Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35235 and RR42_RS35240 are separated by 445 nucleotides RR42_RS35240 and RR42_RS35245 are separated by 38 nucleotides
RR42_RS35235: RR42_RS35235 - glutathione ABC transporter permease, at 3,357,913 to 3,358,869
_RS35235
RR42_RS35240: RR42_RS35240 - acyl-CoA dehydrogenase, at 3,359,315 to 3,360,454
_RS35240
RR42_RS35245: RR42_RS35245 - acyl-CoA dehydrogenase, at 3,360,493 to 3,361,677
_RS35245
Position (kb)
3359
3360
3361 Strain fitness (log2 ratio)
-2
-1
0
1 at 3358.358 kb on + strand, within RR42_RS35235 at 3358.469 kb on + strand, within RR42_RS35235 at 3358.859 kb on + strand at 3358.859 kb on + strand at 3358.859 kb on + strand at 3358.860 kb on - strand at 3358.860 kb on - strand at 3358.860 kb on - strand at 3359.160 kb on - strand at 3359.160 kb on - strand at 3359.160 kb on - strand at 3359.160 kb on - strand at 3359.160 kb on - strand at 3359.173 kb on - strand at 3359.173 kb on - strand at 3359.173 kb on - strand at 3359.400 kb on + strand at 3359.400 kb on + strand at 3359.401 kb on - strand at 3359.668 kb on - strand, within RR42_RS35240 at 3359.869 kb on + strand, within RR42_RS35240 at 3359.968 kb on + strand, within RR42_RS35240 at 3360.268 kb on - strand, within RR42_RS35240 at 3360.699 kb on - strand, within RR42_RS35245 at 3360.701 kb on + strand, within RR42_RS35245 at 3360.702 kb on - strand, within RR42_RS35245 at 3360.755 kb on - strand, within RR42_RS35245 at 3360.948 kb on - strand, within RR42_RS35245 at 3361.179 kb on - strand, within RR42_RS35245 at 3361.179 kb on - strand, within RR42_RS35245 at 3361.190 kb on - strand, within RR42_RS35245
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 3,358,358 + RR42_RS35235 0.46 +0.4 3,358,469 + RR42_RS35235 0.58 -0.2 3,358,859 + -0.9 3,358,859 + +0.1 3,358,859 + +0.2 3,358,860 - -1.3 3,358,860 - +0.6 3,358,860 - -0.5 3,359,160 - +0.5 3,359,160 - -1.2 3,359,160 - +1.1 3,359,160 - -1.6 3,359,160 - +0.3 3,359,173 - +1.4 3,359,173 - -0.1 3,359,173 - +1.5 3,359,400 + +0.0 3,359,400 + +0.1 3,359,401 - -0.1 3,359,668 - RR42_RS35240 0.31 -0.3 3,359,869 + RR42_RS35240 0.49 -1.2 3,359,968 + RR42_RS35240 0.57 -0.6 3,360,268 - RR42_RS35240 0.84 -0.5 3,360,699 - RR42_RS35245 0.17 +0.4 3,360,701 + RR42_RS35245 0.18 +1.0 3,360,702 - RR42_RS35245 0.18 +0.2 3,360,755 - RR42_RS35245 0.22 +0.1 3,360,948 - RR42_RS35245 0.38 -0.2 3,361,179 - RR42_RS35245 0.58 +0.3 3,361,179 - RR42_RS35245 0.58 -2.2 3,361,190 - RR42_RS35245 0.59 -0.1
Or see this region's nucleotide sequence