Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35745 and RR42_RS35750 overlap by 4 nucleotides RR42_RS35750 and RR42_RS35755 are separated by 40 nucleotides RR42_RS35755 and RR42_RS35760 are separated by 171 nucleotides
RR42_RS35745: RR42_RS35745 - flagellar biosynthesis protein FlhE, at 3,467,211 to 3,467,642
_RS35745
RR42_RS35750: RR42_RS35750 - DNA-directed RNA polymerase sigma-70 factor, at 3,467,639 to 3,468,355
_RS35750
RR42_RS35755: RR42_RS35755 - flagellar biosynthesis protein FlhG, at 3,468,396 to 3,469,172
_RS35755
RR42_RS35760: RR42_RS35760 - flagellar biosynthesis regulator FlhF, at 3,469,344 to 3,471,659
_RS35760
Position (kb)
3468
3469
3470 Strain fitness (log2 ratio)
-1
0
1 at 3467.470 kb on + strand, within RR42_RS35745 at 3467.552 kb on + strand, within RR42_RS35745 at 3467.552 kb on + strand, within RR42_RS35745 at 3467.552 kb on + strand, within RR42_RS35745 at 3467.552 kb on + strand, within RR42_RS35745 at 3467.552 kb on + strand, within RR42_RS35745 at 3467.553 kb on - strand, within RR42_RS35745 at 3467.761 kb on - strand, within RR42_RS35750 at 3467.774 kb on + strand, within RR42_RS35750 at 3467.928 kb on + strand, within RR42_RS35750 at 3467.928 kb on + strand, within RR42_RS35750 at 3467.928 kb on + strand, within RR42_RS35750 at 3467.928 kb on + strand, within RR42_RS35750 at 3467.929 kb on - strand, within RR42_RS35750 at 3467.929 kb on - strand, within RR42_RS35750 at 3467.929 kb on - strand, within RR42_RS35750 at 3467.950 kb on - strand, within RR42_RS35750 at 3467.950 kb on - strand, within RR42_RS35750 at 3467.950 kb on - strand at 3468.156 kb on + strand, within RR42_RS35750 at 3468.157 kb on - strand, within RR42_RS35750 at 3468.157 kb on - strand, within RR42_RS35750 at 3468.349 kb on + strand at 3468.350 kb on - strand at 3468.350 kb on - strand at 3468.351 kb on + strand at 3468.351 kb on + strand at 3468.351 kb on + strand at 3468.351 kb on + strand at 3468.351 kb on + strand at 3468.352 kb on - strand at 3468.376 kb on + strand at 3468.376 kb on + strand at 3468.377 kb on - strand at 3468.616 kb on + strand, within RR42_RS35755 at 3468.616 kb on + strand, within RR42_RS35755 at 3468.616 kb on + strand, within RR42_RS35755 at 3468.713 kb on - strand, within RR42_RS35755 at 3468.742 kb on + strand, within RR42_RS35755 at 3468.742 kb on + strand, within RR42_RS35755 at 3468.743 kb on - strand, within RR42_RS35755 at 3468.743 kb on - strand, within RR42_RS35755 at 3468.849 kb on - strand, within RR42_RS35755 at 3469.220 kb on - strand at 3469.220 kb on - strand at 3469.220 kb on - strand at 3469.220 kb on - strand at 3469.220 kb on - strand at 3469.712 kb on - strand, within RR42_RS35760 at 3469.873 kb on + strand, within RR42_RS35760 at 3470.040 kb on + strand, within RR42_RS35760 at 3470.040 kb on + strand, within RR42_RS35760 at 3470.041 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 3,467,470 + RR42_RS35745 0.60 +0.1 3,467,552 + RR42_RS35745 0.79 -0.8 3,467,552 + RR42_RS35745 0.79 -0.5 3,467,552 + RR42_RS35745 0.79 -0.0 3,467,552 + RR42_RS35745 0.79 -0.6 3,467,552 + RR42_RS35745 0.79 +0.8 3,467,553 - RR42_RS35745 0.79 -0.7 3,467,761 - RR42_RS35750 0.17 +0.5 3,467,774 + RR42_RS35750 0.19 +1.1 3,467,928 + RR42_RS35750 0.40 -0.2 3,467,928 + RR42_RS35750 0.40 -0.9 3,467,928 + RR42_RS35750 0.40 -0.7 3,467,928 + RR42_RS35750 0.40 -0.8 3,467,929 - RR42_RS35750 0.40 +0.1 3,467,929 - RR42_RS35750 0.40 -0.4 3,467,929 - RR42_RS35750 0.40 -1.4 3,467,950 - RR42_RS35750 0.43 -0.9 3,467,950 - RR42_RS35750 0.43 -1.1 3,467,950 - +0.1 3,468,156 + RR42_RS35750 0.72 -1.0 3,468,157 - RR42_RS35750 0.72 +0.5 3,468,157 - RR42_RS35750 0.72 +0.2 3,468,349 + +1.7 3,468,350 - -0.4 3,468,350 - +0.5 3,468,351 + -0.8 3,468,351 + -0.3 3,468,351 + -0.6 3,468,351 + +0.4 3,468,351 + -0.9 3,468,352 - -1.1 3,468,376 + +0.2 3,468,376 + -1.5 3,468,377 - -0.7 3,468,616 + RR42_RS35755 0.28 -0.5 3,468,616 + RR42_RS35755 0.28 -0.2 3,468,616 + RR42_RS35755 0.28 -0.2 3,468,713 - RR42_RS35755 0.41 -0.9 3,468,742 + RR42_RS35755 0.45 -0.2 3,468,742 + RR42_RS35755 0.45 -0.8 3,468,743 - RR42_RS35755 0.45 -1.3 3,468,743 - RR42_RS35755 0.45 -1.3 3,468,849 - RR42_RS35755 0.58 +0.4 3,469,220 - +0.8 3,469,220 - +0.3 3,469,220 - +0.9 3,469,220 - -0.7 3,469,220 - -0.5 3,469,712 - RR42_RS35760 0.16 +0.0 3,469,873 + RR42_RS35760 0.23 -0.9 3,470,040 + RR42_RS35760 0.30 -0.6 3,470,040 + RR42_RS35760 0.30 -1.1 3,470,041 - +0.6
Or see this region's nucleotide sequence