Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS34960 and RR42_RS34965 are separated by 258 nucleotides RR42_RS34965 and RR42_RS34970 are separated by 61 nucleotides
RR42_RS34960: RR42_RS34960 - membrane protein, at 3,294,432 to 3,294,935
_RS34960
RR42_RS34965: RR42_RS34965 - dioxygenase, at 3,295,194 to 3,296,729
_RS34965
RR42_RS34970: RR42_RS34970 - bifunctional D-altronate/D-mannonate dehydratase, at 3,296,791 to 3,298,002
_RS34970
Position (kb)
3295
3296
3297 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3294.307 kb on - strand at 3294.307 kb on - strand at 3294.441 kb on + strand at 3294.441 kb on + strand at 3294.441 kb on + strand at 3294.441 kb on + strand at 3294.442 kb on - strand at 3294.442 kb on - strand at 3294.512 kb on + strand, within RR42_RS34960 at 3294.513 kb on - strand, within RR42_RS34960 at 3294.513 kb on - strand, within RR42_RS34960 at 3294.563 kb on - strand, within RR42_RS34960 at 3294.563 kb on - strand, within RR42_RS34960 at 3294.907 kb on + strand at 3294.936 kb on - strand at 3294.950 kb on - strand at 3295.014 kb on + strand at 3295.015 kb on - strand at 3295.015 kb on - strand at 3295.052 kb on - strand at 3295.064 kb on + strand at 3295.064 kb on + strand at 3295.064 kb on + strand at 3295.065 kb on - strand at 3295.065 kb on - strand at 3295.065 kb on - strand at 3295.065 kb on - strand at 3295.105 kb on + strand at 3295.105 kb on + strand at 3295.172 kb on + strand at 3295.172 kb on + strand at 3295.427 kb on + strand, within RR42_RS34965 at 3295.595 kb on + strand, within RR42_RS34965 at 3295.596 kb on - strand, within RR42_RS34965 at 3295.596 kb on - strand, within RR42_RS34965 at 3296.468 kb on - strand, within RR42_RS34965 at 3296.676 kb on + strand at 3296.676 kb on + strand at 3296.676 kb on + strand at 3296.676 kb on + strand at 3296.677 kb on - strand at 3296.677 kb on - strand at 3296.792 kb on + strand at 3296.793 kb on - strand at 3296.840 kb on + strand at 3296.915 kb on + strand, within RR42_RS34970 at 3296.984 kb on + strand, within RR42_RS34970 at 3296.985 kb on - strand, within RR42_RS34970 at 3297.162 kb on - strand, within RR42_RS34970 at 3297.412 kb on + strand, within RR42_RS34970 at 3297.413 kb on - strand, within RR42_RS34970 at 3297.413 kb on - strand, within RR42_RS34970 at 3297.528 kb on - strand, within RR42_RS34970 at 3297.528 kb on - strand, within RR42_RS34970 at 3297.528 kb on - strand, within RR42_RS34970 at 3297.537 kb on + strand, within RR42_RS34970
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 3,294,307 - -1.3 3,294,307 - +1.8 3,294,441 + -0.3 3,294,441 + +0.0 3,294,441 + +0.3 3,294,441 + +0.7 3,294,442 - -2.4 3,294,442 - +0.3 3,294,512 + RR42_RS34960 0.16 -0.4 3,294,513 - RR42_RS34960 0.16 +0.1 3,294,513 - RR42_RS34960 0.16 +0.0 3,294,563 - RR42_RS34960 0.26 -0.9 3,294,563 - RR42_RS34960 0.26 -0.0 3,294,907 + +0.5 3,294,936 - +0.4 3,294,950 - -0.2 3,295,014 + -0.0 3,295,015 - +0.1 3,295,015 - +0.3 3,295,052 - -0.1 3,295,064 + +0.4 3,295,064 + -0.5 3,295,064 + +2.5 3,295,065 - +1.2 3,295,065 - +0.4 3,295,065 - -0.4 3,295,065 - -0.3 3,295,105 + +1.0 3,295,105 + -0.4 3,295,172 + -0.4 3,295,172 + +1.3 3,295,427 + RR42_RS34965 0.15 -1.0 3,295,595 + RR42_RS34965 0.26 -1.2 3,295,596 - RR42_RS34965 0.26 -0.6 3,295,596 - RR42_RS34965 0.26 -0.0 3,296,468 - RR42_RS34965 0.83 -0.8 3,296,676 + -0.3 3,296,676 + +0.1 3,296,676 + +0.4 3,296,676 + -0.1 3,296,677 - -0.9 3,296,677 - -0.5 3,296,792 + +0.5 3,296,793 - +2.4 3,296,840 + -1.3 3,296,915 + RR42_RS34970 0.10 -0.9 3,296,984 + RR42_RS34970 0.16 +0.2 3,296,985 - RR42_RS34970 0.16 -0.3 3,297,162 - RR42_RS34970 0.31 +0.3 3,297,412 + RR42_RS34970 0.51 +0.0 3,297,413 - RR42_RS34970 0.51 +0.1 3,297,413 - RR42_RS34970 0.51 -0.4 3,297,528 - RR42_RS34970 0.61 +0.2 3,297,528 - RR42_RS34970 0.61 +0.1 3,297,528 - RR42_RS34970 0.61 -0.3 3,297,537 + RR42_RS34970 0.62 -0.3
Or see this region's nucleotide sequence