS37380 0.62 +0.8 3,864,999 - RR42_RS37380 0.63 -1.1 3,864,999 - RR42_RS37380 0.63 -0.0 3,864,999 - RR42_RS37380 0.63 -0.3 3,865,176 + RR42_RS37380 0.82 -0.3 3,865,176 + RR42_RS37380 0.82 -0.9 3,865,177 - RR42_RS37380 0.82 -0.7 3,865,177 - RR42_RS37380 0.82 +0.6 3,865,177 - RR42_RS37380 0.82 -0.3 3,865,178 + RR42_RS37380 0.82 -0.9 3,865,512 - RR42_RS37385 0.10 -0.1 3,865,512 - RR42_RS37385 0.10 -1.2 3,865,606 + RR42_RS37385 0.17 +0.3 3,865,606 + RR42_RS37385 0.17 -0.6 3,865,667 + RR42_RS37385 0.22 -0.9 3,865,667 + RR42_RS37385 0.22 -1.5 3,865,667 + RR42_RS37385 0.22 -0.4 3,865,667 + RR42_RS37385 0.22 -1.6 3,865,668 - RR42_RS37385 0.22 -0.4 3,865,668 - RR42_RS37385 0.22 -0.5 3,865,668 - RR42_RS37385 0.22 +0.7 3,865,965 + RR42_RS37385 0.43 +0.6 3,866,363 + RR42_RS37385 0.72 +0.2 3,866,363 + RR42_RS37385 0.72 -1.0 3,866,363 + RR42_RS37385 0.72 -1.1 3,866,363 + RR42_RS37385 0.72 -0.8 3,866,364 - RR42_RS37385 0.72 -0.8 3,866,364 - RR42_RS37385 0.72 +0.1 3,866,364 - RR42_RS37385 0.72 -0.9 3,866,450 + RR42_RS37385 0.79 +0.3 3,866,451 - RR42_RS37385 0.79 -0.1 3,866,479 - RR42_RS37385 0.81 -0.1 3,866,479 - RR42_RS37385 0.81 -0.6 3,866,481 - RR42_RS37385 0.81 -0.9 3,866,622 - +0.1 3,866,661 - -1.2 3,866,661 - -0.5 3,866,779 + -0.5 3,866,780 - +0.1 3,866,780 - -0.4 3,866,795 + -0.2 3,866,842 + RR42_RS37390 0.14 +0.4 3,866,843 - RR42_RS37390 0.14 +0.2 3,866,884 + RR42_RS37390 0.20 -0.7 3,867,127 + RR42_RS37390 0.55 -0.2 3,867,286 + RR42_RS37390 0.78 -0.2 3,867,286 + RR42_RS37390 0.78 +0.3 3,867,286 + RR42_RS37390 0.78 -1.8 3,867,287 - RR42_RS37390 0.78 -0.8 3,867,287 - RR42_RS37390 0.78 +1.2 3,867,393 + -0.3 3,867,394 - -0.6 3,867,465 + +1.1 3,867,465 + -0.8 3,867,465 + +1.5 3,867,466 - +0.0 3,867,466 - -0.2 3,867,466 - -0.2 3,867,588 - RR42_RS37395 0.13 -0.4 3,867,588 - RR42_RS37395 0.13 +0.3 3,867,609 - RR42_RS37395 0.15 -1.2 3,867,609 - RR42_RS37395 0.15 +0.9 3,867,609 - RR42_RS37395 0.15 +0.5 3,867,701 + RR42_RS37395 0.24 +0.1
Or see this region's nucleotide sequence