Experiment: R2A with Cephalothin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS34585 and RR42_RS34590 are separated by 242 nucleotides RR42_RS34590 and RR42_RS34595 are separated by 58 nucleotides
RR42_RS34585: RR42_RS34585 - LysR family transcriptional regulator, at 3,200,203 to 3,201,114
_RS34585
RR42_RS34590: RR42_RS34590 - glutathione S-transferase, at 3,201,357 to 3,201,971
_RS34590
RR42_RS34595: RR42_RS34595 - FAD-binding oxidoreductase, at 3,202,030 to 3,204,144
_RS34595
Position (kb)
3201
3202 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3200.395 kb on + strand, within RR42_RS34585 at 3200.396 kb on - strand, within RR42_RS34585 at 3201.155 kb on + strand at 3201.200 kb on + strand at 3201.200 kb on + strand at 3201.200 kb on + strand at 3201.200 kb on + strand at 3201.201 kb on - strand at 3201.201 kb on - strand at 3201.366 kb on + strand at 3201.366 kb on + strand at 3201.567 kb on + strand, within RR42_RS34590 at 3201.567 kb on + strand, within RR42_RS34590 at 3201.567 kb on + strand, within RR42_RS34590 at 3201.568 kb on - strand, within RR42_RS34590 at 3201.568 kb on - strand, within RR42_RS34590 at 3201.714 kb on + strand, within RR42_RS34590 at 3201.714 kb on + strand, within RR42_RS34590 at 3201.715 kb on - strand, within RR42_RS34590 at 3201.831 kb on + strand, within RR42_RS34590 at 3201.832 kb on - strand, within RR42_RS34590 at 3201.832 kb on - strand, within RR42_RS34590 at 3201.832 kb on - strand, within RR42_RS34590 at 3201.832 kb on - strand, within RR42_RS34590 at 3201.837 kb on + strand, within RR42_RS34590 at 3201.837 kb on + strand, within RR42_RS34590 at 3201.837 kb on + strand, within RR42_RS34590 at 3201.838 kb on - strand, within RR42_RS34590 at 3201.838 kb on - strand, within RR42_RS34590 at 3202.168 kb on + strand at 3202.168 kb on + strand at 3202.169 kb on - strand at 3202.499 kb on - strand, within RR42_RS34595 at 3202.639 kb on + strand, within RR42_RS34595 at 3202.640 kb on - strand, within RR42_RS34595
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cephalothin 0.25 mg/ml remove 3,200,395 + RR42_RS34585 0.21 +0.7 3,200,396 - RR42_RS34585 0.21 +0.7 3,201,155 + -0.4 3,201,200 + -1.0 3,201,200 + +0.0 3,201,200 + -2.3 3,201,200 + +0.3 3,201,201 - +0.7 3,201,201 - +2.6 3,201,366 + +0.2 3,201,366 + +0.8 3,201,567 + RR42_RS34590 0.34 -0.5 3,201,567 + RR42_RS34590 0.34 +0.3 3,201,567 + RR42_RS34590 0.34 +0.0 3,201,568 - RR42_RS34590 0.34 +0.3 3,201,568 - RR42_RS34590 0.34 +0.3 3,201,714 + RR42_RS34590 0.58 -0.4 3,201,714 + RR42_RS34590 0.58 -0.8 3,201,715 - RR42_RS34590 0.58 +0.3 3,201,831 + RR42_RS34590 0.77 +1.5 3,201,832 - RR42_RS34590 0.77 +1.8 3,201,832 - RR42_RS34590 0.77 -0.1 3,201,832 - RR42_RS34590 0.77 -0.3 3,201,832 - RR42_RS34590 0.77 +0.4 3,201,837 + RR42_RS34590 0.78 +0.4 3,201,837 + RR42_RS34590 0.78 +0.8 3,201,837 + RR42_RS34590 0.78 +0.2 3,201,838 - RR42_RS34590 0.78 +0.4 3,201,838 - RR42_RS34590 0.78 +0.1 3,202,168 + -0.7 3,202,168 + +0.8 3,202,169 - -1.3 3,202,499 - RR42_RS34595 0.22 +0.8 3,202,639 + RR42_RS34595 0.29 -0.2 3,202,640 - RR42_RS34595 0.29 +0.7
Or see this region's nucleotide sequence